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145results about How to "Round appearance" patented technology

Preparation method of antimicrobial peptide slow-release microcapsules

ActiveCN102824332ADoes not cause toxic residueAvoid the influence of embedded antimicrobial peptidesAntibacterial agentsPeptide/protein ingredientsCrystallographyMicroparticle
The invention discloses a preparation method of antimicrobial peptide slow-release microcapsules and relates to a preparation method of microcapsules. The invention solves the problems that a preparation mode of the existing chitosan / sodium alginate microcapsules is only suitable for laboratories or the small-scale production, the industrial large-scale production is difficult to form and the existing antimicrobial peptide is short of an effectively slow-release envelope. The preparation method comprises the following steps of: 1, uniformly mixing aqueous solution of sodium alginate and chitosan acid solution by a high-speed homogeneous machine to form slow-release particles; and 2, adding the antimicrobial peptide and the slow-release particles into a medical mixer together to carry out embedding to obtain antimicrobial peptide particles and then carrying out beta-cyclodextrin embedding on the antimicrobial peptide particles to obtain the antimicrobial peptide slow-release microcapsules. The preparation method can be used for producing an antimicrobial peptide medicament and has a very large development space in the field of medicine.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY

Preparation method for particle compound fertilizer

The invention relates to a preparation method for a particle compound fertilizer. The method comprises the following steps of: wetting or heating and mixing a basic fertilizer and a filler to obtain a plastically-enhanced material with high moisture content or high temperature; preparing a mixed material into uniform particles through extruding machinery; rounding the surfaces of particle materials; drying (e.g., the step can be eliminated when the temperature for preparing the particle materials is high); and cooling and sieving to obtain the particle compound fertilizer. Compared with the conventional extruding method, the method has the advantage that: abrasion on extruding equipment is reduced. Compared with the conventional agglomerating method, the method has the advantages that: material returning multiple in a production process can be reduced, and production energy consumption and production cost are lowered. Product particles are uniform, are round in appearance, and have high pressure resistance and agglomeration resistance. In production process, waste emission is reduced, and the field operation environment is remarkably improved.
Owner:SHANGHAI RES INST OF CHEM IND

Monoclonal antibody of anti lymphocyst virus of and preparation method

Two monoclonal antibodies B and C for the lymphocystics virus of bastard halibut are prepared from two hybrid tumor cells (CCTCC-C200419 and CCTCC-C200420) through conventional culture for 2-3 days to secrete a lot of said monoclonal antibodies B and C.
Owner:OCEAN UNIV OF CHINA

Monoclonal antibody of immunoglobulin of anti lymphocyst vitos of Pacific fluke, and preparation method

This invention discloses monoclonal antibody against anti-LCDV immunoglobulin of Paralichthys olivaceus, which is excreted by hybridoma JF-lgM-H (CCTCC-C200631). The method comprises: immunizing Paralichthys olivaceus with LCDV inactivated by formalin to prepare antiserum, purifying Paralichthys olivaceus immunoglobulin, immunizing Balb / c mice as antigen, preparing hybridoma cells by cell engineering method, and screening the monoclonal antibody by immunoassay. Indirect ELISA and indirect immunofluorescent antibody assay show that this monoclonal antibody is located on the heavy chain (7-80 kDa) of the anti-LCDV immunoglobulin. The monoclonal antibody can be used for preparing reagents for detecting LCDV infection in early stage, and evaluating the immune effects of LCDV vaccine inactivated by formalin.
Owner:OCEAN UNIV OF CHINA

Formula of aureomycin hydrochloride premix granule and granulating process

The invention discloses a formula of aureomycin hydrochloride premix granule, which is characterized in that the following components are included: 80 percent or less than 80 percent of aureomycin hydrochloride, 80 percent or less than 80 percent of soluble starch or starch degradation products, 20 percent or less than 20 percent of carbohydrates, and 30 percent or less than 30 percent of purified water. The invention further discloses a granulating process. After the adoption of the formula and the granulating process, the invention achieves that the aureomycin hydrochloride premix granule is uniform, has a mellow appearance, is unanimous in tincture and high in percentage of ripened grains, the stability of product quality is excellent, the normal working conditions during the aureomycin hydrochloride premix granulating process can be ensured, and the improvement to production efficiency and quality is facilitated.
Owner:PUCHENG CHIA TAI BIOCHEM

Embolic microspheres containing ion exchange functional groups

The invention discloses a preparation method of embolic microspheres containing ion exchange functional groups. According to the preparation method, AMPS (2-acrylamide-2-methylpro panesulfonic acid) is prepared into a mild crosslinking prepolymer and then blended with SA (sodium alginate), a PAMPS and SA polymer blend solution is added into liquid paraffin, interpenetrating polymer network microspheres prepared with a reversed phase suspension crosslinking method are in regular spherical shape, the particle sizes range between 300 microns and 700 microns in a concentrated manner, the drug loading rate of the microspheres can be 25%-45%, the performance after high temperature sterilization and sealing is stable, and the embolic microspheres have application prospect in anti-tumor embolism chemotherapy.
Owner:嘉兴嘉湖骨科医院管理有限公司

Compound turmeric lipid cubic liquid crystalline nano-particles and preparation method thereof

The invention provides compound turmeric lipid cubic liquid crystalline nano-particles and a preparation method thereof. The compound turmeric lipid cubic liquid crystalline nano-particles comprise the following components in parts by weight: 0.05-0.15g of phytantriol, 20-30mg of F127, 5-7mg of turmeric, 1-3mg of piperine and 15-25mL of water. Compared with a raw medicine group consisting of turmeric and piperine, the compound turmeric lipid cubic liquid crystalline nano-particles provided by the invention are significantly improved to show the properties that the in-vitro stability and solubility of turmeric can be improved on the one hand, the in-vivo bioavailability of turmeric can be significantly improved on the other hand, slow release and targeting effects can also be achieved, and the curative effects of turmeric on therapy in vivo can be enhanced so as to lay a foundation for turmeric to play the extensive pharmacological effects of resisting tumor, improving cardiovascular functions, resisting inflammation, resisting virus, protecting the liver and enhancing the immunity.
Owner:崔景朝

Production process of urea formaldehyde synergistic compound fertilizer

The invention discloses a production process of a urea formaldehyde synergistic compound fertilizer. According to the production process, bentonite compound hydrogel packaging urea, diammonium phosphate and potassium chloride is taken as a solid material, urea formaldehyde is sprayed to the solid material for granulation, charcoal and a microbial agent are added in powder applying manner, so thatthe double-control release of a nitrogen fertilizer and the controllable release of a phosphorus fertilizer and a potassium fertilizer can be realized, and the activity of the microbial agent is improved. Compared with existing products, the product prepared by virtue of the production process provided by the invention has the advantages that the particle size is uniform, dust is little, the appearance is mellow and full, the agglomeration does not easily occur, nutrients are uniformly released, the period is long, the loss does not easily occur, the complete release is realized, the utilization rates of fertilizers N, P and K are increased, and the relatively high validity of the nutrients including nitrogen, phosphorus and potassium is maintained in each growth period of crops; and the product can be completely decomposed by microorganisms, does not have residue and is environmentally friendly, capable of improving microbial florae of soil and preventing hardening and accordant withthe requirements on the development of green ecological agriculture.
Owner:拉多美(宁陵)化肥有限公司

Phytase-coated pellets and preparation method thereof

The invention relates to phytase-coated pellets and a preparation method thereof. Each pellet comprises a phytase-containing pellet core and a coating layer wrapped on the same. The phytase-containing pellet core is made of phytase, starch, microcrystalline cellulose, calcium carbonate, sodium sulfate, ammonia chloride, dried cotton steep liquor powder and polysorbate 80. The coating layer is made of polyacrylic resin, hydroxypropyl methyl cellulose, hydrogenated oil, talc powder and dextrin. The phytase-coated pellets are round and smooth and uniform in particle size, particles in 20-40 meshsize are more than 99%, and volume weight of the phytase-coated pellets is 800g / L-900g / L, so that the phytase-coated pellets can highly uniformly mixed with fodder. In addition, the phytase-coated pellets are highly stable and can be stored at normal temperature 25 DEG C for 6 months, and enzyme activity loss is about 3%-5%. Further, the phytase-coated pellets are highly heat-resistant, and retention rate of the phytase-coated pellets can reach 92%-95% under conditions of fodder conditioning temperature more than 85 DEG C, conditioning time 18-40s, and steam pressure 0.6-0.7Mpa.
Owner:QINGDAO VLAND BIOTECH GRP +1

Multi-drawing-wire conductor wire-stranding method

The invention discloses a multi-drawing-wire conductor wire-stranding method. The method includes the working procedure of wire drawing and the working procedure of wire stranding. According to the wire drawing working procedure, a plurality of metal wires are formed by drawing on a multi-wire drawing machine in a one-time mode, and the formed metal wires are combined into a strand of metal wires which is wound on a bobbin; according to the wire stranding working procedure, a plurality of strands of metal wires are stranded on a wire stranding machine in a one-time mode, and the strands of metal wires after the stranding are combined into a metal conductor which is wound on a bobbin. The product produced with the method is round and normal in appearance, good in flexibility and reduced in outer diameter, and the method saves raw material and labor, reduces production field area and energy consumption and promotes production efficiency.
Owner:GUILIN INT ELECTRIC WIRE & CABLE GROUP

Granular quinocetone premix and preparation method thereof

The invention discloses a granular quinocetone premix which comprises the following components in parts by weight: 5 to 50 parts of quinocetone, 41 to 89 parts of montmorillonite, 2 to 4 parts of sodium cyclamate and 3 to 5 parts of bonder. A preparation method for the granular quinocetone premix comprises the following steps of: uniformly mixing the components and a wetting agent through a mixing machine, then pelletizing by a squeezing granulator, shot blasting by a spherical shot blasting machine, sieving and drying to obtain the granular quinocetone premix with granularity of 20 to 80 meshes. The quinocetone premix is uniform in granules, mellow and full in appearance, accordant in color and luster, good in fluidity and stable in product quality and can be mixed with feed uniformly as well as has better effects on diarrhea prevention and growth promotion of beasts and birds; the preparation method for the granular quinocetone premix has the advantages of high granulation rate, environment-friendliness, dust free and capability of effectively avoiding cross infections.
Owner:ZHEJIANG ESIGMA BIOTECH CO LTD

Method for producing composite fertilizer needed no drying

InactiveCN101077844ARaise the granulation temperatureHigh ball rateFertiliser formsFertilizer mixturesGaseous ammoniaChemistry
The complex fertilizer producing process without need of drying step includes the following steps: pelletizing the solid material in a pelletizing machine, spraying 60-98 wt % concentration sulfuric acid solution into the pelletized material, adding liquid ammonia or gaseous ammonia for reaction to release heat for pelletizing, cooling and sieving to obtain pelletized complex fertilizer product. The process has the reaction heat well utilized, and has high pelletizing temperature, high balling degree, raised production capacity, lowered cost and saving in power.
Owner:SHANGHAI RES INST OF CHEM IND

Method for preventing and treating citrus yellow shoot

The invention discloses a method for preventing and treating citrus yellow shoot. The method includes the steps: cleaning an orchard in winter; spraying normal medicines for killing citrus psylla; spraying medicine compositions for preventing the citrus yellow shoot for 1-3 times before citrus summer shoot germination every year. By the method for preventing and treating the citrus yellow shoot, yield and sugar content can be increased, citruses are good in taste, mellow and full in appearance and high in quality, and use effects of the medicine compositions are higher than those of conventional medicine compositions for preventing the citrus yellow shoot.
Owner:钟山佳欣果蔬有限责任公司

Dry optical fiber ribbon loose tube air blowing molding device

The invention relates to a dry optical fiber ribbon loose tube air blowing molding device. The air blowing molding device is characterized by comprising a plastic extruding device and an air blowing device; the air blowing device includes an optical fiber ribbon guide pipe, a front positioning end cover, an air blowing device tank, a back positioning end cover and an air supply component connected with the air blowing device tank; the front positioning end cover and the back positioning end cover are respectively arranged at two ends of the air blowing device tank; and the optical fiber ribbon guide pipe penetrates through the whole air blowing device tank. The device is skillful in whole structural design, can satisfy the production of both optical fiber small sleeves and optical fiber ribbon large sleeves, and is convenient for disassembly and assembly.
Owner:NANJING WASIN FUJIKURA OPTICAL COMM LTD

Method for manufacturing enhanced flat cable for wagon dumper

The invention discloses a method for manufacturing an enhanced flat cable for a wagon dumper, and the method is characterized by comprising the following steps: material selection, wire drawing, conductor twisting, winding and wrapping of an isolating layer, extrusion of the insulating layer, cabling of wiring harness, primary shield of wiring harness, secondary shield of wiring harness, extrusion of an outer sheath, performance detection and finished products. In the method, the conductor center of the cable is provided with a steel wire rope stiffener so as to improve the longitudinal stretching resistance of the cable; and a wire braid is used as a metal shielding layer, the primary shielding braid density is not less than 80%, and the secondary shielding braid density is not less than85%, thus not only improving shielding factors of a cable core but also providing effective physical and mechanical protective actions for the cable. The cable manufactured by the method has the advantages of good flexibleness, salt mist resistance, greasy dirt resistance and inflaming retarding property, no cracking and no deformation for long term use, and no core breaking at the movable operating mode speed of 1.2m / s.
Owner:SICHUAN MINGXING CABLE

Method for producing compound fertilizer by extrusion-kneading process

The invention relates to a method for producing a compound fertilizer by an extrusion-kneading process, which comprises the following steps: (1) plasticizing a raw material mixture; (2) extruding the plasticized raw material mixture into strips, or pressing into sheets and cutting into strips; and (3) sending the strips into an extrusion-kneading granulation device to form compound fertilizer granules. Compared with the prior art, the product obtained by the method has the advantages of uniform granular size, smooth appearance, more accurate nutrients and improved storage quality. Compared with the traditional pellet process, the method provided by the invention further simplifies the production process, can save the steps of drying, screening, large granule crushing and the like, obviously reduces the exhaust emission, obviously improves the production shop environment and greatly lowers the comprehensive energy consumption.
Owner:SHANGHAI RES INST OF CHEM IND

Method for preparing spherical molecular sieve

The invention relates to a method for preparing a spherical molecular sieve. The method comprises the steps: (1) preparation of small-particle nuclei: adding a molecular sieve fine powder raw material and water to a stirring granulator, and performing stirring and granulation so as to obtain the small-particle nuclei with a particle diameter of 0.5-2.0 mm; (2) preparation of molecular sieve spherical particles: adding the small-particle nuclei, the molecular sieve fine powder raw material and water to a coating granulator so as to form the molecular sieve spherical particles; and (3) preparation of the finished product: performing sieving, drying and calcination on the molecular sieve spherical particles so as to obtain the spherical molecular sieve product. Compared with the prior art, the method has the advantages of a high pelletization rate of nuclei, energy saving, environmental protection and a simple preparation process, and the molecular sieve particles are compact, and has a rounded appearance and smooth appearance.
Owner:SHANGHAI RES INST OF CHEM IND

Monoclonal antibody capable of resisting surface marker molecule CD4-2 of T cell of paralichthys olivaceus as well as preparation method and application of monoclonal antibody

ActiveCN108484769AHealth status can be judgedAssess the level of immune responseImmunoglobulins against cell receptors/antigens/surface-determinantsImmunoassaysFluorescenceEnzyme
The invention discloses a monoclonal antibody capable of resisting a surface marker molecule CD4-2 of a T cell of paralichthys olivaceus. The monoclonal antibody is secreted by a hybridoma cell whichis named by hybridoma cell JF-CD4-2-1D8 and collected in the China Center for Type Culture Collection, with the collection number of CCTCC NO: C201880, in Wuhan University in Wuhan, Hubei, on March 29. 2018. An experimental result of indirect enzyme-linked immunosorbent assay of the monoclonal antibody shows that the monoclonal antibody can be specifically combined with CD4-2 polypeptide; an immunoblotting result shows that the monoclonal antibody disclosed by the invention can specifically identify the paralichthys olivaceus CD4-2 recombinant protein with the molecular weight of 25kDa and theparalichthys olivaceus natural CD4-2 protein with the molecular weight of about 33 kDa; indirect immunofluorence and flow cytometry show that the monoclonal antibody can specifically identify CD4-2 molecules on the surface of lymphocyte of the paralichthys olivaceus. As the monoclonal antibody capable of resisting the surface marker molecule CD4-2 of the T cell of the paralichthys olivaceus, an important tool is provided for detection of paralichthys olivaceus CD4-2+T lymphocyte; the monoclonal antibody has an important practical significance for deep understanding of immunologic mechanisms of fish cells and development of an efficient and environment-friendly fish vaccine.
Owner:OCEAN UNIV OF CHINA

Tumor targeting nanometer micelle, preparation method and application of tumor targeting nanometer micelle as drug carrier

The invention particularly relates to a tumor targeting nanometer micelle, a preparation method and an application of the tumor targeting nanometer micelle as a drug carrier. Aiming at the defect thata traditional chemotherapy method is poor in selectivity, the invention aims to design a drug delivery carrier having the multifunctional targeting effect and an acoustodynamic effect and improve thedelivery efficiency of antitumor drugs. Based on the technical purpose, the invention provides a multifunctional targeting nanometer drug delivery system taking chondroitin sulfate as a skeleton, water-soluble chondroitin sulfate as a basic skeleton, and rhein and lipoic acid as hydrophobic modifications, and the formed nanometer micelle is relatively small in size and has a good drug loading effect and encapsulation efficiency. Disulfide bonds on the surface of the nanometer micelle can be specifically broken after reaching a tumor site, so that positioned release is achieved; and under thestimulation of ultrasonic waves, the nanometer micelle can promote the ability of the rhein to generate active oxygen, so that the utilization rate of the antitumor drugs is effectively increased. Thetumor targeting nanometer micelle provided by the invention is used as an anti-tumor drug carrier and has a good application prospect.
Owner:SHANDONG UNIV

High-encapsulation-rate wild cactus polysaccharide gelatin microspheres and preparation method thereof

The invention relates to high-encapsulation-rate wild cactus polysaccharide gelatin microspheres and a preparation method thereof. The preparation method comprises the steps that: a small amount of gelatin is dissolved into a proper amount of water; a wild cactus polysaccharide solution is added; the materials are sufficiently mixed and pre-emulsified, such that a water-phase solution is prepared; an oil-phase continuous phase is liquid paraffin, and a small amount of tween-80 is added as an emulsifier; on a basis that original stable condition is maintained, the gelatin polysaccharide water phase is slowly added into liquid paraffin, and the mixture is well mixed; after sufficient emulsification, the mixture is rapidly cooled to below 5 DEG C while stirring is continued; glutaraldehyde is added, and curing is carried out for 2h; centrifugation is carried out and precipitate is fetched; residual oil and glutaraldehyde are removed by washing by using isopropanol or ethanol; and lyophilization is carried out, such that microspheres are obtained. With the method, drug load and encapsulation rate of wild cactus polysaccharide microspheres respectively reaches 7.47% and 91.38%. The method has the advantages of simple process, low cost, and high repeatability. The prepared wild cactus polysaccharide gelatin microspheres has round appearance, uniform particle size, good dispersion, high encapsulation rate, good biocompatibility, and good biodegradability.
Owner:ZHANJIANG NORMAL UNIV

Genistein positive ion nano lipid carrier and preparation method thereof

The invention discloses a genistein positive ion nano lipid carrier and a preparation method thereof. Every 100ml of nano lipid carrier comprises the following components: 0.01-0.1g of genistein, 0.8-1.5g of solid lipid material, 0.5-1g of liquid lipid material, 0.1-0.2g of PEG (polyethylene glycol) modified liquid lipid material, 2-4g of emulsifier, 0.1-1g of copolymer of methacrylic acid and methacrylate and the balance of water. The prepared genistein positive ion nano lipid carrier is round in appearance and uniform in mass, encapsulation efficiency is more than 85%, and biocompatibility and biodegradability are good. By adopting the genistein positive ion nano lipid carrier, solubility of genistein is improved, in vitro release of genistein is prolonged, and the aims of prolonging intraocular preparation retention time and reducing toxic and side effects of a medicine are achieved.
Owner:SHENYANG PHARMA UNIVERSITY

Light flame-retardant plate shielding flame-retardant composite layer, filling layer and manufacturing method thereof

The invention discloses a light flame-retardant plate shielding flame-retardant composite layer, a filling layer and a manufacturing method thereof. The shielding control cable comprises cable cores and an outer insulated layer coating the cable cores. Multiple conductors are stranded and then coated with an inner shielding layer to form each cable core; an inner insulated layer is arranged outside the inner shielding layer; an outer shielding layer is arranged outside the inner insulated layer; a flame-retardant composite layer is arranged outside the outer shielding layer; multiple cable cores are mutually surrounded and distributed uniformly and circumferentially; an isolation sleeve is arranged outside the multiple cable cores; the isolation sleeve is coated with a filling layer; the filling layer is coated with a protection later; and the outer insulated layer coats the protection layer. In the case of cabling, the cable has a round and even appearance; the mechanical strength is high, the surface sealing performance is good, and advantages of pressure resistance and light weight can be provided; and as the cable also comprises the flame-retardant composite layer formed by a nano material flame-retardant agent and the present flame-retardant agent in a mixing mode, the flame-retardant performance and the heat-resistance performance are achieved, and the fire-resistance time is long.
Owner:ANHUI TIANYUAN CABLE

Azithromycin microball preparation and preparation method thereof

The invention discloses an azithromycin microsphere medicament comprising azithromycin and acceptable carrier material on the pharmacy, which adopts biodegradable gelatin as the carrier material. A gelatin microsphere is prepared through emulsification coagulation, wherein the azithromycin is enwrapped in the gelatin microsphere, thereby diminishing the granularity of the medicament, improving the bioavailability of the medicament, concealing effectively the bitter of the medicament, having stable physicochemical property and good reproducibility and especially facilitating infants to take medicine. The preparation technology of the invention has the advantages of stability and feasibility, low cost and easy reproducibility, which can create giant economic benefit and can be extended as beneficial reference of other medicament preparation technology in the pharmacy industry.
Owner:GUANGDONG PHARMA UNIV

Preparation method of feed-grade zinc oxide premixing agent

The invention provides a preparation method of a feed-grade zinc oxide premixing agent. The preparation method comprises the following steps of (1) grinding zinc oxide powder, performing screening with an 80-mesh sieve to obtain fine powder, adding the fine powder into an ethanol water solution, dropwise adding a silane coupling agent solution, and taking suspension liquid for standby application; (2) adding a delta-tocopherol-ethanol solution to the suspension liquid under the normal atmospheric temperature, performing heating to 60-70 DEG C, performing a reaction for 2-3 hours, taking suspension liquid, performing filtering, washing filter cakes with ethanol, performing drying, performing grinding, and performing screening so as to obtain nanometer zinc oxide; (3) mixing the nanometer zinc oxide with starch, sodium hydroxypropycellulose, sodium cyclamate, a binder and a wetting agent through a mixing machine, performing granulation, performing shot blasting, and performing drying to obtain mini pills; and (4) performing screening on the mini pills with a classifying sieve so as to obtain the feed-grade zinc oxide premixing agent of which the particle size is 20-80 meshes. The zinc oxide particles in the made zinc oxide premixing agent are dispersed in a nanometer grade, so that the feed-grade zinc oxide premixing agent is uniform to disperse and not liable to agglomerate, has favorable biological value and utilization, and cannot generate side effects on growth of animals.
Owner:ZHEJIANG ESIGMA BIOTECH CO LTD

Monoclonal antibody of virus receptor of anti leukoplakia disease and preparation method

A monoclonal antibody B for the white spot virus receptor of prawn is prepared from the hybrid tumor cell (CCTCC-C200418) through conventional culture for 2-3 days to secrete a lot of said monoclonal antibody B. It can be used for preventing and treating whit spot of prawn.
Owner:OCEAN UNIV OF CHINA

Process for producing negative electrode material coke by taking supercritical extraction oil slurry as raw material and application of process

The invention belongs to the technical field of production of negative electrode materials and in particular relates to a process for producing negative electrode material coke by taking supercritical extraction oil slurry as a raw material and application of the process. Before aromatic hydrocarbon-enriched oil is coked, fractional distillation of a fractional distillation tower and a light component removing process are combined; produced raw coke is subjected to drying treatment so that the water content of the raw coke is less than one percent; the process is used for producing the negative electrode material coke; the graphitization degree of a lithium-ion battery prepared by a negative electrode material for preparing the lithium-ion battery reaches 95 percent or more and the capacity reaches 360mAh / g.
Owner:SHANDONG YIDA NEW MATERIAL

Monoclonal antibody for paralichthys olivaceus resistant T cell surface marker molecule CD4-1 as well as preparation method and application thereof

ActiveCN108341875AHealth status can be judgedAssess the level of immune responseBiological material analysisMicroorganism based processesSurface markerFluorescence
The invention discloses a monoclonal antibody for a paralichthys olivaceus resistant T cell surface marker molecule CD4-1. The monoclonal antibody is named as a hybridoma cell JF-CD4-1-2A10, the preservation number is CCTCC NO: C201879, the preservation organization is China Center For Type Culture Collection, the address is Wuhan university of Wuhan city in Hubei province, and the preservation date is March 29th, 2018, and is secreted by the hybridoma cell. An indirect enzyme-linked immunosorbent assay reaction experiment on the monoclonal antibody shows that the monoclonal antibody can be specially combined with paralichthys olivaceus CD4-1 polypeptide, indirect immune fluorescence and flow cytometry prove that the monoclonal antibody can specially identify the paralichthys olivaceus lymphatic cell surface CD4-1 molecule, and an immunoblotting result shows that the monoclonal antibody disclosed by the invention can specially identify paralichthys olivaceus CD4-1 recombinant protein and natural paralichthys olivaceus CD4-1 protein of which the molecular weight is 52 kDa. According to the monoclonal antibody disclosed by the invention, the monoclonal antibody for the paralichthys olivaceus resistant T cell surface marker molecule CD4-1 is prepared, and an important tool is provided for detecting paralichthys olivaceus CD4-1<+T> lymphatic cells.
Owner:OCEAN UNIV OF CHINA

Anti-paralichthys olivaceus immunoglobulin D monoclonal antibody as well as application and preparation method thereof

The invention discloses an anti-paralichthys olivaceus immunoglobulin D monoclonal antibody. The anti-paralichthys olivaceus immunoglobulin D monoclonal antibody is secreted by hybridoma cells of a hybridoma cell strain JF-IgD, with the collection No.: CCTCCNO: C2014159, the collection unit: China Center for Type Culture Collection, the collection address: Wuhan University in Wuhan, Hubei of China, and the collection date: September 2, 2014. Indirect enzyme-linked immune reaction experiment results show that the anti-paralichthys olivaceus immunoglobulin D monoclonal antibody can be specifically bound with recombinant flounder IgD heavy chain proteins; immunoblotting results show that the anti-paralichthys olivaceus immunoglobulin D monoclonal antibody can specifically recognize recombinant protein encoded by flounder IgD heavy chain genes delta1-delta 4 and flounder natural IgD heavy chain proteins with the molecular weight of 120kDa. The anti-paralichthys olivaceus IgD monoclonal antibody is prepared, and an important tool is provided for detecting flounder IgD protein molecules and IgD surface positive (IgD +) lymphocytes.
Owner:OCEAN UNIV OF CHINA

Flat copper wire embedded sheath type shield medium-and-high voltage cable and production process thereof

The invention relates to a flat copper wire embedded sheath type shielding medium and high voltage cable and a production process thereof, belonging to the technical field of power cables. The technical solution is: conductor shielding (2), cross-linked polyethylene insulation (3) and insulation shielding (4) are sequentially arranged outside the conductor core (1), and a plurality of flat copper wires (5) are sparsely wound outside the insulation shielding. The wire is embedded in the outer sheath (6). The conductor core is covered with three layers of co-extruded conductor shield (2), cross-linked polyethylene insulation (3) and insulation shield, and the insulation shield is wrapped with multiple flat copper wires (5). The lower part is embedded with an insulating shield to form a flat copper wire shield, the flat copper wire shield is extruded and wrapped with an outer sheath (6), and the upper part of the flat copper wire is embedded in the outer sheath. The positive effects of the invention: simple structure, excellent performance; flat copper wire shielding overcomes the shortcoming of excessive internal shielding pressure of round copper wires, and at the same time has good structural dimensional stability, higher electrical safety, compact structure, and energy saving Cable costs.
Owner:TANGSHAN HUATONG SPECIAL CABLE MFG CO LTD

Novel pharmaceutical composition for prevention and control of liberobacter asiaticum

The invention discloses a novel pharmaceutical composition for prevention and control of liberobacter asiaticum, wherein the pharmaceutical composition comprises the following raw materials: herba patriniae, pterocarya stenoptera leaves, mint, mangnolia officinalis, melia azedarach, celasturs angulatus maxim, ailanthus altissima, lettuce, sylvite, phosphorus salt, nitrogen salt, calcium salt, magnesium salt, iodised salt, boron salt, molybdenum salt, zinc salt, manganese salt, copper salt, iron salt, vitamins, amino acids, indolebutyric acid, gibberellin, 6-benzylaminopurine, naphthylacetic acid, agar, kinetin, polyvinylpyrrolidone, sodium methylene bis-naphthalene sulfonate, an American DuPont EEA2112AC compatibilizer, an ampullaria gigas powder, alkyl potassium sulfate, acrylate, glycidoxypropyltrimethoxysilane, xanthan gum, and ethanol. The pharmaceutical composition for prevention and control of liberobacter asiaticum can increase the yield and the sugar content; citrus reticulata blanco has the advantages of good taste, mellow and full appearance and color, and high quality; and the pharmaceutical composition has the use effect higher than that of a conventional pharmaceutical composition for prevention and control of liberobacter asiaticum.
Owner:钟山佳欣果蔬有限责任公司
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