Molecular marker for diagnosing anti-MDA5 positive dermatomyositis and application thereof

A molecular marker and dermatomyositis technology, applied in the field of biomedicine, can solve the problems of difficult selection of kit standard products, popularization, and complex detection methods of anti-MDA5 antibodies, so as to reduce the risk of disease progression and death and improve diagnostic accuracy Good results

Pending Publication Date: 2022-01-21
THE FIRST HOSPITAL OF CHINA MEDICIAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The study of the present invention found that the levels of serum soluble ICAM-1 and VCAM-1 in patients with dermatomyositis were higher than those of normal people, and the serum levels of the two in anti-MDA5 antibody-positive dermatomyositis were higher than those in the anti-MDA5 antibody-negative group, and serum soluble intercellular adhesion molecules -1 ICAM-1 and vascular cell adhesion molecule-1 VCAM-1 are used as markers for diagnosing anti-MDA5 positive dermatomyositis, which solves the complex detection methods of anti-MDA5 antibodies in the prior art, the difficulty in making kits and selecting standard products, Immunoprecipitation method is complicated to operate and cannot be popularized in clinical applications

Method used

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  • Molecular marker for diagnosing anti-MDA5 positive dermatomyositis and application thereof
  • Molecular marker for diagnosing anti-MDA5 positive dermatomyositis and application thereof
  • Molecular marker for diagnosing anti-MDA5 positive dermatomyositis and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0018] Example 1 Detection of serum levels of soluble ICAM-1 and VCAM-1 in patients with dermatomyositis.

[0019] From October 2017 to December 2019, 45 patients with dermatomyositis were treated in the Department of Rheumatology and Immunology, the First Affiliated Hospital of China Medical University. According to the presence or absence of anti-MDA5 antibodies, they were divided into anti-MDA5 positive dermatomyositis (25 cases) and anti-MDA5 Negative dermatomyositis (20 cases). During the same period, there were 21 healthy controls matched by sex and age. All research subjects collected 5 ml of venous blood at the time of selection, added it to a vacuum glass tube without anticoagulant, centrifuged at 3000 r / min for 10 min, separated serum samples, and tested serum samples using enzyme-linked immunosorbent assay (ELISA) kits. The levels of sICAM-1 and sVCAM-1 were strictly operated according to the instructions of the reagents. The operation steps are as follows, add 10...

Embodiment 2

[0020] Example 2 Serum soluble adhesion molecules can be quantitatively analyzed by enzyme-linked immunosorbent assay and the presence or absence of anti-MDA5 antibodies can be predicted.

[0021]The predictive value of the serum sICAM-1 and sVCAM-1 levels detected in Example 1 for anti-MDA5 antibody-positive dermatomyositis was analyzed by drawing a ROC curve. Application of SPSS 20.0 and MedCalc 17.0 for drawing and statistical analysis. Binomial Logistic regression was used to output the predicted probabilities of sICAM-1, sVCAM-1 and sICAM-1 combined with sVCAM-1, and then the three predicted probabilities were analyzed with the anti-MDA5 antibody by ROC curve. The cut-off value, sensitivity and specificity of serum sICAM-1, sVCAM-1 and sICAM-1 combined with sVCAM-1 in the diagnosis of anti-MDA5 positive dermatomyositis were analyzed. An area under the curve between 0.5-0.7 indicates low diagnostic value; between 0.7-0.9 indicates medium diagnostic value; above 0.9 indica...

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Abstract

The invention belongs to the technical field of biological medicines, and particularly relates to a molecular marker for diagnosing anti-MDA5 positive dermatomyositis and application thereof. The invention relates to an application of a molecular marker in preparation of a diagnostic kit for diagnosing anti-MDA5 positive dermatomyositis. The molecular marker is a serum soluble intercellular adhesion molecule-1 (ICAM-1) and a vascular cell adhesion molecule-1 (VCAM-1). Experiments prove that ICAM-1 and VCAM-1 levels of dermatomyositis patients are higher than those of normal people, the levels of anti-MDA5 antibody positive dermatomyositis serum of the ICAM-1 and VCAM-1 are higher than those of anti-MDA5 antibody negative groups, ICAM-1 and VCAM-1 serve as markers for diagnosing anti-MDA5 positive dermatomyositis, and the problems that in the prior art, an anti-MDA5 antibody detection method is complex, kit manufacturing and standard substance selection are difficult, an immunoprecipitation method is complex in operation, and the detection method cannot be popularized in clinical application. The early detection of the increased adhesion molecule level is beneficial to diagnosis of anti-MDA5 antibody positive dermatomyositis, guidance of next treatment is facilitated, and the risk of disease deterioration and death of dermatomyositis patients is reduced.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a molecular marker for diagnosing anti-MDA5 positive dermatomyositis and its application. Background technique [0002] Idiopathic inflammatory myopathy (Idiopathic inflammatory myopathy, IIM) is a systemic disease that mainly affects skeletal muscle, and its clinical features are skeletal muscle weakness, skin lesions, systemic organ damage and the production of specific antibodies. Polymyositis (PM) and dermatomyositis (DM) are the two most common types. Interstitial lung disease (ILD) is a common complication of PM / DM and the main cause of death in PM / DM patients, with a mortality rate of about 50%. Among them, rapidly progressive interstitial lung disease (rapidlyprogressive interstitial lung disease, RP-ILD) has a higher mortality rate, accounting for about 50-75%. RP-ILD is defined as acute interstitial pneumonia with dyspnea or worsening cough over weeks ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/58G01N33/543
CPCG01N33/6893G01N33/581G01N33/54306G01N2333/70525G01N2800/20G01N2800/10
Inventor 杨娉婷杨春姝林美艺
Owner THE FIRST HOSPITAL OF CHINA MEDICIAL UNIV
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