Method for extracting histocytes from large Michelia alba lateral buds

A technology of tissue cells and white orchids, applied in the field of tissue cell extraction of lateral buds of big white orchids, to achieve the effects of reducing heat loss, high yield, reducing processing time and solvent usage

Inactive Publication Date: 2018-09-18
上海数儒节能科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, traditional water or alcohol extraction is generally used for buds, and there is no further extraction by other techniques

Method used

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Experimental program
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Embodiment Construction

[0031] A method for extracting lateral bud tissue cells of orchid japonica, comprising the steps of:

[0032] Step 1, plant cultivation: take the seeds of the Great White Orchid V3 variety and put them into the culture medium and apply sufficient nutrients to carry out plant cultivation;

[0033] Step 2, sprout cultivation: wait for the plant of Dabai Orchid V3 variety to bloom, intercept the stem of the plant, and cut the stem of the intercepted plant into segments for sprout cultivation in units of nodes;

[0034] Step 3, lateral bud interception: get the lateral bud part of new bud and cut leaves;

[0035] Step 4, proliferation and growth of lateral buds: multiply and grow the cut leaves of lateral buds obtained from new shoots;

[0036] Step 5. Extraction of embryonic tissue at the growth point of the lateral buds: cut the cell tissue of the growth point of the lateral buds into small pieces, cover and soak them with 5 times the amount of pure water, and put them into an ...

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Abstract

The invention discloses a method for extracting histocytes from large Michelia alba lateral buds, and relates to the technical field of biological histocyte extraction. The method comprises the following steps: 1, plant culturing; 2, bud cultivation; 3, lateral bud cutting; 4, lateral bud propagation growth; 5, lateral bud growing point embryonic tissue extraction; 6, extraction and purification;and 7, extraction component analysis. Large Michelia alba V3 variety flowered plant segment stalk buds undergo tissue culture, and newborn cells cut from the lateral buds undergo high frequency extraction at 50-60 DEG C in an ultrasonic device with pure water as a solvent.

Description

technical field [0001] The invention belongs to the technical field of biological tissue cell extraction, in particular to a method for extracting tissue cells from lateral buds of orchids. Background technique [0002] General tissue culture all takes the terminal bud with meristem ability to cultivate, and the growth point of stem top adopts bud proliferation (bud long bud) or induces pseudoprotosphere (PLBs) meristematic seedling mode more, wherein bud proliferation (bud Bud growth), that is, to reduce or remove the dominance of the terminal buds, so that the side buds are easy to grow, and the buds are multiplied in this way. At present, traditional water or alcohol extraction is generally used for buds, and there is no further extraction by other techniques. Contents of the invention [0003] The purpose of the present invention is to provide a method for extracting tissue cells from the lateral buds of Dabai orchid, by taking the flowering plant stem buds of Dabai o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00B01D11/02
CPCA01H4/001A01H4/008B01D11/0265
Inventor 萧宏颖
Owner 上海数儒节能科技有限公司
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