Culture medium and culture method for promoting growth of regeneration buds of echinacea purpurea

A technology for growing medium and regenerating buds, which is applied in the field of plant biology and can solve problems such as poor growth of regenerated buds of Echinacea purpurea

Active Publication Date: 2015-06-17
广州市泰丰源实业有限公司
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention provides a kind of medium that promotes the growth of echinacea regenerated buds in order to overcome the defects of poor growth of echinacea regenerated buds in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Culture medium and culture method for promoting growth of regeneration buds of echinacea purpurea
  • Culture medium and culture method for promoting growth of regeneration buds of echinacea purpurea
  • Culture medium and culture method for promoting growth of regeneration buds of echinacea purpurea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] S1. Preparation of regenerated buds: first obtain the petiole explant of Echinacea purpurea, the petiole explant comes from the diploid aseptic seedling of Echinacea purpurea under tissue culture conditions (the preparation of the explant can refer to the conventional technical method in the art), and then the petiole The explants were inoculated on regeneration medium (regeneration medium containing 30 g / L sucrose, 0.3 mg / L BA, 0.01 mg / L NAA, solidified with 4.5 g / L agar, in 1.4 kg cm -2 Sterilized MS medium for 20 min). When the regenerated shoot grows to about 2 cm high with leaves, the bud is cut off from the explant tissue, which can be used as the material used in the present invention—the regenerated shoot.

[0026] S2. Cultivation of regenerated shoots: cut out the shoots obtained from the diploid Echinacea purpurea petiole explants in step S1, and inoculate them in the culture medium supplemented with different concentrations of DA-6 (0, 0.08, 0.16, 0.32 mg / L) ...

Embodiment 2

[0032] S1. Preparation of regenerated buds: first obtain the petiole explants of Echinacea purpurea, and the petiole explants are from triploid aseptic seedlings of Echinacea purpurea under tissue culture conditions (the preparation of explants can refer to the conventional technical methods in the art), and then the petiole The explants were inoculated on the regeneration medium (the regeneration medium contained 30 g / L sucrose, 0.3 mg / L BA, 0.01 mg / L NAA, solidified with 4.5 g / L agar, in 1.4 kg cm -2 Sterilized MS medium for 20 min). When the regenerated shoot grows to about 2 cm high with leaves, the bud is cut off from the explant tissue, which can be used as the material used in the present invention—the regenerated shoot.

[0033] S2. Cultivation of regenerated shoots: cut out the shoots obtained from the petiole explants of triploid Echinacea purpurea described in step S1, and inoculate them with different concentrations of DA-6 (0, 0.08, 0.16, 0.32 mg / L) On th...

Embodiment 3

[0038] S1. Preparation of regenerated buds: firstly obtain the petiole explants of Echinacea purpurea, the petiole explants are from the tetraploid aseptic seedlings of Echinacea purpurea under tissue culture conditions (the preparation of explants can refer to the conventional technical method in the art), and then the petiole The explants were inoculated on regeneration medium (regeneration medium containing 30 g / L sucrose, 0.3 mg / L BA, 0.01 mg / L NAA, solidified with 4.5 g / L agar, in 1.4 kg cm -2 Sterilized MS medium for 20 min). When the regenerated shoot grows to about 2 cm high with leaves, the bud is cut off from the explant tissue, which can be used as the material used in the present invention—the regenerated shoot.

[0039] S2. Cultivation of regenerated shoots: cut out the shoots obtained from the petiole explants of tetraploid Echinacea purpurea described in step S1, and inoculate them into the plants supplemented with different concentrations of DA-6 (0, 0.08, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the field of plant biotechnology, and specifically discloses a culture medium and a cultivation method for promoting the growth of echinacea purpurea regenerated buds. In addition to adding 15-60g / L sucrose, 3-9g / L agar and 0.01-0.2mg / LNAA to the MS formula, the culture medium also added diethylaminoethanol hexanoate according to the regenerated shoots of different ploidy. (DA-6) sensitivity is different, especially adding the corresponding concentration of DA-6. The culture medium can significantly improve the height and weight of regenerated shoots, the weight, diameter and quantity of roots, the length of primary roots, the total length of roots, the ratio of root to shoot and the survival rate of transplanting. The application of the invention will promote the development of scientific research related to the echinacea purpurea biotechnology breeding.

Description

technical field [0001] The invention relates to the field of plant biotechnology, in particular to a culture medium and a culture method for promoting the growth of regenerated buds of Echinacea purpurea. Background technique [0002] Echinacea ( Echinacea purpurea L.) is a perennial herb of Compositae, which has a good immune regulation effect, and its extract is an immune enhancer and immune regulator that has received widespread attention in the world in recent years. Echinacea has become a world-renowned medicinal plant due to its precise efficacy. In order to obtain multiple varieties of Echinacea purpurea, people usually use tissue culture technology for regeneration or regenerative cloning. Regeneration or regenerative cloning of plants using tissue culture techniques usually involves two steps. The first step is to induce adventitious shoots from explants. The second step is to grow the regenerated shoots into complete plants ready for transplanting. For Echina...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 陈晓鹭杨跃生吴鸿李栋梁
Owner 广州市泰丰源实业有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap