The invention provides a preparation method of a feed-grade zinc oxide premixing agent. The preparation method comprises the following steps of (1) grinding zinc oxide powder, performing screening with an 80-mesh sieve to obtain fine powder, adding the fine powder into an ethanol water solution, dropwise adding a silane coupling agent solution, and taking suspension liquid for standby application; (2) adding a delta-tocopherol-ethanol solution to the suspension liquid under the normal atmospheric temperature, performing heating to 60-70 DEG C, performing a reaction for 2-3 hours, taking suspension liquid, performing filtering, washing filter cakes with ethanol, performing drying, performing grinding, and performing screening so as to obtain nanometer zinc oxide; (3) mixing the nanometer zinc oxide with starch, sodium hydroxypropycellulose, sodium cyclamate, a binder and a wetting agent through a mixing machine, performing granulation, performing shot blasting, and performing drying to obtain mini pills; and (4) performing screening on the mini pills with a classifying sieve so as to obtain the feed-grade zinc oxide premixing agent of which the particle size is 20-80 meshes. The zinc oxide particles in the made zinc oxide premixing agent are dispersed in a nanometer grade, so that the feed-grade zinc oxide premixing agent is uniform to disperse and not liable to agglomerate, has favorable biological value and utilization, and cannot generate side effects on growth of animals.