Breeding method of yellow bud marker photosensitive male sterile line

A male sterile line and bud yellow technology, which is applied in the fields of botany equipment and methods, application, plant gene improvement, etc., can solve the problems of large-scale application of difficult hybrids, narrow conditions for material propagation, and inconvenient control of conditions, etc. Achieve the effects of low seed production cost, accelerated breeding process, and free combination

Inactive Publication Date: 2012-04-18
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the utilization of heterosis in cotton mainly adopts artificial detasseling pollination method and "one-line dual-use" method of nuclear sterile line, but these two methods are cumbersome and the cost of seed production is too high, so it is difficult to realize the large-scale application of hybrids
[0003] In the invention patent "A Cotton Sterile Line Propagation and Its Supporting Seed Production Method" (Patent No. 94106074.8), the light-temperature-sensitive sterile cotton material with bud yellow mark needs to be 3-5 years before the flowering period of effective flowers. From one day to the end of the effective flowering period, control the soil temperature 5-15cm below the ground surface at 26.9-36°C to make the bolls form, so that the fertility of the material is controlled by the soil temperature below the ground, and the conditions for the reproduction of the sterile line It is very inconvenient to control
In the invention patent "Cotton GMS Line Yahuang A Light-temperature Interaction Propagation Method" (Patent No. 200510071011.X), the GMS line Yahuang A needs to be controlled at 29.5-30.5°C and 12-13 hours of light In the case of small hours, natural self-breeding is carried out, which makes the material breeding conditions narrow, and it is difficult to meet suitable breeding conditions in the field to obtain a large number of self-breeding

Method used

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  • Breeding method of yellow bud marker photosensitive male sterile line
  • Breeding method of yellow bud marker photosensitive male sterile line
  • Breeding method of yellow bud marker photosensitive male sterile line

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1, Acquisition of Bud Yellow Marked Photosensitive Male Sterile Cotton

[0018] A total of 10 materials of spring cotton (Zhong 50191, Zhong 04002, Zhong 040029, Zhong A3023, Zhong A3025) and summer cotton (Zhong 501, Zhong 502181, Zhong YS-4, Zhong 030415, Zhong 030041) seeds were collected in September 2006. On September 9th, the Shijian No. 8 breeding satellite was carried into space, and it operated for a total of 355 hours in a low-earth orbit with a perigee of 187km and an apogee of 463km, with a voyage of more than 9 million kilometers. Go back and get the seed.

[0019] 1. In October 2006, the seeds after aerial breeding and the wild-type control were planted in Sanya, Hainan, and through seedling screening, a plant with a bud yellow marker was found in the space mutation material of Zhong040029 (see figure 1 ) mutant (T0 generation). The cotyledon stage of the mutant showed bud yellow traits, and the chlorophyll content measured by the APAD-502Plus ch...

Embodiment 2

[0028]Embodiment 2, the breeding (selfing) of Yahuang marker photosensitive male sterile line

[0029] 1. Cultivated in Anyang, Henan

[0030] Henan is located between 110°21′~116°39′ east longitude and 31°23′~36°22′ north latitude. Anyang is located between 113°37' to 114°58' east longitude and 35°12' to 36°22' north latitude.

[0031] Planted 85 grains of the photosensitive male sterile line (seeds) labeled with Yahuang in Anyang, Henan Province in April 2010, and germinated 85 seedlings, all of which had the trait of Bud Huang, and continued to cultivate the seedlings until all plants had no pollen grains until the end of August produce (see figure 2 ), showing infertility. From the perspective of cotton growth cycle, for cotton planted in April, the temperature change and light cycle in the environment are related to pollen development since July, so the temperature and light cycle in July and August were recorded. From July 1st to August 31st, the sunshine time in An...

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Abstract

The invention discloses a breeding method of a yellow bud marker photosensitive male sterile line. The breeding method provided by the invention comprises the following step: performing autocopuation on the yellow bud marker photosensitive male sterile line under the condition of being within 11 hours and 12 hours and 20 minutes so as to get seeds of the yellow bud marker photosensitive male sterile line. The yellow bud marker photosensitive male sterile line is the sterile line, is also a maintainer line of the sterile line, and further has the characteristics of a wide range of restoration sources, free assembly, simple seed production procedure and low seed production cost. The yellow bud marker photosensitive male sterile line comprises a yellow bud marker, hybrid seeds can be effectively removed by screening markers during the seedling stage and photosensitive sterile strains can be further determined. The yellow bud marker photosensitive male sterile line can provide seed resources for breeding of the new photosensitive sterile lines, greatly reduce the cost of artificial emasculation, assemble a large number of hybrid combinations, breed the hybrid seeds and accelerate the breeding process.

Description

technical field [0001] The invention relates to a breeding method of a yellow-marked photosensitive male sterile line. Background technique [0002] Cotton has very obvious heterosis. Although cotton CMS has achieved "three-line matching", due to its narrow recovery source and the negative effect of sterile cytoplasm on the yield of hybrid F1 generation lint, the "three-line" hybrid cotton Breeding progress is relatively slow. At present, the utilization of heterosis in cotton mainly adopts artificial detasseling pollination method and "one-line dual-use" method of GMS line, but these two methods are cumbersome and the cost of seed production is too high, so it is difficult to realize the large-scale application of hybrids. [0003] In the invention patent "A Cotton Sterile Line Propagation and Its Supporting Seed Production Method" (Patent No. 94106074.8), the light-temperature-sensitive sterile cotton material with bud yellow mark needs to be 3-5 years before the flowerin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/04
Inventor 喻树迅范术丽宋美珍马建辉庞朝友魏恒玲
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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