Virus receptor protein for diagnosing leucoderma and application thereof
A receptor protein and vitiligo technology, applied in the field of biomedicine, can solve problems such as inconsistent results, achieve higher diagnostic accuracy, uncomplicated detection process, and reduce the risk of disease progression
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[0022] The present invention will be further elaborated below in conjunction with specific embodiments and accompanying drawings.
[0023] Example.
[0024] 1. Experimental materials and grouping.
[0025] 1. Materials: cell line and HSV-1 virus.
[0026] The immortalized human epidermal melanocyte line PIG1 and the immortalized human vitiligo melanocyte line PIG3V were obtained from the Department of Dermatology, Xijing Hospital, Fourth Military Medical University, respectively. Cultures were cultured in Medium 254 (Gibco) containing human melanocyte growth supplement HMGS (Gibco), 10% fetal bovine serum (FBS) (Cellmax) and 1% penicillin-streptomycin antibiotic mixture (Bioindustries). The STR-identified human cervical cancer cell line HeLa was purchased from iCell Bioscience Inc (Shanghai, China) and cultured in DMEM (Hyclone) containing 10% fetal bovine serum and 1% penicillin-streptomycin. All cell lines were stored at 37°C, 5% CO 2 incubated in a humidified incubator....
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