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Application of oxetanocin in preparation of antiviral medicament

A technology of antiviral drug, thetasu, applied in the field of western medicine

Inactive Publication Date: 2013-10-30
江西蓝十字生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although thetasine has been reported, its function is located in antibacterial infection

Method used

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  • Application of oxetanocin in preparation of antiviral medicament
  • Application of oxetanocin in preparation of antiviral medicament
  • Application of oxetanocin in preparation of antiviral medicament

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Experimental program
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Effect test

preparation example Construction

[0041] 2. Virus Preparation and Quantification

[0042] HIV clones were prepared by calcium-precipitating 293T cells. Reagents were purchased from the Profection Kit (www.promega.com) from Promega, USA. See references [2-4] for details. Titration of viral load was determined by measuring the p24 protein of HIV virus. H1N1 Influenza A virus is also used for the preparation of 293T cells by calcium precipitation, see reference [5] . Viruses were quantified by dilution titration. When the virus is diluted to a certain degree, the virus unit when the possibility of virus infecting HOT cells is reduced to 50% is 1 cell culture infection dose 50 (Tissue Culture Infectivity Dose 50, TCID 50 ).

[0043] 3. Virus Infection of Cultured Cells

[0044] Methods for HIV infection of various cells are described in references [2, 4, 6]. To infect HOT cells with influenza A virus, put the cells into a 24-well cell culture dish 16 hours ago. 10 cells per well 4 .Each well contains 0.5...

Embodiment 1

[0047] Embodiment 1 evaluates the antiviral effect of thetasu with the virus with green fluorescent protein

[0048] After CEM-CCR5CD4+, CCR5+ lymphocytes were infected by the same titer of HIV, the virus produced was quantified by the amount of HIV p24 protein. In two days, the amount of virus produced by NL4-3 and the virus with EGFP was equal, indicating that the infection and proliferation rate of HIV with EGFP gene was close to that of wild-type parental virus under the condition of cell culture. At 4 days, the virus produced by NL4-3 had a slight advantage over the virus with EGFP ( image 3 a). The situation of JRCSF infection is also similar ( image 3 b). Therefore, the HIV modified with EGFP gene is suitable for the evaluation research of thetasin antiviral ability.

Embodiment 2

[0049] The inhibition of embodiment 2 thetasu to HIV infection

[0050] We first used the most commonly used combination in the laboratory, NL4-3 HIV to infect HeLa-CD4 cells to measure the anti-HIV effect of thetasu. In order to clarify the therapeutic potential of thetasine as a short peptide drug, we used the short peptide T20 (trade name Fuzeon, produced by Roche) that has been put on the market for the treatment of AIDS as a control to investigate the inhibitory effect of thetasine on HIV infection.

[0051] Different amounts of thetasine or T20 peptide were put into HeLa-CD4 cell culture dishes 1 hour before infection. HeLa-CD4 cells were infected with NL4-3 HIV at an average of 1 virus per cell for 3 hours. Residual virus is then eluted. After 3 days, the cells were collected and put into a flow cytometer to calculate the proportion of infected cells, the results are shown in Figure 4 . Figure 4 It shows that the inhibitory effect of T20 on HeLa-CD4 cells infected...

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Abstract

The invention belongs to the field of Western medicines and discloses an application of oxetanocin in preparation of an antiviral medicament. The application of oxetanocin and derivatives thereof in preparation of antiviral medicaments is preferably used for preparing medicaments for resisting HIV (Human Immunodeficiency Virus) and / or influenza viruses. An antivirus mechanism of oxetanocin is directly related with entrance of the viruses in cells. By analysis on time dynamic expression of oxetanocin, the most possible mechanism of oxetanocin changes flowing of a cell membrane virus receptor. When being put into a cell culture dish before virus infection, the oxetanocin is adsorbed on a cell membrane to restrain the viruses on the cell membrane so that the receptor on the surface of the cell is not easy to have contact with infection protein of the virus, or the oxetanocin has reaction with the receptor on the surface of the cell so that the receptor is not easy to have contact with other proteins.

Description

technical field [0001] The invention belongs to the field of western medicine and relates to the application of thetasu in the preparation of antiviral drugs. Background technique [0002] Influenza virus, referred to as influenza virus, is an RNA virus that causes influenza in humans and animals. In taxonomy, influenza virus belongs to the Orthomyxoviridae family. It can cause acute upper respiratory tract infections and rapidly spread through the air. There are often periodic pandemics around the world. Influenza viruses include human influenza viruses and animal influenza viruses. Human influenza viruses are divided into three types: A (A), B (B), and C (C), which are the pathogens of influenza (flu). Among them, the antigenicity of influenza A virus is easy to mutate, causing worldwide pandemics many times. For example, in the pandemic from 1918 to 1919, at least 20 million to 40 million people died of influenza in the world; influenza B virus was less pathogenic to hu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61P31/12A61P31/16A61P31/18
Inventor 鄢新民刘娟鄢紫雯庞慎吴克宁
Owner 江西蓝十字生物科技有限公司
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