Breeding method for chilli pepper nuclear male sterile dual purpose line and nuclear substance male sterile recovery line

A dual-purpose line and restorer line technology, applied in botany equipment and methods, application, plant genetic improvement, etc., can solve the problems of long breeding time and few resources of good restorer lines, so as to increase the purity and reduce the cost of seed production Effect

Active Publication Date: 2006-08-23
北京市海淀区植物组织培养技术实验室
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Problems solved by technology

[0010] Aiming at the problem of long breeding time in the method of breeding dual-purpose lines by conventional backcrossing, and in order to solve the problem of few excellent restorer line resources in the current research on male sterility of peppers, the present invention discloses dual-purpose lines using peppers and Method for Screening Sterile Dual-purpose Lines and Nucleoplasmic Interaction Male Sterile Restorer Lines by Three-line Hybrids

Method used

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  • Breeding method for chilli pepper nuclear male sterile dual purpose line and nuclear substance male sterile recovery line
  • Breeding method for chilli pepper nuclear male sterile dual purpose line and nuclear substance male sterile recovery line

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Embodiment 1

[0026] Embodiment 1: the selection and breeding of capsicum male sterile dual-purpose lines

[0027] 1. Breeding process of male sterile dual-purpose lines of pepper

[0028] In 1998, a dual-purpose material of croissant pepper was introduced from the Northeast. Its fruit is small croissant, short, dark green, wrinkled, and the system is not neat. It is numbered 98-807. Breeding work for nuclear male sterility source.

[0029] QB-18 is an inbred line for many years. The fruit has thick horns, yellow-green fruit color, and smooth fruit surface. It is the backbone parent material of sheep horn pepper hybrids. This was used as the male parent of the male sterile dual-purpose line.

[0030] In 98-807, a sterile single plant was selected to cross with QB-18, that is, 98-807-9×QB-18. refer to figure 1 The breeding map of the male sterile dual-purpose line of pepper. The seeds of F1 were planted in 1999, and flower buds were taken for inoculation at the seedling stage. A total of...

Embodiment 2

[0047] Example 2: Screening of Capsicum Nucleoplasmic Male Sterile Restorer Lines

[0048] In 2000, in the offspring of Biyu and Nongda 23, some fertile plants were selected for test crossing with the contemporary sterile lines, and the fertile plants were used for test cross pollination to the existing sterile lines. Continuously observe the fertility of F1 in the winter greenhouse and in the open field in spring, and select F1 with a fertility recovery rate of 100% and stable fertility, and the corresponding fertile plants are restorer plants, and their self-bred offspring lines are restorer lines .

[0049] 1. Screening process of pepper cytoplasmic male sterile restorer lines

[0050] 1.1 Anther culture of three-line hybrids

[0051] In 1999, the anthers of the three-line hybrids "Biyu" and "Nongda 23" were inoculated, and the number of inoculated anthers were 589 and 1478 respectively, and 216 and 349 embryoids were obtained, and the frequency of embryoid induction was ...

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Abstract

The present invention relates to plant breeding technology, and is especially anther cultivation technological method of breeding unclear sterile dual purpose line and unclear substance sterile recovery line of capsicum. Extracorporeal anther cultivation technology is used in selective breeding of unclear sterile dual purpose line and unclear substance sterile recovery line of capsicum. That is, the dual purpose line is obtained through test cross of the fertile plant and sterile plant in contemporary anther cultivation offspring of dual purpose line hybrid, F1 selfing, test cross of similar fertile plant and sterile plant in the offspring lines and continuous two generation selection; and the recovery line is obtained through the test cross of the fertile plant and sterile plant in the contemporary anther cultivation offspring of three line hybrid with available sterile line, selectively breeding the F1 with fertility recovering rate of 100 % and stable fertility to obtain fertile plant as the corresponding recovering plant and final selfing.

Description

technical field [0001] The invention belongs to the technical field of breeding methods for plant hybrids, and in particular relates to a breeding method for selecting male sterile lines of capsicum and screening male sterile restorer lines using anther culture technology. Background technique [0002] At present, the capsicum hybrid breeding technology widely used at home and abroad is a conventional breeding technology, which generally includes the following steps: first, several capsicum inbred lines (lines) are selected, and then two inbred lines are artificially emasculated and paired for pollination. A hybrid combination is prepared, and then through a series of selective breeding processes, hybrids that can be popularized and applied in production are bred; the conventional technical method of using male sterility is to find male sterile plants in natural populations of peppers, and then use them Testcross a large number of fertile plants in the population, screen the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
Inventor 邢永萍张树根蒋钟仁李春玲
Owner 北京市海淀区植物组织培养技术实验室
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