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Molecular marker for diagnosing leucoderma diseases and application of thereof

A technology of molecular markers and vitiligo, applied in the field of biomedicine, to reduce the risk of disease exacerbation and improve the accuracy of diagnosis

Pending Publication Date: 2020-11-03
THE FIRST HOSPITAL OF CHINA MEDICIAL UNIV
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Problems solved by technology

Human skin melanocyte VN1R5 protein as a molecular marker for the diagnosis of vitiligo has not been reported

Method used

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  • Molecular marker for diagnosing leucoderma diseases and application of thereof
  • Molecular marker for diagnosing leucoderma diseases and application of thereof
  • Molecular marker for diagnosing leucoderma diseases and application of thereof

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Embodiment Construction

[0021] The present invention will be further elaborated below in conjunction with specific embodiments and accompanying drawings.

[0022] Example.

[0023] 1. Experimental materials and grouping.

[0024] 1. Materials: cell line and HSV-1 virus.

[0025] The immortalized human epidermal melanocyte line PIG1 and the immortalized human vitiligo melanocyte line PIG3V were obtained from the Department of Dermatology, Xijing Hospital, Fourth Military Medical University, respectively. Cultures were cultured in Medium 254 (Gibco) containing human melanocyte growth supplement HMGS (Gibco), 10% fetal bovine serum (FBS) (Cellmax) and 1% penicillin-streptomycin antibiotic mixture (Bioindustries). The STR-identified human cervical cancer cell line HeLa was purchased from iCell Bioscience Inc (Shanghai, China) and cultured in DMEM (Hyclone) containing 10% fetal bovine serum and 1% penicillin-streptomycin. All cell lines were stored at 37°C, 5% CO 2 incubated in a humidified incubator....

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Abstract

The invention belongs to the technical field of biological medicines, and particularly relates to a molecular marker for diagnosing leucoderma and application thereof. The invention discloses application of a molecular marker in preparation of a diagnostic kit for diagnosing leucoderma. The molecular marker is human skin melanocyte VN1R5 protein. The human skin melanocyte VN1R5 protein is determined by using a western blot method to carry out semi-quantitative analysis, and whether the protein level is increased or not is predicted. Experiments prove that after HSV1 infects human melanocytes,expression of melanin synthesis related protein and a melanin synthesis function are inhibited by up-regulating VN1R5, so that leucoderma is caused. The human skin melanocyte VN1R5 protein detection process is not complex, the detection result can be obtained by using a microplate reader, and the kit is mature in manufacturing means, easy to purchase and capable of being popularized in clinical application. The founding of the increased human skin melanocyte VN1R5 protein level in the early stage, is beneficial to diagnosis of leucoderma and guidance of next treatment, and the risk of diseasedeterioration of leucoderma patients is reduced.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a molecular marker for diagnosing vitiligo and its application. Background technique [0002] Vitiligo (vitiligo) is an acquired skin or mucous membrane depigmentation skin disease characterized by loss of functional melanocytes and skin / mucosal melanin. There are obvious differences in the prevalence of vitiligo in different regions and different ethnic groups, generally about 0.1%-2.9%. Vitiligo is a recurrent and multifactorial related skin disease, which often brings great psychological pressure to patients and their families. Therefore, early diagnosis and active treatment of vitiligo are very important to improve the prognosis of patients. [0003] So far, the etiology and pathogenesis of vitiligo are still unclear, and different scholars have put forward a variety of theories, including autoimmunity theory, genetic theory, neurochemical factor theory, oxi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2800/20
Inventor 吴严齐瑞群冯雨欣乔帅苑金平孙艳肖碧环高兴华陈洪铎
Owner THE FIRST HOSPITAL OF CHINA MEDICIAL UNIV
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