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Myriophyllum aquaticum tissue culture and rapid propagation method thereof

A technology for tissue culture of chrysalis pink green, applied in the field of plant tissue culture, can solve the problems of scarcity, chrysalis spicosa not concretely implemented, and changes in the way and conditions of tissue culture, etc., to achieve convenient operation, The method is easy to operate and the effect of high yield

Active Publication Date: 2015-05-13
INST OF AQUATIC LIFE ACAD SINICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are many reports on the tissue culture of foxtail algae, but there is no specific confirmation of what kind of foxtail algae it is except for foxtail algae
Moreover, there are very few reports on the physiology, biochemistry and ecology of P. viridis
Although it has the common characteristics of Foxtail algae, its own characteristics also lead to changes in the way and conditions of tissue culture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A method for tissue culture and rapid propagation of P. viridans, comprising the following steps:

[0046] A. Selection and pretreatment of explants: choose robust growth, bright green color, and no stems (with or without buds) as explants, soak them in alkaline detergent for 30 minutes, and then rinse them under tap water for 2 hours. , rinsed repeatedly 4-5 times with distilled water, and then rinsed repeatedly 4-5 times with sterile water, and placed on a sterile operating table.

[0047] B, explant sterilization: soak 15s with 70% ethanol (volume ratio) at first on the aseptic operation table, rinse 4-5 times with sterile water, then use 0.1% (mass volume ratio, 0.1g mercuric chloride Add mercuric chloride in 100ml water and carry out surface disinfection for 5 min, rinse with sterile water for 4-5 times, put into 0.15% (mass volume ratio) sterile citric acid solution for cutting, dry the water on sterile filter paper, stand-by.

[0048] C. Callus and shoot induct...

Embodiment 2

[0056] A method for tissue culture and rapid propagation of P. viridans, comprising the following steps:

[0057] A, explant selection and pretreatment: selection growth is robust, and color is bright green, and the stem segment (with or without young bud all can) that does not have damage by disease and pest is used as explant, removes young leaf, soaks 30min with alkaline detergent, then in Rinse under tap water for 2 hours, repeatedly rinse with distilled water for 4-5 times, and then repeatedly wash with sterile water for 4-5 times, and place on a sterile operating table.

[0058] B, explant sterilization: first soak 15s with 70% (volume ratio) ethanol on aseptic operating table, rinse 4-5 times with sterile water, carry out surface with 0.1% (mass volume ratio) mercuric chloride again Disinfect for 2 minutes, rinse with sterile water 4-5 times, put into 0.15% (mass volume ratio) sterile citric acid solution for cutting, dry the water on sterile filter paper, and set aside...

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PUM

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Abstract

The invention relates to a myriophyllum aquaticum tissue culture and a rapid propagation method thereof. The method comprises the following steps: A) selecting an explant, namely selecting a plant stem with robust growth, bright green color and no plant diseases or insect pests as the explant; B) sterilzing the explant, namely performing treatment on the explant with alcohol and mercury chloride; C) performing bud induction, namely selecting optimal hormone mixture ratio to induce bud growth; D) performing proliferation culture, namely adjusting the hormone mixture ratio and the concentration of a culture medium to perform proliferation culture; E) rooting, namely selecting an appropriate cell auxin to perform rooting induction; and F) hardening and transplanting, namely opening a bottle mouth in a culture box, culturing for two days and then transferring into a natural water body for culture. The technology can not subject to seasonal and environmental restrictions, and a large number of sterile seedlings can be cultured for restoring an aquatic ecosystem.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for tissue culture and rapid propagation of the submerged plant Prunus viridans. The method utilizes complete aseptic and strict temperature control measures to realize a large number of submerged plants in the laboratory. production, and can be extended to ecological environment restoration. Background technique [0002] P. viridis belongs to the family Angiospermae, Dicotyledoneae, and Haloragidaceae. At present, it is urgently needed as a water purification tool species and vegetation restoration pioneer species in lake ecological restoration projects, as bait, refuge and spawning places in fish, shrimp and crab pond breeding, and as scenery materials in indoor ornamental aquarium breeding. Use tissue culture technology to obtain and expand its high-quality engineering seedlings on a large scale to overcome the problem of limited provenance a...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 李涛任明磊李琪母丹丹赵进东
Owner INST OF AQUATIC LIFE ACAD SINICA
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