The invention relates to high-encapsulation-rate wild
cactus polysaccharide gelatin microspheres and a preparation method thereof. The preparation method comprises the steps that: a small amount of
gelatin is dissolved into a proper amount of water; a wild
cactus polysaccharide solution is added; the materials are sufficiently mixed and pre-emulsified, such that a water-phase solution is prepared; an oil-phase continuous phase is
liquid paraffin, and a small amount of tween-80 is added as an emulsifier; on a basis that original stable condition is maintained, the
gelatin polysaccharide water phase is slowly added into
liquid paraffin, and the mixture is well mixed; after sufficient emulsification, the mixture is rapidly cooled to below 5 DEG C while stirring is continued;
glutaraldehyde is added, and curing is carried out for 2h;
centrifugation is carried out and precipitate is fetched;
residual oil and
glutaraldehyde are removed by washing by using isopropanol or
ethanol; and lyophilization is carried out, such that microspheres are obtained. With the method,
drug load and encapsulation rate of wild
cactus polysaccharide microspheres respectively reaches 7.47% and 91.38%. The method has the advantages of simple process, low cost, and high
repeatability. The prepared wild cactus polysaccharide
gelatin microspheres has round appearance, uniform particle size, good dispersion, high encapsulation rate, good
biocompatibility, and good biodegradability.