Bacillus siamensis, microbial agent containing bacillus siamensis and application thereof

A technology for bacillus and inoculum, applied in the field of inoculum and its application, Bacillus siamese, can solve problems to be studied, and achieve the effects of improving yield and quality, improving soil fertility, and being easy to absorb

Active Publication Date: 2020-04-28
SINOCHEM AGRI LINYI R&D CENT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, microorganisms suitable fo

Method used

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  • Bacillus siamensis, microbial agent containing bacillus siamensis and application thereof
  • Bacillus siamensis, microbial agent containing bacillus siamensis and application thereof
  • Bacillus siamensis, microbial agent containing bacillus siamensis and application thereof

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Embodiment 1

[0032] Isolation and screening of embodiment 1 bacterial strain

[0033] (1) Isolation of cellulase-producing strains: collect rotten straw and livestock manure in the test field of Sinochem Agriculture Linyi R&D Center, weigh 1g into 100ml sterile water, shake at 150rpm, 30°C for 30min, and then carry out gradient diluted to 10 5 , select 3 gradients for coating, and each gradient has 3 parallels. After culturing in a 30°C incubator for 2 days, select strains with different colony forms to streak on LB medium, and observe the growth of colonies regularly. Then, the plate streak method was used to purify the strains, and they were numbered and stored separately.

[0034] (2) Screening of cellulase-producing strains: Prepare LB medium (adding sodium carboxymethyl cellulose) by plate hydrolysis circle method, transplant the strains isolated and preserved in (1) into the center of the plate with a toothpick, and keep at 30°C After incubating at constant temperature for 48 hours, ...

Embodiment 2

[0049] The A-2 strain was identified as follows:

[0050] (1) Microbiological characteristics: Cultured on LB medium at 37°C for 2 days to form colonies with a size of about 4 mm, neat edges, raised, white viscous and translucent. The optimum growth temperature is 37°C, the salt tolerance (NaCl) concentration is 14%, the growth pH is 4.0-9.0, and the optimum is 7.0.

[0051] (2) Molecular biological characteristics: The phylogenetic tree of the strain formed by the 16SrDNA sequence of molecular biology shows that the strain has the closest homology to Bacillus siamensis strain XSG14, so the strain is identified by molecular biological identification and microbiological characteristics analysis It is Bacillus siamensis.

[0052] The 16SrDNA sequence of the strain is as follows, and the phylogenetic tree can be found in figure 1 .

[0053] TCTCGTGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGA...

Embodiment 3

[0059] The preparation of embodiment 3 microbial bacterial agents

[0060] Step 1: Inoculate the Bacillus siamese strain A-2 preserved in the plate into an Erlenmeyer flask containing 250ml of LB culture solution with an inoculation loop, and culture it in a constant temperature incubator at 30°C and 170rpm for 36h.

[0061] Step 2: Inoculate the above-mentioned fermented bacteria liquid into a pilot test fermenter (10L-50L-500L), cultivate at 37°C, spray dry after the fermentation is completed, and subpackage. The number of viable bacteria is 10-15 billion / g. Mix it with calcium carbonate to form a bacterial powder with a viable count of 10 billion / g, which is to prepare the Bacillus siamese A-2 bacterial agent for composting. The formula of pilot test medium is: corn starch 3%, sucrose 1%, soybean meal 4.5%, yeast powder 0.2%, peptone 0.2%, dipotassium hydrogen phosphate, potassium dihydrogen phosphate 0.3%, calcium carbonate, sodium chloride 0.1% , 0.3% defoamer, replenish...

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Abstract

The invention provides a microorganism, a microbial agent containing the microorganism, as well as a preparation method and application thereof. The microorganism is bacillus siamensis which is preserved in China General Microbiological Culture Collection Center, under the preservation number of CGMCC No. 18688, from October 15, 2019 on. The microorganism provided by the invention has relatively good ability to decompose cellulose, and can be effectively applied in straw composting so as to obviously accelerate temperature rise rate in an early stage of composting, raise fermentation temperature, increase straw weight loss rate after the composting, decrease C/N content and achieve high compost maturity; and thus, the microorganism is suitable for large-scale application.

Description

technical field [0001] The present invention relates to the field of biology. Specifically, the present invention relates to Bacillus siamese, bacterial agents and applications thereof. Background technique [0002] With the advancement of farming technology, the output of straw is increasing. However, due to the low utilization rate of straw, most of it is burned, which leads to waste of resources and environmental pollution. As a good raw material for organic fertilizer production, straw has not been fully utilized due to the lack of mature and efficient conversion technology. Returning the straw to the field can make full use of the nutrient resources in the straw and improve the soil. After returning to the field, the straw decomposes under the action of soil microorganisms, and the cellulose, hemicellulose, and lignin in the straw are converted into simple compounds that are easily absorbed by plants, thereby improving the physical and chemical properties of the soil,...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G1/00C05F17/20C05F11/08C12R1/07
CPCC05C9/00C05F11/08C12N1/20C12N1/205C12R2001/07Y02W30/40
Inventor 王子浩王峥张西兴邱鹏飞李洪顺
Owner SINOCHEM AGRI LINYI R&D CENT CO LTD
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