Breeding and seed producing method for recessive gene male sterile line of cauliflower

Abstract

The invention relates to a breeding and seed producing method for a recessive gene male sterile line of cauliflower. The invention adopts the technical scheme that a recessive gene male sterile mutant strain of the cauliflower is subjected to sister hybrid and progeny selfing separation to breed a sterile strain with the sterile rate of 100 percent and the sterile degree reaching or approximating to 100 percent; the sterile line used as a female parent is hybridized and combined with an excellent selfing line; and a combined hybrid seed is produced by applying a honeybee pollination method. The invention has the advantages that: firstly, a new sterile source for heterosis advantage utilization of the cauliflower is provided and the defects of cytoplasmic sterility are overcome; secondly, the recessive gene male sterile line is maintained and reproduced by using a tissue culture technology and various works for filed isolation and preproduction of the sterile line and a maintenance line are omitted, so that the influence on preproduction of the sterile line caused by weather, season, land and isolating conditions is avoided; and thirdly, a hybrid strain is produced by using the recessive gene male sterile line and honey can be used for pollination, and thus the seed production cost is reduced and the seed production efficiency is improved.

Description

technical field [0001] The invention relates to a method for utilizing vegetable heterosis, in particular to a method for breeding and seed production of recessive nuclear male sterile lines of cauliflower. Background technique [0002] Cauliflower is a Brassicaceae Brassica plant. It is an important vegetable crop in my country. It has obvious heterosis. The first generation of heterosis has been widely used in production. There are three main ways to produce first generation hybrid seeds of cauliflower. One is to use artificial bud-stripping hybridization, the other is to use self-incompatibility, and the third is to use cytoplasmic male sterility. The first two methods have been widely used in production, but the first method is labor-intensive and time-consuming, the cost of seed production is high, and the hybridization rate cannot reach 100%, and the quality of the seeds is unstable; self-incompatible lines are difficult to reproduce. Parental multigenerational self-...

AI Technical Summary

Problems solved by technology

However, most of the research on male sterility of cauliflower in my country is to transfer the Ogura radish cytoplasmic sterile source introduced from abroad into cauliflower to produce a cytoplasmic sterile line, but the sterile source has small nectary glands and cannot attract insects normally. Disadvantages such as pollination and abnormal flower organs limit its large-scale application in production

At present, there are still relatively few cauliflower male sterile lines available in my country, and the main source is the Ogura sterile source, which has a single cytoplasm and close blood relationship, which may lead to a narrow genetic basis for the configuration of hybrids, resulting in reduced resistance to the environment

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