A kind of medicinal bletilla striata tissue culture seedling propagation method
A tissue culture and tissue culture seedling technology, applied in the field of plant reproduction, can solve the problems of inability to meet the needs of large-scale planting, high variation ratio, weak seedlings, etc., and achieve the effect of satisfying large-scale planting and efficient seedling breeding.
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Embodiment 1
[0046] Embodiment 1: A method for breeding medicinal Bletilla striata tissue culture seedlings, comprising the following cultivation steps: S1: Explant selection, selecting the tissue culture seedlings that germinate and take root from sterile naked embryos as explants, and keeping the explants in the Sterile state; S2: Proliferation culture, part or all of the leaves and roots of the explants in the sterile state were excised, inoculated in solid proliferation medium, entered the dark culture stage for 2 days, and then entered the light culture stage for 8 weeks of proliferation , the proliferation rate is controlled at 3 times, and the multiplication obtains the buds;
[0047] S3: Strong seedling cultivation, every 12 weeks, divide the buds cultivated in the multiplication stage into single buds, and cut off part or all of the leaves, transfer them to the pre-made strong seedling medium, culture in dark for 2 days, and then light Cultivate for 2 weeks; S4: rooting induction,...
Embodiment 2
[0070] Embodiment 2: A method for breeding medicinal Bletilla striata tissue culture seedlings, the difference from Example 1 is that S2: Proliferation culture, part or all of the leaves and roots of the explants in the sterile state are excised, and inoculated on solid In the proliferation medium, keep the temperature at 25°C, enter the dark culture stage for 2 days, and then enter the light culture stage for 8 weeks. The light intensity is controlled at 1500Lux, the photoperiod is 12 / 8h, and the proliferation rate is controlled at 3 S3: Cultivation of strong seedlings. Every 12 weeks, divide the buds cultivated in the multiplication stage into single buds, cut off part or all of the leaves, and transfer the buds to the medium for strong seedlings. Maintain the temperature at 28°C, culture in the dark for 2 days, and cultivate in the light for 8 weeks. The light intensity is controlled at 2000 Lux, and the photoperiod is 12 / 8h. Take the healthy single buds as the tissue cultur...
Embodiment 3
[0071] Embodiment 3: A kind of medicinal Bletilla striata tissue culture seedling breeding method, the difference with embodiment 1 or 2 is that, S2: Proliferation culture, part or all of the leaves and roots of the explants in the aseptic state are excised, and inoculated In the solid proliferation medium, keep the temperature at 25°C, enter the dark culture stage for 7 days, and then enter the light culture stage for 16 weeks of proliferation and culture. The light intensity is controlled at 3000 Lux, the photoperiod is 20 / 8h, and the proliferation rate is controlled. At 3 times, proliferate to obtain cluster buds; S4: cultivate strong seedlings, repeat S3, remove part or all leaves of the cut cluster buds, and transfer the cluster buds to strong seedling medium, maintain the temperature at 28 °C, and keep the temperature in the dark Cultivate for 7 days, light culture for 16 weeks, light intensity is controlled at 3000Lux, photoperiod is 20 / 8h, take the single bud that grows...
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