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Virus receptor protein for diagnosing leucoderma and application thereof

A receptor protein and vitiligo technology, applied in the field of biomedicine, can solve problems such as inconsistent results, achieve higher diagnostic accuracy, uncomplicated detection process, and reduce the risk of disease progression

Pending Publication Date: 2020-10-30
THE FIRST HOSPITAL OF CHINA MEDICIAL UNIV
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  • Claims
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Problems solved by technology

However, there are two other studies showing that there is no significant difference in HSV DNA and serum HSV antibodies in serum and skin tissue between patients with vitiligo and normal controls.
The results of the above-mentioned human specimens are inconsistent with those of mice. The possible reasons for the analysis are as follows: the amount of virus or its antibodies in serum or skin lesions is small, not enough to be detected or the timing of positive detection has not been caught; the effect of HSV on human melanin was not directly detected. The influence of cells; the level of HSV itself does not change, but the level of its receptor changes or other mechanisms initiated by HSV are involved in the damage of melanocytes; a process is required from the damage of melanocytes caused by HSV to the formation of vitiligo
[0006] HSV-1 receptor as a molecular marker for diagnosis of vitiligo has not been reported

Method used

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  • Virus receptor protein for diagnosing leucoderma and application thereof
  • Virus receptor protein for diagnosing leucoderma and application thereof
  • Virus receptor protein for diagnosing leucoderma and application thereof

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Embodiment Construction

[0022] The present invention will be further elaborated below in conjunction with specific embodiments and accompanying drawings.

[0023] Example.

[0024] 1. Experimental materials and grouping.

[0025] 1. Materials: cell line and HSV-1 virus.

[0026] The immortalized human epidermal melanocyte line PIG1 and the immortalized human vitiligo melanocyte line PIG3V were obtained from the Department of Dermatology, Xijing Hospital, Fourth Military Medical University, respectively. Cultures were cultured in Medium 254 (Gibco) containing human melanocyte growth supplement HMGS (Gibco), 10% fetal bovine serum (FBS) (Cellmax) and 1% penicillin-streptomycin antibiotic mixture (Bioindustries). The STR-identified human cervical cancer cell line HeLa was purchased from iCell Bioscience Inc (Shanghai, China) and cultured in DMEM (Hyclone) containing 10% fetal bovine serum and 1% penicillin-streptomycin. All cell lines were stored at 37°C, 5% CO 2 incubated in a humidified incubator....

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Abstract

The invention belongs to the technical field of biological medicines, and particularly relates to a virus receptor protein for diagnosing leucoderma and application thereof. The invention discloses application of a molecular marker in preparation of a diagnostic kit for diagnosing leucoderma. The molecular marker is human skin melanocyte HSV-1 receptor protein. The human skin melanocyte HSV-1 receptor protein is determined by using a western blot method to carry out semi-quantitative analysis and predict whether the protein level is reduced or not. Experiments prove that after HSV-1 infects human melanocytes, expression of melanin synthesis related protein and a melanin synthesis function are inhibited by up-regulating VN1R5, so that leucoderma is caused. The process of the human skin melanocyte HSV-1 receptor protein is not complex, the detection result can be obtained by using a microplate reader, and the kit is mature in manufacturing means, easy to purchase and capable of being popularized in clinical application. It is found in the early stage that the reduced human skin melanocyte HSV-1 receptor protein level is beneficial to diagnosis of leucoderma, guidance of next treatment is facilitated, and the risk of disease deterioration of leucoderma patients is reduced.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a virus receptor protein for diagnosing vitiligo and its application. Background technique [0002] Vitiligo (vitiligo) is an acquired skin or mucous membrane depigmentation skin disease characterized by loss of functional melanocytes and skin / mucosal melanin. There are obvious differences in the prevalence of vitiligo in different regions and different ethnic groups, generally about 0.1%-2.9%. Vitiligo is a recurrent and multifactorial related skin disease, which often brings great psychological pressure to patients and their families. Therefore, early diagnosis and active treatment of vitiligo are very important to improve the prognosis of patients. [0003] So far, the etiology and pathogenesis of vitiligo are still unclear, and different scholars have put forward a variety of theories, including autoimmunity theory, genetic theory, neurochemical factor theor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2333/705G01N2800/205
Inventor 吴严齐瑞群冯雨欣乔帅苑金平孙艳肖碧环高兴华陈洪铎
Owner THE FIRST HOSPITAL OF CHINA MEDICIAL UNIV
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