Breeding method for chilli pepper nuclear male sterile dual purpose line and nuclear substance male sterile recovery line
A dual-purpose line and restorer line technology, applied in botany equipment and methods, application, plant genetic improvement, etc., can solve the problems of long breeding time and few resources of good restorer lines, so as to increase the purity and reduce the cost of seed production Effect
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Embodiment 1
[0026] Embodiment 1: the selection and breeding of capsicum male sterile dual-purpose lines
[0027] 1. Breeding process of male sterile dual-purpose lines of pepper
[0028] In 1998, a dual-purpose material of croissant pepper was introduced from the Northeast. Its fruit is small croissant, short, dark green, wrinkled, and the system is not neat. It is numbered 98-807. Breeding work for nuclear male sterility source.
[0029] QB-18 is an inbred line for many years. The fruit has thick horns, yellow-green fruit color, and smooth fruit surface. It is the backbone parent material of sheep horn pepper hybrids. This was used as the male parent of the male sterile dual-purpose line.
[0030] In 98-807, a sterile single plant was selected to cross with QB-18, that is, 98-807-9×QB-18. refer to figure 1 The breeding map of the male sterile dual-purpose line of pepper. The seeds of F1 were planted in 1999, and flower buds were taken for inoculation at the seedling stage. A total of...
Embodiment 2
[0047] Example 2: Screening of Capsicum Nucleoplasmic Male Sterile Restorer Lines
[0048] In 2000, in the offspring of Biyu and Nongda 23, some fertile plants were selected for test crossing with the contemporary sterile lines, and the fertile plants were used for test cross pollination to the existing sterile lines. Continuously observe the fertility of F1 in the winter greenhouse and in the open field in spring, and select F1 with a fertility recovery rate of 100% and stable fertility, and the corresponding fertile plants are restorer plants, and their self-bred offspring lines are restorer lines .
[0049] 1. Screening process of pepper cytoplasmic male sterile restorer lines
[0050] 1.1 Anther culture of three-line hybrids
[0051] In 1999, the anthers of the three-line hybrids "Biyu" and "Nongda 23" were inoculated, and the number of inoculated anthers were 589 and 1478 respectively, and 216 and 349 embryoids were obtained, and the frequency of embryoid induction was ...
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