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Method for quickly breeding dendrobium officinale

A fast technology of Dendrobium candidum, applied in the field of rapid breeding of Dendrobium candidum and large-scale production of high-quality Dendrobium candidum test-tube seedlings, can solve the problems of being unsuitable for Dendrobium candidum strains, wasting seedlings of Dendrobium candidum, unable to continue to grow, etc., achieving easy operation and adaptability The effect of good sex and fast reproduction

Active Publication Date: 2015-05-20
杭州九圣生物科技有限公司
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AI Technical Summary

Problems solved by technology

For example, currently there are quite a lot of technologies that directly use Dendrobium officinale or its seedlings as initial culture materials, such as Chinese patents 200610017604, 200910051663, 201310282962, 200910238037, 201010202793, 201210374262, 201310186625, 2013102829, etc. It is actually a waste of 5 The high-quality Dendrobium officinale seedlings, especially high-quality Dendrobium officinale seedlings, if the efficiency of tissue culture is not high, then it is meaningless, and the high-quality Dendrobium officinale seedlings may even be wasted due to tissue culture failure;
[0005] 另外许多技术是使用铁皮石斛(苗)的(幼)茎、茎尖或芽,如中国专利200710160571、200810058043、200810058044、200810058316、200910164340、201010000764、201110035697、201110284479、201110365328、201310146960等,这样破坏了茎, It makes plants with erect stems like Dendrobium candidum unable to continue to grow, and there will also be problems caused by the low efficiency of the above tissue culture, especially not suitable for high-quality Dendrobium candidum strains

Method used

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  • Method for quickly breeding dendrobium officinale
  • Method for quickly breeding dendrobium officinale
  • Method for quickly breeding dendrobium officinale

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The method 1 of embodiment 1 rapidly breeding Dendrobium officinale

[0044] Prepare medium as follows:

[0045] 1) Basic medium: agar-containing formula: MS basic medium + sucrose 25g / L + agar 7g / L, pH 5.6; formula without agar: MS basic medium + sucrose 25g / L, pH 5.6;

[0046] 2) Embryogenic callus induction medium: agar-containing basic medium + hydrolyzed milk protein 1g / L + ABA (abscisic acid) 0.7mg / L + BA (6-benzylaminopurine) 1mg / L + NAA (naphthaleneacetic acid ) 1mg / L;

[0047] 3) Protocorm liquid suspension medium: 1 / 2 basic medium without agar + ABA0.3mg / L+BA0.3mg / L+NAA0.1mg / L;

[0048] 4) Subculture growth medium: basic medium containing agar + BA0.1mg / L + NAA0.1mg / L + potato (powder) 0.3kg / L;

[0049] 5) Rooting medium: basic medium containing agar + NAA0.1mg / L;

[0050] The tissue culture steps of Dendrobium officinale are as follows:

[0051] 1) Material pretreatment: Take fresh non-bud leaves and rinse them with tap water for 10-30 minutes, then imme...

Embodiment 2

[0056] The method 2 of embodiment 2 rapidly breeding Dendrobium officinale

[0057] Prepare medium as follows:

[0058] 1) Basic medium: agar-containing formula: MS basic medium + sucrose 25g / L + agar 8g / L, pH 5.7; formula without agar: MS basic medium + sucrose 25g / L, pH 5.7;

[0059] 2) Embryogenic callus induction medium: basic medium containing agar + hydrolyzed milk protein 1g / L + ABA0.9mg / L + BA1.3mg / L + NAA0.7mg / L;

[0060] 3) Protocorm liquid suspension medium: 1 / 2 basic medium without agar + ABA0.3mg / L+BA0.5mg / L+NAA0.2mg / L;

[0061] 4) Subculture growth medium: basic medium containing agar + BA0.2mg / L + NAA0.2mg / L + potato (powder) 0.3kg / L;

[0062] 5) Rooting medium: basic medium containing agar + NAA0.2mg / L;

[0063] The tissue culture step of Dendrobium officinale is the same as embodiment 1, and the test-tube plantlet that finally produces is as follows: figure 1 As shown, it can be used for field transplantation, and every fresh non-bud leaf is picked (withou...

Embodiment 3

[0064] The method 3 of embodiment 3 rapidly breeding Dendrobium officinale

[0065] Prepare medium as follows:

[0066] 1) Basic medium: agar-containing formula: MS basic medium + sucrose 25g / L + agar 9g / L, pH 5.8; formula without agar: MS basic medium + sucrose 25g / L, pH 5.8;

[0067] 2) Embryogenic callus induction medium: basic medium containing agar + hydrolyzed milk protein 1g / L + ABA1.1mg / L + BA1.5mg / L + NAA0.9mg / L;

[0068] 3) Protocorm liquid suspension medium: 1 / 2 basic medium without agar + ABA0.5mg / L+BA0.7mg / L+NAA0.3mg / L;

[0069] 4) Subculture growth medium: basic medium containing agar + BA0.3mg / L + NAA0.3mg / L + potato (powder) 0.3kg / L;

[0070] 5) Rooting medium: basic medium containing agar + NAA0.3mg / L;

[0071] The tissue culture procedure of Dendrobium officinale is the same as in Example 1.

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Abstract

The invention belongs to the field of plant breeding or tissue culture, and particularly relates to a method for quickly breeding dendrobium officinale. The method comprises the following steps: cutting a blade on a dendrobium officinale plant into leaf blocks; cultivating the leaf blocks into embryonic callus on an embryonic callus induction medium; cultivating the embryonic callus into a protocorm on a protocorm liquid suspension culture medium; cultivating the protocorm into plantlets on a transgenerational growth medium; cultivating the plantlets into test-tube plantlets on a root medium.

Description

technical field [0001] The invention belongs to the field of plant breeding or tissue culture. Specifically, the invention relates to a method for rapid breeding of Dendrobium officinale, and is especially suitable for large-scale production of high-quality Dendrobium officinale test-tube seedlings. Background technique [0002] Dendrobium officinale (scientific name: Dendrobium officinale), also known as black knot grass, belongs to the aerial orchid family herb. Dendrobium can be divided into dozens of species such as yellow grass, nobile, horsewhip, etc. Among them, Dendrobium candidum is the best of Dendrobium, and it gets its name because of its iron-green skin. It is native to Dabie Mountain in Anhui, Tiantai Mountain in eastern Zhejiang, Ninghua in Fujian, Tian'e in Guangxi, Wenshan in Yunnan, and Sichuan in China. It is mostly distributed on semi-shady and humid rocks in mountainous areas with an altitude of nearly 1,000 meters. Generally, it can withstand a low temp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 严成其俞洁张玲菊林明斯金平朱玉球沈岚刘健王芳朱宏芬张国芳龚松年黄坚
Owner 杭州九圣生物科技有限公司
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