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Method for breeding male sterile line by use of sterile cytoplasm of hibiscus cannabinus KN142

A KN142, male sterile line technology, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problem of single cytoplasm source of kenaf hybrids, and reduce the risk of a certain disease. low cost effect

Active Publication Date: 2014-04-23
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the problem of a single cytoplasmic source of kenaf hybrids, and to provide a method for breeding a new type of kenaf cytoplasmic male sterile line, that is, a method for breeding male sterile lines using the sterile cytoplasm of kenaf KN142

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Transformation of sterile lines with kenaf wild species KN142

[0025] Using the wild species of kenaf KN142 as the female parent and Yuefeng No. 1 B (see: National Invention Patent ZL200510019454.4 Example 3) as the male parent, the KN142 male sterile cytoplasm was bred by the saturated backcross method. The sterile line K4P3A and its maintainer line K4P3B.

[0026] details as follows:

[0027] From 2008 to 2010, KN142 was used as the female parent, and Yuefeng 1B was used as the male parent, and then Yuefeng 1B was used as the male parent, and Fuhong 992 and R1 were used as the father during backcrossing. In this cross, the combining ability is determined, and male sterile plants with high combining ability, similar traits to the male parent, and no anther dehiscence are selected for each generation to continue backcrossing, and the male parent is self-crossed at the same time. Backcrossing is carried out in Nanning, Guangxi and Lingshui, Hainan every year...

Embodiment 2

[0028] Embodiment 2 uses KN142 seed propagation progeny gained sterile plant to transform sterile line

[0029] Naturally pollinated seeds of kenaf wild species KN142 were harvested, the seeds were planted to reproduce offspring, and sterile plants were obtained by screening. Using the obtained sterile strain as the female parent and Yuefeng No. 1 B (see: National Invention Patent ZL200510019454.4 Example 3) as the male parent, a KN142 male sterile cytoplasmic strain was bred by means of saturated backcrossing. CMS line K4TP3A and its maintainer line K4TP3B.

[0030] details as follows:

[0031] From 2010 to 2012, the male sterile plant found in the offspring of KN142 seeds was used as the female parent, and Yuefeng No. 1 B was used as the male parent to cross, and then Yuefeng No. 1 B was used as the male parent to backcross. At the same time, Fuhong 992 and R1 were used as the male parent to cross, and the combining ability was determined. For each generation, the male ste...

Embodiment 3

[0032] Example 3 Transformation of sterile lines with kenaf wild species KN142

[0033] Using the wild kenaf species KN142 as the female parent and the conventional kenaf cultivar Jinguang Wuthorn (introduced by Fujian Agriculture and Forestry University) as the male parent, a sterile line with the cytoplasm of the KN142 male sterile line was bred by saturated backcrossing K4K3A and its maintainer K4K3B.

[0034] details as follows:

[0035] From 2010 to 2012, KN142 was used as the female parent, and Jinguang Wuji was used as the male parent, and then Jinguangwuji was used as the male parent to backcross. At the same time as the backcross, Fuhong 992 and R1 were used as the male parent to determine Combining ability, male sterile plants with high combining ability, similar traits to the male parent, and no anther dehiscence were selected for each generation to continue backcrossing, and the male parent was self-crossing at the same time. Backcrossing is carried out in Nannin...

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Abstract

The invention discloses a method for breeding a male sterile line by use of the sterile cytoplasm of hibiscus cannabinus KN142, which specifically comprises the following steps: performing saturation backcross by taking a breeding material containing the sterile cytoplasm of hibiscus cannabinus KN142 as a female parent and the cultivated variety of hibiscus cannabinus as a male parent, and selecting a uniform wild abortive type cytoplasmic male sterile line similar to the male parent in character and showing male sterility, wherein the male parent for backcross is a corresponding male sterility maintainer line. The method disclosed by the invention has the beneficial effects that the hybrid matched with the KN142 cytoplasmic male sterile line and the hybrid matched with the UG93 or H040 cytoplasmic male sterile line are popularized and utilized at the same time, and the occurrence risk of certain disease can be greatly reduced.

Description

technical field [0001] The invention relates to a crop breeding method, in particular to a method for selecting a male sterile line by utilizing the sterile cytoplasm of kenaf KN142. Background technique [0002] Kenaf, also known as "kenaf", is an annual bast fiber crop of the Malvaceae Hibiscus genus. Because kenaf is cultivated for the purpose of harvesting bast fibers or stalks, the heterosis rate is as high as 35% to 40%. The utilization of kenaf heterosis has long been the main goal of kenaf breeding at home and abroad. After years of research, the Cannabis Research Institute of the Chinese Academy of Agricultural Sciences invented the chemical male-killing hybrid seed production method of kenaf and applied it in kenaf breeding. However, because kenaf is an infinite inflorescence, multiple application of pesticides is required, and insect pollination is affected after application, so artificial pollination is required. In order to save costs, only to promote F in pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 廖小芳赵艳红周瑞阳陈鹏周琼周步进
Owner GUANGXI UNIV
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