Acid flavor type tobacco Chimonanthus Praecox flavor and its preparation method
A technology of wintersweet flowers and spices, which is applied in the fields of essential oils/flavors, preparation of tobacco, tobacco, etc., and can solve the problems of unreported research on spices for tobacco
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Embodiment 1
[0018] The present invention comprises the following steps:
[0019] (1), the wintersweet flower and water are refluxed and extracted for 2 hours in a mass ratio of 1:5 to obtain the extract;
[0020] (2), adding the wintersweet flower extract mass percentage is 2% glucose, 20% bran and 0.05% sodium chloride to the wintersweet flower extract to prepare the culture medium;
[0021] (3), mix 10g peptone, 60g beef extract, 0.9g ammonium sulfate, 0.3g sodium sulfate heptahydrate, 0.5g sodium chloride, 30g glucose, 0.1g ferrous sulfate heptahydrate, 30g calcium carbonate and 1L water, Prepare seed culture medium;
[0022] (4), Clostridium butyricum CB-2 is inoculated in the seed culture medium, makes its bacterium concentration reach 0.1 * 10 10 Individual / ml, make the clostridium butyricum CB-2 solution of fragrant-producing bacterium, under aseptic condition, inoculate the clostridium butyricum CB-2 solution that mass percent is 0.1%;
[0023] (5) Anaerobic fermentation for 2 ...
Embodiment 2
[0026] The present invention comprises the following steps:
[0027] (1), wintersweet flower and water are refluxed extraction 4h in the ratio of mass ratio 1: 20, obtain extract;
[0028] (2), adding the wintersweet flower extract mass percentage is 8% glucose, 20% bran and 0.75% sodium chloride in the wintersweet flower extract, makes the culture medium;
[0029] (3), mix 10g peptone, 60g beef extract, 0.9g ammonium sulfate, 0.3g sodium sulfate heptahydrate, 0.5g sodium chloride, 30g glucose, 0.1g ferrous sulfate heptahydrate, 30g calcium carbonate and 1L water, Prepare seed culture medium;
[0030] (4), Clostridium butyricum CB-2 is inoculated in the seed culture medium, makes its bacterium concentration reach 1.5 * 10 10 Individual / ml, make the clostridium butyricum CB-2 solution of fragrant-producing bacterium, under aseptic condition, inoculate the clostridium butyricum CB-2 solution that mass percent is 2%;
[0031] (5) Anaerobic fermentation for 10 days at a pH valu...
Embodiment 3
[0034] The present invention comprises the following steps:
[0035] (1), wintersweet flower and water are refluxed extraction 3h by the ratio of mass ratio 1: 10, obtain extract;
[0036] (2), adding wintersweet flower extract mass percent to be 5% glucose, 20% bran and 0.35% sodium chloride in the wintersweet flower extract, make culture medium;
[0037] (3), mix 10g peptone, 60g beef extract, 0.9g ammonium sulfate, 0.3g sodium sulfate heptahydrate, 0.5g sodium chloride, 30g glucose, 0.1g ferrous sulfate heptahydrate, 30g calcium carbonate and 1L water, Prepare seed culture medium;
[0038] (4), Clostridium butyricum CB-2 is inoculated in the seed culture medium, makes its bacterium concentration reach 1.0 * 10 10 pcs / ml to prepare the aroma-producing bacterium Clostridium butyricum CB-2 solution, under aseptic conditions, inoculate the Clostridium butyricum CB-2 solution with a mass percentage of 1%;
[0039] (5) Anaerobic fermentation for 6 days at a pH value of 4.0 and...
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