Hyacinthus orientalis L. in-vitro rapid propagation method

A hyacinth, in vitro technology, applied in the field of hyacinth in vitro rapid propagation, can solve the problems of increased workload, bulb infection, repeated pollution, etc., and achieve the effect of increased regeneration efficiency, shortened regeneration cycle, and increased induction rate

Inactive Publication Date: 2012-06-27
SHANGHAI JIAO TONG UNIV
View PDF0 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is relatively convenient to use bulbs as explants for regeneration, but due to the serious bacterial contamination of bulbs, it often leads to incomplete disinfecti...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Select an excellent hyacinth variety, Gipsy Queen, and plant it in the field around November of that year, and dig out the bulbs in mid-January of the following year;

[0021] Take young petals as explants for bud induction culture, the medium is MS+BA1.0mg / L+NAA0.5mg / L, pH 5.8, sucrose 30g / L, agar 5g / L. The culture conditions are: temperature 25°C, light: 16 light periods / 8 dark periods. The germination rate can reach 70%, and the average number of buds induced by each explant is about 8.

[0022] After 40 days, a large number of bud clusters appeared on the petals. After cutting the bud clusters, put them into the bud elongation medium for cultivation. The medium is MS+NAA0.5mg / L, pH5.8, sucrose 30g / L, agar 5g / L L. The cultivation conditions were the same as above.

[0023] After 30 days, the buds elongate to 2-4cm, cut the bud clusters, and put them into the rooting medium for cultivation. The rooting medium is MS+NAA0.5mg / L, pH 5.8, sucrose 30g / L, and agar 5g / L. ...

Embodiment 2

[0025] Select an excellent hyacinth variety, Gipsy Queen, and plant it in the field around November of that year, and dig out the bulbs in mid-January of the following year;

[0026] Take young petals as explants for bud induction culture, the medium is MS+BA2.0mg / L+NAA1.0mg / L, pH 5.8, sucrose 30g / L, agar 5g / L. The culture conditions are: temperature 25°C, light: 16 light periods / 8 dark periods. The germination rate can reach 100%, and the average number of buds induced by each explant is about 20.

[0027] After 30 days, a large number of bud clusters appeared on the petals. After the bud clusters were cut, they were placed in the bud elongation medium for cultivation. The medium was MS+BA0.5mg / L+NAA1.0mg / L, pH5.8, sucrose 30g / L, agar 5g / L. The cultivation conditions were the same as above.

[0028] After 20 days, when the buds grow to 2-4cm, cut the bud clusters and put them into the rooting medium for cultivation. The rooting medium is MS+NAA0.5mg / L+activated carbon 1.0...

Embodiment 3

[0030] Select an excellent hyacinth variety, Gipsy Queen, and plant it in the field around November of that year, and dig out the bulbs in mid-January of the following year;

[0031] Take young petals as explants for bud induction culture, the medium is MS+BA3.0mg / L+NAA2.0mg / L, pH 5.8, sucrose 30g / L, agar 5g / L. The culture conditions are: temperature 25°C, light: 16 light periods / 8 dark periods. The germination rate can reach 80%, and the average number of buds induced by each explant is about 12.

[0032] After 35 days, a large number of bud clusters appeared on the petals. After the bud clusters were cut, they were placed in a bud elongation medium for cultivation. The medium was MS+NAA1.0mg / L, pH5.8, sucrose 30g / L, agar 5g / L L. The cultivation conditions were the same as above.

[0033] After 25 days, when the buds grow to 2-4cm, cut the bud clusters and put them into the rooting medium for cultivation. The rooting medium is MS+NAA0.5mg / L+activated carbon 2.0g / L, pH 5.8,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a hyacinthus orientalis L. in-vitro rapid propagation method, and belongs to the field of biotechnology. The hyacinthus orientalis L. in-vitro rapid propagation method comprises the following steps of 1, selecting hyacinthus orientalis L. seed bulbs growing in an open field for 2 to 3 months, carrying out disinfection, and removing outer scales, 2, taking floret petals, flatwise putting the floret petals on a MS solid medium, and carrying out adventitious bud growth induction culture to obtain tissue blocks containing adventitious buds, 3, longitudinally dividing the tissue blocks containing adventitious buds, and carrying out bud elongation culture by a bud elongation medium, and 4, when length values of the adventitious buds obtained by the step 3 are in a range of 2 to 4 centimeters, cutting the adventitious buds, putting the cut adventitious buds into a rooting medium, and carrying out rooting induction culture to obtain hyacinthus orientalis L. seedlings. The young floret petals of hyacinthus orientalis L. are utilized as explants and directly regenerate a mass of adventitious buds and the adventitious buds further develop into regenerated seedlings. Compared with the existing tissue culture method utilizing scales as explants, the hyacinthus orientalis L. in-vitro rapid propagation method has high regeneration efficiency, a high budding rate of about 100% and a large average amount of buds regenerated by induction of each one explant, wherein the large average amount is about 20.

Description

technical field [0001] The invention relates to a rapid propagation method in the field of biotechnology, in particular to a hyacinth rapid propagation method in vitro. Background technique [0002] Hyacinth (Hyacinthus orientalis L.) is a perennial herb of the Liliaceae Hyacinth genus, native to the Mediterranean Sea and South Africa, and is a very ornamental spring bulb flower widely used all over the world. my country introduced plantation from the Netherlands in the 1980s, but the traditional propagation method takes a long time and has low reproduction efficiency. At the same time, the bulbs are expensive and cannot meet the market demand, which limits the cultivation of hyacinth in China. Therefore, the rapid propagation of hyacinth through tissue culture is an effective technical means, which is conducive to increasing the promotion of hyacinth in my country. [0003] Hyacinth scales are the most commonly used tissue culture material for rapid propagation. Studies h...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00
Inventor 马晓红史益敏董易程鹏
Owner SHANGHAI JIAO TONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap