7 results about "Virus receptor" patented technology

The present disclosure provides an immunodeficient or immune-impaired chimeric non-human animal with a deletion or impairment of adeno-associated virus receptor (AAVR), comprising human hepatocytes, methods for preparing the chimeric non-human animal comprising human hepatocytes and methods of utilizing the chimeric non-human animal comprising human hepatocytes to evaluate transduction efficiency of adeno-associated viruses (AAV), and determine mechanism of inhibition / modification of AAV transduction in human hepatocytes.

The application relates to a method for detecting GDNF protein activity in vitro, comprising the following steps: a) contacting an adeno-associated virus (AAV) containing a nucleotide sequence encoding a GDNF protein with first cells expressing an adeno-associated virus receptor (AAVR), culturing the first cells, and collecting culture supernatant; b) incubating the culture supernatant in step a) with second cells, wherein the second cells are reporter gene cells; and c) detecting an indicating signal generated by the reporter gene, wherein the reporter gene cells contain a GFR alpha 1 expression element, a RET expression element, a transcription response module and a reporter gene module; the transcription response module contains a GAL4-ELK1 fusion protein and / or a nucleic acid encoding the fusion protein; and the reporter gene module contains a nucleic acid encoding a GAL4 response element and a reporter gene.

The invention belongs to the technical field of biology, and discloses an influenza B virus sensitizing cell strain as well as a construction method and application thereof. The influenza B virus sensitizing cell strain STX-BB2 can be used for stably expressing influenza virus receptor ST6GAL1 protein in a human source and controllably inducing and expressing influenza B virus PB2 protein by using doxycycline; the preservation number of the cell strain STX-BB2 is GDMCC (China General Microbiological Culture Collection Center) NO: 67100; the cell strain can enhance the expression ability of a host receptor and the virus replication key gene level at the same time; the infection efficiency and the replication titer of the influenza B virus in MDCK cells can be improved; the method can be widely applied to influenza B virus separation, mechanism research, vaccine seed virus amplification and drug screening model construction.

The invention discloses a device suitable for aerosol phase virus-receptor affinity and activity evaluation, an evaluation method and a data analysis method. The device comprises an aerosol generation unit, a bionic artificial cell array unit, an environment control and fixing assembly and an activity evaluation and collection unit. The method is characterized in that through a micro-nano processing and molecular self-assembly technology, a supporting lipid double-layer membrane is constructed on an array substrate, receptor protein is directionally fixed, and a bionic cell interface is formed. When in use, a virus sample is atomized into aerosol and flows through the interface, so that the virus is specifically combined with the receptor; affinity parameters in an aerosol environment can be obtained by combining quantitative detection with viruses and utilizing algorithm model fitting. Meanwhile, the system can directly collect an aerosol sample after action and inoculate cells for culture, so that synchronous verification of virus biological infection activity is realized. The device is easy and convenient to operate and high in universality, and a brand new tool is provided for studying the recognition and infection mechanism of viruses under the real aerosol transmission condition.

The invention relates to the technical field of biological medicines, and discloses a medicine and a method for preventing and / or treating yellow fever virus (YFV) infection. Through whole genome overexpression library screening and experimental verification, it is found that LRP8, VLDLR and LRP4 (especially LRP8) are virus receptors of YFV and are potential antiviral targets. Corresponding inhibitors (such as antibodies and siRNA) and bait proteins (such as soluble proteins of LRP8 ligand binding structural domain LBD fused Fc tags) can effectively neutralize YFV viruses and block virus infection in the cell level and the experimental animal level, and have very good application prospects and drug research and development values in the antiviral field.

The invention relates to a grass carp cell membrane protein gene CRISPR (clustered regularly interspaced short palindromic repeats) activated plasmid library. The library comprises CRISPR activated plasmids for targeted activation of 3498 candidate grass carp cell membrane protein genes; the invention further relates to a recombinant lentivirus capable of infecting fish cells and a packaging system of the recombinant lentivirus. The invention provides a CRISPR (clustered regularly interspaced short palindromic repeats) activation library aiming at related genes of grass carp cell membrane protein for the first time, and further provides recombinant lentivirus capable of infecting fish cells and a packaging system of the recombinant lentivirus. Protein on a grass carp cell membrane is identified through experiments, genes which exist in genome prediction but are not actually expressed can be excluded, and a library which accurately reflects related genes of grass carp cell membrane protein is formed. The CRISPR activation library provided by the invention avoids the situation of cell death after essential genes in a gene knockout library are knocked out, can comprehensively aim at essential or non-essential genes for cell survival, and can be widely applied to scenes such as virus receptor identification and / or gene function research and / or drug screening.

The invention belongs to the technical field of biology, and discloses an influenza virus sensitizing cell strain as well as a construction method and application thereof. The influenza virus sensibilization cell strain STX-TMP2 can stably express a human-derived influenza virus receptor ST6GAL1 and utilizes doxycycline to controllably induce a TMPRSS2 protein. The invention also discloses a preparation method of the influenza virus sensibilization cell strain STX-TMP2. The preservation number of the cell strain STX-TMP2 is GDMCC (China General Microbiological Culture Collection Center) NO: 67099; the cell strain has the following advantages: low toxicity: low background expression is realized through a Tet-on system, cytotoxicity caused by continuous high expression is significantly reduced, and long-term stable passage of the cell strain is ensured; controllability: the doxycycline can regulate and control the expression level and flexibly adapt to the production requirements of influenza virus vaccine strains; high efficiency: in an induction state, the cells have enough receptor expression and serine protease activity at the same time, and the amplification efficiency of the codon reprogramming virus with relatively low replication capacity is remarkably improved; wide applicability: the method is not only suitable for production of influenza virus vaccine strains with codon reprogramming, but also can be popularized to various production systems of attenuated influenza vaccines with relatively low replication ability.