The invention discloses a method for efficiently purifying
gardenia blue
pigment based on combination of
ion exchange resin and an
ultrafiltration membrane, and belongs to the technical field of natural
pigment purification. The method comprises the following steps: adjusting the pH value of a
gardenia blue
pigment crude product solution to 5.0-7.0, pretreating, loading to anion exchange resin for adsorption, sequentially carrying out
gradient elution by using 5% and 10% NaCl aqueous solutions, collecting pigment eluents with different molecular weight proportions, separating by using an
ultrafiltration membrane with the molecular weight
cut-off of 1000-4000 Da, and finally concentrating and
drying. According to the method, efficient and specific removal of gardenoside,
genipin and
methanol is synergistically realized through charge
selective adsorption and
gradient elution of
ion exchange resin in combination with precise screening of an
ultrafiltration membrane, meanwhile, the pigment
recovery rate is greater than 95%, the product
impurity residue is low, the process is green, and the method is suitable for industrial production.