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Method for quickly reproducing in-vitro pea nut

An isolated and rapid technology, applied in the field of plant tissue culture, can solve the problems of unfavorable large-scale promotion, poor culture effect, complicated operation, etc., and achieve the effect of promoting high-quality growth, low cost, and simple operation

Inactive Publication Date: 2009-04-22
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] In the existing in vitro cultivation technology of peanuts, the concentration of plant growth substances is relatively high, and some require special equipment, which is costly, complicated to operate, and the cultivation effect is not very good, which is not conducive to large-scale promotion

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The peanut rapid propagation method in vitro of the present embodiment, its concrete steps are as follows:

[0034] (1) Soak peanut epicotyls with 10 mass % sodium hypochlorite solution at room temperature for 5 minutes, and disinfect the surface;

[0035] (2) Preparation of adventitious bud development medium (add 0.0006mg / mL 4-PU and 0.005mg / mL 6-BA to MS solid medium, pH 6.0) Place the above-mentioned sterilized peanut epicotyl on the adventitious bud development medium In the culture medium, under the conditions of 25±2°C and light intensity of 2000 lux, static culture for 9 days;

[0036] (3) Prepare shoot elongation medium (add 0.003mg / mL gibberellic acid GA3 in MS solid medium, pH 6.0), place the epicotyl after germination in this shoot elongation medium, and in 25 Cultivate for 12 days under the conditions of ±2°C and light intensity of 2000 lux;

[0037] (4) Rooting and culturing to obtain seedlings.

[0038] Taking peanut epicotyls cultured on MS solid medi...

Embodiment 2

[0040] The peanut rapid propagation method in vitro of the present embodiment, its concrete steps are as follows:

[0041](1) Peanut cotyledons were soaked at room temperature for 8 minutes with 11 mass % sodium hypochlorite solution, and the surface was sterilized;

[0042] (2) Preparation of adventitious bud development medium (add 0.0006mg / mL 4-PU and 0.002mg / mL 6-BA to 1 / 2MS medium, pH 6.0), place the above-mentioned sterilized peanut cotyledons in the adventitious bud development culture In the base, cultured for 9 days under the condition of 25±2°C and light intensity of 3000 lux;

[0043] (3) Prepare shoot elongation medium (add 0.003mg / mL 6-BA to MS solid medium, pH 6.0), put the cotyledons after sprouting in the shoot elongation medium, at 25±2 Cultivate for 12 days under the conditions of 2000 lux and light intensity;

[0044] (4) Rooting and culturing to obtain seedlings.

[0045] Peanut cotyledons were cultured in 1 / 2MS solid germination medium without 4-PU, 6-B...

Embodiment 3

[0047] The peanut rapid propagation method in vitro of the present embodiment, its concrete steps are as follows:

[0048] (1) Peanut cotyledons were soaked at room temperature for 5 minutes with 12 mass % sodium hypochlorite solution, and the surface was sterilized;

[0049] (2) Preparation of adventitious bud generation medium (add 0.0002mg / mL 4-PU and 0.005mg / mL 6-BA to B5 medium, pH 6.0) Put the above-mentioned sterilized peanut cotyledons in the adventitious bud generation medium , cultivated for 10 days under the conditions of 25±2°C and light intensity of 3000 lux;

[0050] (3) Prepare shoot elongation medium (add 0.001mg / mL 6-BA in MS solid medium, pH 6.0), place the explant after sprouting in this shoot elongation medium, at 25 Cultivate for 12 days under the conditions of ±2°C and light intensity of 2500 lux;

[0051] (4) Rooting and culturing to obtain seedlings.

[0052] Peanut cotyledons were cultured on MS solid germination medium without 4-PU, 6-BA and other ...

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Abstract

The invention discloses an in vitro rapid propagation method for peanuts. On the basis of the prior tissue culture in vitro peanut propagation which comprises the following steps of (1) disinfecting the surface of an explant, (2) inducing adventitious bud formation, (3) elongating and culturing adventitious buds and (4) performing rooting culture, the method optimizes and improves a culture medium for adventitious bud formation and a culture strip for adventitious bud induction in the step (2), as well as a culture medium for bud elongation and culture conditions in the step (3). The method adopts 4-forchlorfenuron as a main component to promote the adventitious bud formation of plant tissue. Compared with the prior matter for promoting the adventitious bud formation, the 4-forchlorfenuron is low in action concentration and remarkable in effects, and explant tissue close to the surface of the culture medium during adventitious bud formation can not appear black, so as to promote bud growth. Compared with the prior method for promoting adventitious bud elongation, the method of the invention has the advantages that the method does not need to increase special equipment, and is low in the using concentration of gibberellin and cytokinin, low in cost, short in needed time for elongation culture and remarkable in effects.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for rapidly multiplying peanuts in vitro by adopting tissue culture technology. Background technique [0002] Plant tissue culture technology is becoming more and more perfect, and has fully demonstrated its superiority in scientific research and modern agricultural production. It plays a huge role in the detoxification and rapid propagation of agricultural and forestry crops, mutation induction, reformed crop cultivation, cell engineering and genetic engineering breeding, and germplasm preservation. It is a high-tech with broad development potential. The way of plant regeneration is to use isolated organs or tissues for in vitro culture. After dedifferentiation and redifferentiation of cells, adventitious buds are induced. After the adventitious buds gradually elongate and grow leaves to form test-tube plantlets, they are then cut into individual plants and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李玲覃铭刘璨
Owner SOUTH CHINA NORMAL UNIVERSITY
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