Cell line for stable expression of peste des petits ruminants virus receptor Nectin-4 and construction method of cell line
A technology of Peste des petits ruminants, mdbk-nectin-4, applied in the direction of receptors/cell surface antigens/cell surface determinants, viruses/phages, cells modified by introducing foreign genetic material, etc., can solve the problem of direct transfection Low efficiency and other issues, to achieve good genetic stability
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[0035] In order to make the technical means realized by the present invention, creative features, goals and effects easy to understand, the following in conjunction with specific embodiments and appended Figure 1~5 To further illustrate the present invention, the content mentioned in the embodiment is not to limit the present invention. If no specific conditions are indicated in the examples, it shall be carried out according to the conventional conditions or the conditions suggested by the manufacturer. The reagents or instruments used are commercially available routine products, if the manufacturers are not indicated.
[0036] 1. Construction of pLOV-eGFP-Nectin-4 expression vector
[0037] The Nectin-4 gene was synthesized according to the sheep Nectin-4 gene sequence published by Genbank (Accession No. JX944709).
[0038] The Nectin-4 gene sequence (SEQ ID NO.3): (accession number JX944709)
[0039] atgcctctgtccctgggagccgagatgtggggtcctgtggcctggctgctgctgctgctcctggcatcatt...
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