A treatment method for inducing corn parthenogenesis with colchicine

A technology of colchicine and parthenogenety, which is applied in the field of corn breeding, can solve the problems of high pollen pollution rate and many false seeds, and achieve the effect of simple operation, simple procedure and simple procedure

Active Publication Date: 2017-01-04
SHENYANG TEYIJIA CORN TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is necessary to take advantage of the advantages of the high induction rate of colchicine, and to solve the safety problems in the existing methods in the operation method; (2) to solve the common problems of high pollen pollution rate and many false seeds in the existing methods of chemical induction ; (3) Simplify the processing procedure, make the operation simple and easy for large-scale application; (4) Simultaneously improve the induction rate and reduce the dosage of the drug

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] (1) Preparation of inducer

[0049] Colchicine (COL) was diluted with water to a concentration of 0.1% inducer.

[0050] (2) Select processing test material

[0051] The object of treatment is the maize hybrid "Liaodan 565".

[0052] (3) Ear treatment before induction

[0053] Ears are bagged before silking. Take off the paper bag after the filaments are fully spit out, cut off the upper flower buds horizontally at the position where the ear buds are close to the young ears of the ear from the top, and cut off a section of filaments on the section with an arc-shaped carving knife. The depth of cutting is 5- 15mm, do not cut out the surrounding bracts, forming a cavity with a depth of 5-15mm.

[0054] (4) Apply inducer

[0055] Inject the inducer into the cavity with a pipette to fill and cover or wet the entire cut surface. Finally put back into the paper bag. 60 spikes were processed.

[0056] Operating conditions: The operating process is carried out under com...

Embodiment 2

[0062] (1) Preparation of inducer

[0063] Colchicine and dimethyl sulfoxide (DMSO) sold in the market are selected, and the inducer is compounded according to the following weight ratio: 0.2% colchicine, 2% dimethyl sulfoxide, and the balance is water.

[0064] (2) Select processing test material

[0065] The object of treatment is sweet corn hybrid "Shentian 6".

[0066] (3) Ear treatment before induction

[0067] Ears are strictly bagged before silking. When the filaments are fully spit out, take off the paper bag, cut off the upper flower bud horizontally at the position where the ear bud is close to the young ear of the ear from the top, and then cut off a section of filament on the section with an arc-shaped carving knife. The cutting depth is 5-5. 15mm, do not cut out the surrounding bracts, forming a cavity with a depth of 5-15mm.

[0068] (4) Apply inducer

[0069] Inject the inducer into the cavity with a pipette to fill and cover or wet the entire cut surface. ...

Embodiment 3

[0076] (1) Preparation of inducer

[0077] Select colchicine, dimethyl sulfoxide and green fresh element (MH) sold on the market for use, carry out compound inducer according to the following weight ratio: colchicine 0.2%, dimethyl sulfoxide 2%, 40mg·kg -1 cyanin, the balance is water.

[0078] (2) Select processing test material

[0079] The object of treatment is the maize hybrid "Xianyu 335".

[0080] (3) Ear treatment before induction

[0081] Strictly bag the ears before spinning. When the filaments are completely spit out, take off the paper bag, and cut off the upper flower bud horizontally at the position where the ear bud is close to the ear young ear from the top to form a circular cross section, and then dig out a section of the filament on the section with an arc-shaped carving knife. The depth of cutting is 5-15 mm, and the surrounding bracts are not cut to form a cavity with a depth of 5-15 mm.

[0082] (4) Apply inducer

[0083] Inject the inducer into the...

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PUM

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Abstract

A treatment method for inducing maize parthenogenesis with colchicine. Its characteristic is that when the ear filaments are completely spit out, the paper bag is taken off. Cut off the upper part of the flower bud horizontally from the top down to the young ear of the ear, and then cut off a section of filament on the section with an arc-shaped carving knife to form a cavity with a depth of 5-15mm; put the inducer Fill the cavity with colchicine treatment solution via a pipette or syringe, filling and wetting the entire cut surface. Finally put it back into the paper bag; the treatment process does not require anti-pollen pollution measures such as sealing, shielding, space and time isolation. The positive effect of the present invention is to avoid the threat to human health caused by dripping, overflowing and scratching of the medicinal liquid; completely solve the problem of pollen pollution which is difficult to solve; ensure that all filaments can be in contact with the medicinal liquid and the medicinal liquid stays for a long time , to increase the induction rate; save about 50% of the liquid medicine; and shorten the breeding cycle by more than 5 generations compared with the conventional method of breeding inbred lines.

Description

technical field [0001] The invention belongs to the technical field of corn breeding, in particular to a method for breeding corn inbred lines. Background technique [0002] The conventional method of breeding maize inbred lines requires at least 7 generations of continuous selfing to breed an inbred line, and even more than 10 generations for materials with complex genetic backgrounds. In order to speed up the breeding speed of inbred lines, shorten the breeding cycle and improve the purity, the methods commonly used at home and abroad mainly include: [0003] 1. Colchicine induction method [0004] At the silking stage of maize, colchicine was used as a chromosome doubling agent to treat the filaments so that they could directly produce homozygous diploid seeds through parthenogenesis without double fertilization. [0005] Colchicine is a commonly used chromosome doubling treatment agent, and its outstanding advantage is its high induction rate, so it is widely used in t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/08
Inventor 史振声张喜华
Owner SHENYANG TEYIJIA CORN TECH
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