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Method for cultivating curly pondweed embryo and curly pondweed karren tissue

A technology of embryo culture and weed grass, which is applied in the field of plant tissue culture, can solve the problems of low germination rate, difficulty in germination, difficulty in large-scale propagation and promotion, etc.

Inactive Publication Date: 2004-11-17
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the seeds are extremely difficult to germinate under natural conditions, the growth of stone buds is limited by seasons and the germination rate is low, it is difficult to propagate on a large scale.
[0004] At present, there is no relevant report on the research on the tissue culture technology of Smilax at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0034] 1. Establish a sterile system

[0035] Wash the seeds and stone buds of Smilax chinensis with tap water, soak them in 70% ethanol for 30 seconds, sterilize them with 0.1% HgClz aqueous solution containing a small amount of Tween 80 for 9 minutes, and wash them with sterile distilled water for 5 times. Inoculate the sterilized seeds and stone buds on the MS medium without exogenous hormones supplemented with 3% sucrose, and establish sterile conditions at a temperature of 25±2°C and a light intensity of 2000lx and a daily light of 12h. system.

[0036] 2. The implementation mode of the method for cultivating smilax embryos

[0037] (1) Callus induction and subculture

[0038] In the ultra-clean workbench, the aseptic Smilax seeds were subjected to embryo stripping, and they were placed on 2,4-D 0.5 mg / L, IAA 1.0 mg / L medium to induce callus. The temperature is 25±2°C, the light intensity is 800 lx, and the light is 12 hours a day. The callus induction culture is carr...

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PUM

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Abstract

The invention relates to the field of plant tissue culture technology, wherein curly pondweed embryo cultivation technique and karren fast breeding method are employed to realize curly pondweed high grade sprout cultivation by means of mass production, so as to recover the fine water ecological condition.

Description

1. Technical field [0001] The invention belongs to the technical field of plant tissue culture. 2. Background technology [0002] The pollution of my country's water ecosystem is serious, and the decline of water environment quality has caused serious damage to aquatic biological resources. It is of great significance to restore the balance of aquatic ecosystem, purify water quality, promote the development of fishery, and introduce water purification plants. [0003] Potamogeton crispus L. is a perennial submerged herbaceous plant of Potamogetonaceae, which has high nutritional value and economic value. Smilax has strong adaptability to water eutrophication, and can also grow densely in water bodies seriously polluted by domestic sewage. It has the function of purifying water bodies and is the main aquatic plant for purifying water quality from winter to early summer. However, because the seeds are extremely difficult to germinate under natural conditions, the growth of st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H3/04A01H4/00A23K10/30A23K50/75C02F3/32
CPCY02W10/37
Inventor 安树青郑丽屏蒋金辉黄成姚鑫
Owner NANJING UNIV
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