Method for breeding male sterile line by use of sterile cytoplasm of hibiscus cannabinus KN250

A technology of male sterile lines and male sterility, which is applied in botany equipment and methods, application, plant gene improvement, etc., can solve the problem of single cytoplasmic source of kenaf hybrids, and reduce the risk of large-scale occurrence of certain diseases , The effect of low cost of seed production

Active Publication Date: 2014-04-23
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the problem of a single cytoplasmic source of kenaf hybrids, and to provide a method for breeding a new type of kenaf cytoplasmic male sterile line, that is, a method for breeding male sterile lines using the sterile cytoplasm of kenaf KN250

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1 is transformed sterile line with kenaf wild species KN250

[0025] Using the wild species of kenaf KN250 as the female parent and Yuefeng No. 1 B (see: National Invention Patent ZL200510019454.4 Example 3) as the male parent, using the method of saturated backcrossing, a KN250 male sterile cytoplasmic The sterile line K5P3A and its maintainer line K5P3B.

[0026] details as follows:

[0027] From 2008 to 2010, KN250 was used as the female parent and Yuefeng 1B was used as the male parent, and then Yuefeng 1B was used as the male parent to backcross. At the same time of backcrossing, it was crossed with Fuhong 992 and R1 as the male parent. The male parent is crossed, and the combining ability is determined. For each generation, male sterile plants with high combining ability, similar characteristics to the male parent, and no anther dehiscence are selected to continue backcrossing, and the male parent is self-crossed at the same time. Backcrossing is carr...

Embodiment 2

[0028] Embodiment 2 with KN250 seed propagating progeny gained sterile plant transformation sterile line

[0029] Naturally pollinated seeds of kenaf wild species KN250 are harvested, the seeds are planted to reproduce offspring, and sterile plants are obtained by screening. With the obtained sterile strain as the female parent and Yuefeng No. 1 B (see: National Invention Patent ZL200510019454.4 Example 3) as the male parent, the saturated backcross method was used to select a male sterile strain with KN250 cytoplasm CMS line K5TP3A and its maintainer line K5TP3B.

[0030] details as follows:

[0031] From 2010 to 2012, the male sterile plant found in the offspring of KN250 seeds was used as the female parent, and Yuefeng No. 1 B was used as the male parent to cross, and then Yuefeng No. 1 B was used as the male parent to backcross. At the same time, cross with Fuhong 992 and R1 as the male parent, and determine the combining ability. For each generation, male sterile plants...

Embodiment 3

[0032] Example 3 Transformation of sterile lines with kenaf wild species KN250

[0033] Using the wild kenaf species KN250 as the female parent and the conventional kenaf cultivar Jinguang Wuthorn (introduced by Fujian Agriculture and Forestry University) as the male parent, a sterile line with the cytoplasm of the KN250 male sterile line was bred by saturated backcrossing K5K3A and its maintainer line K5K3B.

[0034] details as follows:

[0035] From 2010 to 2012, KN250 was used as the female parent, and Jinguangwuji was used as the male parent, and then Jinguangwuji was used as the male parent for backcrossing. At the same time as the backcrossing, Fuhong 992 and R1 were used as the male parent to determine the Combining ability, male sterile plants with high combining ability, similar traits to the male parent, and no anther dehiscence are selected for each generation to continue backcrossing, and the male parent is self-crossed at the same time. Backcrossing is carried ou...

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PUM

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Abstract

The invention discloses a method for breeding a male sterile line by use of the sterile cytoplasm of hibiscus cannabinus KN250, which specifically comprises the following steps: performing saturation backcross by taking a breeding material containing the sterile cytoplasm of hibiscus cannabinus KN250 as a female parent and the cultivated variety of hibiscus cannabinus as a male parent, and selecting a uniform wild abortive type cytoplasmic male sterile line similar to the male parent in character and showing male sterility, wherein the male parent for backcross is a corresponding male sterility maintainer line. The method disclosed by the invention has the beneficial effects that the hybrid matched with the KN250 cytoplasmic male sterile line and the hybrid matched with the UG93 or H040 cytoplasmic male sterile line are popularized and utilized at the same time, and the occurrence risk of certain disease can be greatly reduced.

Description

technical field [0001] The invention relates to a crop breeding method, in particular to a method for breeding male sterile lines by utilizing the sterile cytoplasm of kenaf KN250. Background technique [0002] Kenaf, also known as "kenaf", is an annual bast fiber crop of the Malvaceae Hibiscus genus. Because kenaf is cultivated for the purpose of harvesting bast fibers or stalks, the heterosis rate is as high as 35% to 40%. The utilization of kenaf heterosis has long been the main goal of kenaf breeding at home and abroad. After years of research, the Cannabis Research Institute of the Chinese Academy of Agricultural Sciences invented the chemical male-killing hybrid seed production method of kenaf and applied it in kenaf breeding. However, because kenaf is an infinite inflorescence, multiple application of pesticides is required, and insect pollination is affected after application, so artificial pollination is required. In order to save costs, the F2 generation (the sec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 赵艳红廖小芳周瑞阳陈鹏周琼周步进
Owner GUANGXI UNIV
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