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Chemical induction corn parthenogenesis operation method

A technology of parthenogenesis and operation method, which is applied in the field of corn breeding to achieve the effects of fast processing speed, safe use and convenient use

Inactive Publication Date: 2016-09-21
SHENYANG AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0018] The technical problems to be solved by the present invention are: (1) to solve the problem of safe use of chemical induction treatment agents, no matter using highly toxic reagent colchicine or herbicide induction, safe operation can be realized; (2) Simplify the treatment method, Reduce breeding procedures and improve processing efficiency; (3) Completely solve the problem of pollen pollution; (4) Realize large-scale application

Method used

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  • Chemical induction corn parthenogenesis operation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Operation method for inducing maize parthenogenesis

[0049] (1) Preparation of inducer

[0050] Dilute colchicine (COL) with water to a 0.15% concentration of inducer.

[0051] (2) Handling test materials

[0052] The processing object is the corn hybrid ‘Xianyu 335’.

[0053] (3) Ear processing

[0054] The tool is an improved needle-nose pliers (see figure 1 ): Use needle-nose pliers with specifications of about 6"~7". Grind the side of the needle-nose pliers without the cutting edge with a grinding wheel to extend the cutting edge part B of the jaw to make a jaw with a single-sided pinching function. At the same time, slightly flatten the cutting edge on one side of the jaws to form a 1mm~2mm gap A when the jaws are closed, and make the tough mouth dull, which is used to pinch and cut the corn filament.

[0055] Strictly bagging the ears before spinning. On the 4th day after the filaments are spit out, take off the paper bag, grab the filaments with your left hand...

Embodiment 2

[0060] Example 2 Operation method for inducing parthenogenesis of waxy corn

[0061] (1) Preparation of inducer

[0062] Choose colchicine and dimethyl sulfoxide (DMSO) on the market, and dilute with water to form an inducer of 0.2% colchicine + 2% dimethyl sulfoxide.

[0063] (2) Handling test materials

[0064] The treatment object is the waxy corn hybrid'Shennuo 3'.

[0065] (3) Ear processing

[0066] Strictly bagging the ears before spinning. 4 days after the filament is spit out, take off the paper bag, grab the filament with your left hand, and hold the improved needle-nose pliers with your right hand. Place the top of the female ear buds down close to the ears of the female ears. Use needle-nose pliers to pinch the female ear buds. When the filaments in the bracts have been cut off. Then slowly pull out the filament by hand, leaving a hole.

[0067] (4) Injection of inducer

[0068] Use a syringe to inject about 3ml of inducer into the hole, so as not to overflow. Finally put i...

Embodiment 3

[0071] Example 3 Operation method for inducing parthenogenesis of sweet corn

[0072] (1) Preparation of inducer

[0073] Choose the colchicine, dimethyl sulfoxide and penicillin (MH) sold in the market, and dilute with water to make 0.2% colchicine + 2% dimethyl sulfoxide + 40mg·kg-1 penicillin Inducer.

[0074] (2) Handling test materials

[0075] The treatment object is the sweet corn hybrid "Shentian No. 6".

[0076] (3) Ear processing

[0077] Strictly bagging the ears before spinning. When the filaments are spit out 4 to 5 days, take off the paper bag, grab the filaments with your left hand, and hold the improved needle-nose pliers in your right hand. Place the buds of the female ears down about 4cm below the ears of the ears, and pinch the ears with needle-nose pliers Bud, when you feel that the filaments in the bracts have been pinched off. Then slowly pull out the filament to form a hole.

[0078] (4) Injection of inducer

[0079] Use a syringe to inject about 3ml~5ml of induc...

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Abstract

The invention provides a chemical induction corn parthenogenesis operation method. The chemical induction corn parthenogenesis operation method is characterized by comprising strictly bagging female ears before filaments are protruded, dropping off paper bags in 4-5 days after the filaments of the female ears are protruded, pinching the filaments under bract isolation conditions by the aid of improved long-nose pliers, pulling out the filaments by hands and reserving a hole after each filament is pulled out; injecting preferably 2-5 ml of inductive agents into each hole by the aid of a syringe; sleeving the female ears with the paper bags to complete treatment procedures. The chemical induction corn parthenogenesis operation method has the advantages that the problems in the aspect of safe use of poisonous reagents can be solved, the problem of existing pollen pollution can be thoroughly solved, and operation procedures are carried out under completely open conditions; good chemical application effects can be realized, operation can be carried out in all seasons, and the chemical induction corn parthenogenesis operation method is easy to implement, high in treatment speed and suitable for large-scale application.

Description

Technical field [0001] The invention belongs to the technical field of corn breeding, in particular to an operation method for chemically inducing parthenogenesis of corn. Background technique [0002] The conventional method of breeding maize inbred lines requires at least 7 generations of continuous selfing to bred an inbred line, and even more than 10 generations for materials with complex genetic background. In order to speed up the selection and breeding of maize inbred lines, shorten the breeding cycle, and improve the purity of inbred lines, chemical induction methods and genetic induction methods are usually used at home and abroad: [0003] 1. Colchicine induction method [0004] Using the principle that colchicine can prevent cell division and cause chromosome doubling, colchicine inducer is used to induce maize parthenogenesis. The main method is to smear the filaments with a certain concentration of colchicine during the spinning period of corn to make it directly produ...

Claims

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Application Information

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IPC IPC(8): A01H1/08
CPCA01H1/08
Inventor 史振声李凤海钟雪梅朱敏王志斌张喜华
Owner SHENYANG AGRI UNIV
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