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A method for directly regenerating plants from Chinese toon leaves

A technology for regenerating plants and leaves, applied in the biological field, can solve the problems of low reproduction coefficient, browning, cumbersome regeneration process, etc., and achieve the effects of high reproduction coefficient, high regeneration efficiency and fast regeneration speed

Active Publication Date: 2018-06-19
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The former has problems such as pollution, serious browning, and low reproduction coefficient during the breeding process; the latter has problems such as cumbersome regeneration process, low callus differentiation efficiency, and difficulty in maintaining excellent agronomic traits of the female parent.

Method used

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  • A method for directly regenerating plants from Chinese toon leaves
  • A method for directly regenerating plants from Chinese toon leaves
  • A method for directly regenerating plants from Chinese toon leaves

Examples

Experimental program
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Effect test

Embodiment 1

[0040] A method for directly regenerating plants from Chinese toon leaves, such as figure 1 shown, including the following steps:

[0041] (1) Take materials

[0042] Select the young leaves of current-year shoots of the perennial Toona sinensis excellent strain with high-yield characteristics in the field as explants;

[0043] (2) Pretreatment of explants

[0044] Soak the Toona sinensis leaf in the step (1) in 0.4% potassium permanganate solution for 2 minutes, rinse with running water for 10 minutes, and rinse 2 times with sterile water in an aseptic operating table;

[0045] (3) Surface disinfection of explants

[0046] Surface disinfection of the pretreated Chinese toon leaves: first sterilize the pretreated Chinese toon leaves with 70% absolute ethanol for 15 seconds, then rinse with sterile water for 3 times; repeat once; finally, use 8% peroxide Disinfect with hydrogen solution for 3 minutes, rinse with sterile water 5 times;

[0047] (4) Induction of adventitious...

Embodiment 2

[0056] A method for directly regenerating plants from Chinese toon leaves, such as figure 1 shown, including the following steps:

[0057] (1) Take materials

[0058] Select the young leaves of the current year's branches of the excellent perennial Toona sinensis strain in the field as explants;

[0059] (2) Pretreatment of explants

[0060] Soak the Toona sinensis leaf in the step (1) in 0.5% potassium permanganate solution for 4 minutes, rinse with running water for 15 minutes, and rinse 2 times with sterile water in an aseptic operating table;

[0061] (3) Surface disinfection of explants

[0062] Surface disinfection of the pretreated Chinese toon leaves: first sterilize the pretreated Chinese toon leaves with 73% absolute ethanol for 20 seconds, then rinse with sterile water 4 times; repeat 2 times; finally, use 10% peroxide Disinfect with hydrogen solution for 4 minutes, rinse with sterile water 6 times;

[0063] (4) Induction of adventitious buds

[0064] The Toon...

Embodiment 3

[0072] A method for directly regenerating plants from Chinese toon leaves, such as figure 1 shown, including the following steps:

[0073] (1) Take materials

[0074] Select the young leaves of current-year shoots of the perennial Toona sinensis excellent strains with high resistance characteristics in the field as explants;

[0075] (2) Pretreatment of explants

[0076] Soak the Toona sinensis leaf in the step (1) in 0.6% potassium permanganate solution for 5 minutes, rinse with running water for 20 minutes, and rinse 3 times with sterile water in an aseptic operating table;

[0077] (3) Surface disinfection of explants

[0078] Surface disinfection of the pretreated Chinese toon leaves: first sterilize the pretreated Chinese toon leaves with 75% absolute ethanol for 25 seconds, then rinse 5 times with sterile water; repeat 3 times; finally, use 12% peroxide Disinfect with hydrogen solution for 5 minutes, rinse with sterile water 6 times;

[0079] (4) Induction of advent...

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Abstract

The invention discloses a method for direct regenerating plant of cedrela sinensis leaf. The method includes steps of taking materials, pretreating an explant, disinfecting the surface of the explant, inducing an adventitious bud, multiplying the adventitious bud, elongating the adventitious bud and rooting the adventitious bud. The method has the advantages that (1), the material is convenient to take and rich in source; (2) the regeneration efficiency is high, and the inducing rate of the adventitious bud is up to 88.6% and the rooting rate is up to 94.7%; (3) the regeneration speed is fast and the propagation coefficient is high; moreover, the further enlarged propagation of the aseptic seedling leaf is realized, and important technical support for the rapid propagation and variety improvement of an excellent cedrela sinensis plant is provided; therefore, the method for direct regenerating plant of cedrela sinensis leaf not only provides the technical support for the rapid propagation and scaled production of excellent cedrela sinensis system but also lays a foundation for the variety improvement of cedrela sinensis in the later period.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for directly regenerating plants from Chinese toon leaves. Background technique [0002] Chinese toon (Toona sinensis), also known as Chinese toon tree and Chinese toon head, is a tall deciduous tree belonging to the family Meliaceae. It is named for its special fragrance of its tender buds. Chinese toon is widely distributed in my country, from Liaoning in the east, to Gansu in the west, to Guangdong, Guangxi, Yunnan in the south, and to southern Inner Mongolia in the north. Among them, Anhui, Henan, Shandong and other provinces are more cultivated. Chinese toon, as an excellent woody vegetable, is widely loved by people because of its advantages such as growth speed, strong ecological adaptability, and high nutritional value, and people's demand for Chinese toon is also increasing day by day. [0003] At present, in terms of production, because Chinese toon has been prop...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 侯金艳吴丽芳
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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