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Ceratophyllum demersum L. tissue culture and rapid propagation method

A technology of tissue culture and hornwort, applied in the field of plant tissue culture, can solve problems such as pollution and limited provenance, and achieve the effect of easy method, convenient operation and high yield

Inactive Publication Date: 2016-06-08
SHUISHENGZAOAN BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is still a lack of mature technology, which can multiply high-quality engineering seedlings of hornwort on a large scale, and at the same time overcome the problem of limited provenance and serious pollution of this species in the natural environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A. Selection and pretreatment of explants: Select stems that grow vigorously, are bright green in color, and have no pests and diseases that can germinate axillary buds as explants, soak them in alkaline detergent for 30 minutes, rinse them under tap water for two hours, and rinse them with distilled water Rinse 4-5 times repeatedly, wash again 4-5 times with sterile water, and place on a sterile operating table;

[0022] B. Sterilization of explants: Soak in 75% ethanol for 15 seconds on the aseptic operating table, rinse with sterile water 4-5 times, and then use 5% plant tissue culture antibacterial agent (PPM) to disinfect the surface for 5 minutes , rinse with sterile water 4-5 times, blot the water dry on sterile filter paper, and set aside;

[0023] C. Bud induction: select 0.50mg / L6-BA and 1.30mg / LIAA MS solid medium, the sucrose concentration is 3% (mass volume ratio), the pH is adjusted to 5.8, and the medium is packed in a 50 ml Erlenmeyer flask. bottle of 2...

Embodiment 2

[0050] A. Selection and pretreatment of explants: Select stems that grow vigorously, are bright green in color, and have no pests and diseases that can germinate axillary buds as explants, soak them in alkaline detergent for 30 minutes, rinse them under tap water for two hours, and rinse them with distilled water Rinse 4-5 times repeatedly, wash again 4-5 times with sterile water, and place on a sterile operating table;

[0051] B. Sterilization of explants: Soak in 75% ethanol for 15 seconds on the aseptic operating table, rinse with sterile water 4-5 times, and then use 5% plant tissue culture antibacterial agent (PPM) to disinfect the surface for 5 minutes , washed with sterile water for 4-5 times, blotted dry on sterile filter paper, and set aside;

[0052]C. Bud induction: select 0.50mg / L6-BA and 1.30 / LIAA MS solid medium, the sucrose concentration is 3% (mass volume ratio), the pH is adjusted to 6.0, and the medium is packed in a 50 ml Erlenmeyer flask, each bottle 20 m...

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Abstract

The invention discloses a ceratophyllum demersum L. tissue culture and rapid propagation method. The ceratophyllum demersum L. tissue culture and rapid propagation method includes A, selecting explants, namely, selecting robust clear green plant stem segments without diseases and insect pests as explants; B, sterilizing the explants, namely, treating the explants by alcohol and plant tissue germicide to prepare germfree explants; C, inducing buds, namely, selecting hormone types and hormone concentration combination to induce bud growth; D, performing propagation culture, namely, adjusting hormone combination for propagation culture; E, hardening seedlings and transplanting, namely, opening bottle openings in an incubator, culturing for two days and transferring into natural water for culture. The ceratophyllum demersum L. tissue culture and rapid propagation method has the advantages that the method cannot be limited by seasons and environments, and a great number of aseptic seedlings are cultured for aquatic ecosystem restoration.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for tissue culture and rapid propagation of the submerged plant hornwort. The method realizes the mass production of the submerged plant hornwort in the laboratory by utilizing complete aseptic and strict temperature control measures , and can be extended to ecological environment restoration. Background technique [0002] Hornwort belongs to the genus Hornwort of the family Chrysphorus. It is used as a water purification tool species and a pioneer species of vegetation restoration in lake ecological restoration projects, as bait, refuge and spawning place in the process of fish, shrimp and crab pond culture, and in the process of indoor ornamental aquaculture. as scenery material. At present, there is still a lack of mature technology, which can multiply high-quality engineering seedlings of hornwort on a large scale, and at the same time overc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/005
Inventor 不公告发明人
Owner SHUISHENGZAOAN BIOTECH WUHAN CO LTD
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