A herbicide-resistance-based rape hybrid seed producing method and applications thereof

Abstract

The invention discloses a herbicide-resistance-based rape hybrid seed producing method and applications thereof, and relates to the field of plant seed production. The method includes (1) introducing a wide-spectrum type herbicide-resistant gene into a rape male parent line to obtain a herbicide-resistant rape male parent line that is an imidazolidinone-resistant line 12WH381 or a glufosinate-resistant line Z7B10, (2) pollinating a female parent that is sterile after a chemical androcidal action by utilizing pollen of the herbicide-resistant rape male parent line, and harvesting hybrid seeds from male parent plants, and (3) coating the hybrid seeds with a herbicide, or spraying a corresponding herbicide to the hybrid seeds when the hybrid seeds are planted to remove false hybrid seeds at the same time of removing weeds so as to obtain high-purity rape hybrid seeds or group. The applications include (1) hybrid seed production for rape, (2) improvement on rape three-line seed production methods at present, (3) improvement on rape nucleus-sterility two-line seed production methods at present and (4) improvement on rape chemical androcidal seed production systems at present.

Description

technical field [0001] The invention relates to the field of plant breeding, in particular to a herbicide resistance-based rapeseed hybrid seed production method and application thereof. Background technique [0002] 1. Overview of hybrid seed production methods of rapeseed [0003] Rapeseed has strong heterosis. Rapeseed Hybrid F 1 Generations can usually increase yield by 20-30% compared to their parents [1][2] . At present, there are mainly three-line method and two-line method for preparing rapeseed hybrids. The three-line method can be divided into the three-line method of cytoplasmic sterile line (sterile line, maintainer line and restorer line) and the three-line method of nuclear sterile line (nuclear sterile line, temporary insurance line and restorer line) [1][2] . The two-line method can be divided into the two-line method of nuclear sterile line, the two-line method of environmentally sensitive nuclear sterile line, the two-line method of chemical andricide...

AI Technical Summary

Problems solved by technology

The main disadvantages of the cytoplasmic male sterile three-line method are: 1) the cultivation and reproduction procedures of the three-line parents are complicated, the isolation conditions are strict, the three-line breeding cycle is long, and the breeding and seed production costs are high; 2) the cytoplasmic male sterile Material requirements are high, and most cytoplasmic male sterile lines have poor stability and can produce a small amount of pollen, resulting in low purity of hybrids; 3) The male sterile line can only be restored to fertility by the restorer line containing the corresponding restorer gene. Therefore, the parent The original source is narrow, and many excellent germplasms cannot be used directly, which reduces the genetic diversity of the popularized varieties; 4) The sterile cytoplasm has a negative effect on the yield, which limits the production potential of hybrids

[0009] Self-incompatibility two-line seed production method has 4 major defects [3] : 1) The breeding and multiplication of self-incompatible lines is troublesome, and a large amount of artificial pollination is required. Even if the salt water method is used repeatedly, the seed setting rate is very low, and it is difficult to provide high-purity parents for large-scale seed production; 2 ) Continuous selfing may lead to decline in viability; 3) Lack of morphological indicators, making it difficult to identify and remove impurities; 4) Sensitive to environmental factors such as high temperature, there are risks in seed production, so it is difficult to produce in the case of increasingly fierce competition in the seed industry. big development

[0010] It can be seen from the above that the current hybrid rapeseed production methods mainly have the following problems: 1) the parental breeding program is complicated, the cycle is long, and the cost is high; 2) the genetic diversity of the parents is low, which restricts the heterosis potential of rapeseed; 3) the hybrid purity is low ; 4) It is inconvenient to implement chemical weeding

In addition, weeds can act as vectors for certain pests and diseases, and toxic weeds and grass seeds can greatly reduce crop quality

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