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A kind of method for rapidly propagating wintersweet plant

A technology of wintersweet and plants, applied in the field of rapid propagation of wintersweet plants, can solve the problems of high cost, low reproduction coefficient, long cycle, etc., and achieve the effect of fast regeneration speed, high reproduction coefficient and simple operation

Active Publication Date: 2021-08-24
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, in terms of production, the breeding of wintersweet mainly adopts traditional methods such as sowing, grafting and branching, but these conventional breeding methods have cumbersome reproduction processes, low reproduction coefficients, and are limited by seasons, which are far from satisfying the market demand for wintersweet species. Demand for Seedlings and Rapid Development of Wintersweet Industry
At present, although the research on the propagation of wintersweet by cutting technology has been reported, there are generally problems such as limited propagation materials, difficulty in rooting, low rooting efficiency and seasonal restrictions on propagation.
In addition, we used plant tissue culture technology to establish an in vitro regeneration system for wintersweet plants, but there are problems such as cumbersome reproduction process, long cycle and high cost in the reproduction process.

Method used

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  • A kind of method for rapidly propagating wintersweet plant
  • A kind of method for rapidly propagating wintersweet plant
  • A kind of method for rapidly propagating wintersweet plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for rapid propagation of wintersweet plants, comprising the following steps:

[0029] (1) Take the winter plum strain tissue culture seedlings of the size of 3 months as the source of explants, cut the stem section into a section with 2 axillary buds and 2 leaves, and set aside; Cultivated seedlings are obtained by patent CN201710305050.4, a method for efficient in vitro regeneration of wintersweet plants;

[0030] (2) After the stem segment in step (1) is washed with running water, after 1 min of water-soluble disinfection with 0.01% carbendazim and 0.01% potassium permanganate added, the residual solution on the surface of the stem segment is dried for later use;

[0031] (3) The sterilized stem section in step (2) is subjected to adventitious root induction treatment, that is, the morphological lower end of the stem section is soaked in 5 mg / L indole acetic acid, 20 mg / L melatonin, 100 mg / L indole butyl acid, 0.01mg / L VB12, 0.3‰ Tween-20 in the rooting prom...

Embodiment 2

[0036] A method for rapid propagation of wintersweet plants, comprising the following steps:

[0037] (1) With seedling age being 12 months the winter plum strain tissue culture seedling of size is explant source, the cutting section that grows 2.0cm with stem segment, has 2 axillary buds and 2 blades is standby; Wherein tissue culture The seedlings are obtained through the patent CN201710305050.4, a method for efficient in vitro regeneration of wintersweet plants;

[0038] (2) After the stem section in the step (1) is washed with running water, after 2 minutes of water-soluble disinfection with the addition of 0.02% carbendazim and 0.05% potassium permanganate, the residual solution on the surface of the stem section is dried for subsequent use. Segment cut as shown in figure 1 shown;

[0039] (3) The sterilized stem section in step (2) is subjected to adventitious root induction treatment, that is, the morphological lower end of the stem section is soaked in 7.5mg / L indole...

Embodiment 3

[0044] A method for rapid propagation of wintersweet plants, comprising the following steps:

[0045] (1) With the 1-2 year old wintersweet seed seedling as the source of explants, the stem section is cut to grow 3.0cm, the section with 2 axillary buds and 2 blades, for subsequent use;

[0046] (2) After the stem section in the step (1) is washed with running water, after 3 minutes of water-soluble disinfection with 0.05% carbendazim and 0.2% potassium permanganate, the residual solution on the surface of the stem section is dried for subsequent use;

[0047](3) The sterilized stem section in step (2) is subjected to adventitious root induction treatment, that is, the morphological lower end of the stem section is soaked in 10mg / L indole acetic acid, 100mg / L melatonin, 300mg / L indole butyl acid, 0.5mg / L VB12, 0.6‰ Tween-20 in the rooting promotion solution, the adventitious root induction treatment was carried out, and the treatment time was 20s;

[0048] (4) the morphologica...

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Abstract

The invention discloses a method for rapidly propagating wintersweet plants, and relates to the technical field of rapid plant propagation. The invention uses the tissue-cultured regenerated seedlings of wintersweet excellent strains or the stem sections of seed seedlings as explants, undergoes surface disinfection, induces adventitious roots, and colonizes And seedling management and other steps to finally obtain complete regenerated plants. The beneficial effects of the present invention are: simple operation, low cost; few materials, abundant materials; fast propagation speed, high reproduction coefficient and no seasonal restrictions on reproduction, etc. is of great significance.

Description

technical field [0001] The invention relates to the technical field of rapid propagation of plants, in particular to a method for rapid propagation of wintersweet plants. Background technique [0002] Wintersweet (Chimonanthus praecox (Linn.) Link), also known as wintersweet, blooms in the cold winter, yellow as wax, fragrant, beautiful in shape and long flowering period. It is a precious flower and tree unique to my country and a world-renowned ornamental tree species in gardens. and cut flower species. Due to the pleasant fragrance of wintersweet flowers, the essence components extracted from wintersweet flowers are more abundant than rose oil and jasmine essence. The price of wintersweet aroma oil is much higher than rose oil and jasmine aroma oil in the international market. The roots, stems, leaves, flower buds, and fruits of wintersweet are all human medicines, which have the effects of relieving summer heat, promoting body fluid, smoothing qi and relieving cough. In ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G17/00A01G2/10
CPCA01G17/005A01H4/001A01H4/008A01G2/10
Inventor 侯金艳吴丽芳陈雪王萍
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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