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Hyacinthus orientalis L. in-vitro rapid propagation method

A hyacinth, in vitro technology, applied in the field of hyacinth in vitro rapid propagation, can solve the problems of increased workload, bulb infection, repeated pollution, etc., and achieve the effect of increased regeneration efficiency, shortened regeneration cycle, and increased induction rate

Inactive Publication Date: 2012-06-27
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is relatively convenient to use bulbs as explants for regeneration, but due to the serious bacterial contamination of bulbs, it often leads to incomplete disinfection, repeated pollution during the cultivation process is very serious, increasing the workload, and the regeneration efficiency of scales as explants is low. Not very ideal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Select an excellent hyacinth variety, Gipsy Queen, and plant it in the field around November of that year, and dig out the bulbs in mid-January of the following year;

[0021] Take young petals as explants for bud induction culture, the medium is MS+BA1.0mg / L+NAA0.5mg / L, pH 5.8, sucrose 30g / L, agar 5g / L. The culture conditions are: temperature 25°C, light: 16 light periods / 8 dark periods. The germination rate can reach 70%, and the average number of buds induced by each explant is about 8.

[0022] After 40 days, a large number of bud clusters appeared on the petals. After cutting the bud clusters, put them into the bud elongation medium for cultivation. The medium is MS+NAA0.5mg / L, pH5.8, sucrose 30g / L, agar 5g / L L. The cultivation conditions were the same as above.

[0023] After 30 days, the buds elongate to 2-4cm, cut the bud clusters, and put them into the rooting medium for cultivation. The rooting medium is MS+NAA0.5mg / L, pH 5.8, sucrose 30g / L, and agar 5g / L. ...

Embodiment 2

[0025] Select an excellent hyacinth variety, Gipsy Queen, and plant it in the field around November of that year, and dig out the bulbs in mid-January of the following year;

[0026] Take young petals as explants for bud induction culture, the medium is MS+BA2.0mg / L+NAA1.0mg / L, pH 5.8, sucrose 30g / L, agar 5g / L. The culture conditions are: temperature 25°C, light: 16 light periods / 8 dark periods. The germination rate can reach 100%, and the average number of buds induced by each explant is about 20.

[0027] After 30 days, a large number of bud clusters appeared on the petals. After the bud clusters were cut, they were placed in the bud elongation medium for cultivation. The medium was MS+BA0.5mg / L+NAA1.0mg / L, pH5.8, sucrose 30g / L, agar 5g / L. The cultivation conditions were the same as above.

[0028] After 20 days, when the buds grow to 2-4cm, cut the bud clusters and put them into the rooting medium for cultivation. The rooting medium is MS+NAA0.5mg / L+activated carbon 1.0...

Embodiment 3

[0030] Select an excellent hyacinth variety, Gipsy Queen, and plant it in the field around November of that year, and dig out the bulbs in mid-January of the following year;

[0031] Take young petals as explants for bud induction culture, the medium is MS+BA3.0mg / L+NAA2.0mg / L, pH 5.8, sucrose 30g / L, agar 5g / L. The culture conditions are: temperature 25°C, light: 16 light periods / 8 dark periods. The germination rate can reach 80%, and the average number of buds induced by each explant is about 12.

[0032] After 35 days, a large number of bud clusters appeared on the petals. After the bud clusters were cut, they were placed in a bud elongation medium for cultivation. The medium was MS+NAA1.0mg / L, pH5.8, sucrose 30g / L, agar 5g / L L. The cultivation conditions were the same as above.

[0033] After 25 days, when the buds grow to 2-4cm, cut the bud clusters and put them into the rooting medium for cultivation. The rooting medium is MS+NAA0.5mg / L+activated carbon 2.0g / L, pH 5.8,...

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Abstract

The invention relates to a hyacinthus orientalis L. in-vitro rapid propagation method, and belongs to the field of biotechnology. The hyacinthus orientalis L. in-vitro rapid propagation method comprises the following steps of 1, selecting hyacinthus orientalis L. seed bulbs growing in an open field for 2 to 3 months, carrying out disinfection, and removing outer scales, 2, taking floret petals, flatwise putting the floret petals on a MS solid medium, and carrying out adventitious bud growth induction culture to obtain tissue blocks containing adventitious buds, 3, longitudinally dividing the tissue blocks containing adventitious buds, and carrying out bud elongation culture by a bud elongation medium, and 4, when length values of the adventitious buds obtained by the step 3 are in a range of 2 to 4 centimeters, cutting the adventitious buds, putting the cut adventitious buds into a rooting medium, and carrying out rooting induction culture to obtain hyacinthus orientalis L. seedlings. The young floret petals of hyacinthus orientalis L. are utilized as explants and directly regenerate a mass of adventitious buds and the adventitious buds further develop into regenerated seedlings. Compared with the existing tissue culture method utilizing scales as explants, the hyacinthus orientalis L. in-vitro rapid propagation method has high regeneration efficiency, a high budding rate of about 100% and a large average amount of buds regenerated by induction of each one explant, wherein the large average amount is about 20.

Description

technical field [0001] The invention relates to a rapid propagation method in the field of biotechnology, in particular to a hyacinth rapid propagation method in vitro. Background technique [0002] Hyacinth (Hyacinthus orientalis L.) is a perennial herb of the Liliaceae Hyacinth genus, native to the Mediterranean Sea and South Africa, and is a very ornamental spring bulb flower widely used all over the world. my country introduced plantation from the Netherlands in the 1980s, but the traditional propagation method takes a long time and has low reproduction efficiency. At the same time, the bulbs are expensive and cannot meet the market demand, which limits the cultivation of hyacinth in China. Therefore, the rapid propagation of hyacinth through tissue culture is an effective technical means, which is conducive to increasing the promotion of hyacinth in my country. [0003] Hyacinth scales are the most commonly used tissue culture material for rapid propagation. Studies h...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 马晓红史益敏董易程鹏
Owner SHANGHAI JIAO TONG UNIV
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