Induced rapid propagation culture medium for somatic embryos of leaves in vitro of photinia x frasery
A technology of isolated leaves and somatic embryos, applied in the field of botany, can solve the problems of reducing the degree of improved seeding, restricting the development, popularization and application of tree species, serious viruses, etc., achieving the effects of robust seedling growth and solving the problem of germplasm degradation.
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[0046] figure 1 A test-tube plantlet cultivated for value-added according to the present invention; figure 2 Somatic embryos of leaves of Photinia fragrans according to the present invention at different stages; image 3 The adventitious buds formed by the leaf body embryo of Photinia fragrans according to the present invention; Figure 4 Indefinite teeth formed directly by the blades of Photinia fragrans according to the present invention; Figure 5 The clustered buds formed for the multiplication and rapid propagation of the present invention; Image 6 It is the test-tube plantlet after rooting culture and seedling hardening according to the present invention; Figure 7 It is the plant grown through transplanting according to the present invention.
[0047] Such as Figure 1-Figure 7 Shown:
[0048] Rapid propagation medium for in vitro somatic embryo induction of Photinia fragrans, including bud initiation medium, test-tube plantlet proliferation medium, first inducti...
Embodiment 1
[0058] Proliferation medium for test tube seedlings: modified MS + 1.0mgL -1 BA+1.0mgL -1 KT + 0.1mgL -1 NAA + 6.0gL -1 Agar + 30gL -1 Sucrose, and the pH value is 5.8; the first induced somatic embryo and / or adventitious bud formation medium is: modified MS + 0.5mgL -1 2,4-D + 0.5mgL -1 BA+0.5mgL -1 NAA + 3.0gL -1 Phytagel + 20gL -1 Sucrose, and the pH value is 5.8; the second induced somatic embryo and / or adventitious bud formation medium is: modified MS + 0.1mgL -1 2,4-D + 0.5mgL -1 BA+10mgL -1 NAA + 3.0gL -1 Phytagel + 20gL -1 Sucrose, and the pH value is 5.8; the third induced somatic embryo and / or adventitious bud formation medium is: modified MS + 2.0mgL -1 NAA+0.2mgL -1 2,4-D + 0.5mgL -1 BA+ 0.5 mgL -1 KT+ 6.0gL -1 Agar + 30gL -1 Sucrose, and the pH value is 5.8; the first somatic embryo and / or adventitious bud growth and differentiation medium is: modified MS + 2.0mg L -1 BA + 3.0gL -1 Phytagel + 20gL -1 Sucrose, and the pH value is 5.8; the secon...
Embodiment 2
[0060] Proliferation medium for test tube seedlings: modified MS + 1.5mgL -1 BA+1.5mgL -1 KT + 0.3mgL -1 NAA + 6.5gL -1 Agar + 30gL -1 Sucrose, and the pH value is 5.9; the first induced somatic embryo and / or adventitious bud formation medium is: modified MS + 0.5mgL -1 2,4-D + 0.7mgL -1 BA+1.2mgL -1 NAA + 3.2gL -1 Phytagel + 20gL -1 Sucrose, and the pH value is 5.9; the second induced somatic embryo and / or adventitious bud formation medium is: modified MS + 0.1mgL -1 2,4-D + 0.5mgL -1 BA+20mgL -1 NAA + 3.2gL -1 Phytagel + 20gL -1 Sucrose, and the pH value is 5.9; the third induced somatic embryo and / or adventitious bud formation medium is: modified MS + 3.0mgL -1 NAA+0.4mgL -1 2,4-D + 1.0mgL -1 BA+ 1.2mgL -1 KT+ 6.7gL -1 Agar + 30gL -1 Sucrose, and the pH value is 5.9; the first somatic embryo and / or adventitious bud growth and differentiation medium is: modified MS + 3.0mg L -1 BA + 3.3gL -1 Phytagel + 20gL -1 Sucrose, and the pH value is 5.9; the second...
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