Method for tissue culture of pigment marigold

Abstract

The invention relate to a method for tissue culture of a pigment marigold in the field of biotechnology, which comprises the following steps: sterilizing marigold leaves and inducing the same, wherein a differential medium used in the induction is specifically MS basic culture medium, an additive comprises 3 mg / L 6-BA, 3 mg / L IAA, 30 g / L sucrose and 6 g / L plant gel, and the culture condition comprises that the temperature is between 23 and 27 DEG C, the illumination time is between 10 and 20 hours per day, the illumination intensity is between 2,000 and 3,000 lux, and the induction time of an adventitious bud is between 9 and 14 days; performing secondary culture on the deferential adventitious bud, wherein the induction bud growth medium is same as the differential medium in the step one, and the culture condition comprises that the temperature is between 23 and 27 DEG C, the illumination time is between 10 and 20 hours per day, the illumination intensity is between 2,000 and 3,000 lux, and the culture time is between 30 and 50 days; and performing rooting culture on the deferential stretched-out bud for 30 days to obtain a marigold regeneration plant. The method has the advantages that materials are convenient to obtain, a large amount of marigold regeneration plants can be induced at a high frequency, the survival rate of the regeneration plants is high, the variation of the regeneration rate is small, and the genetic stability is higher.

Description

Technical field [0001] The invention relates to a tissue culture method in the field of biotechnology, in particular to a tissue culture method of pigment marigold. Background technique [0002] Tagetes erecta, also known as smelly hibiscus, is a species of marigold in the Asteraceae family. It is native to Mexico and the Americas and is now cultivated all over the world. It can be divided into ornamental marigold and pigment marigold according to its use. The lutein content in the petals of pigment marigold is extremely rich. It is an ideal raw material for extracting natural lutein. Lutein can be widely used in food coloring, medicine and poultry. In feed, it has high economic value. [0003] In recent years, great progress has been made in the selection and breeding of foreign marigold varieties. However, the breeding of F1 generation marigold in China is still in its infancy. Some F1 seed production technologies such as seeding technology, seedling raising technology, parent ...

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Problems solved by technology

[0008] Pablo E, Vanegas etc. published "Plant regeneration via organogenesis in marigold" (plant regeneration via organogenesis in marigold) in "Plant Cell Tissue and Organ Culture" (plant cell tissue and organ culture) 2002 69 phase 279~283 pages ) article, it is mentioned in the text that the regeneration system has been established for explants using marigold leaves, but there is a shortcoming (69.3%) of low adventitious bud induction rate, and it is also found that under its conditions to induce plant regeneration, adventitious buds still exist Tendency to vitrification, unable to develop into normal seedlings

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