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Rapid transfer breeding method for Shanxi 5201 sterile line

A sterile line and rapid technology, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve problems such as limiting the application range of sterile sources, and achieve the effect of improving seed production and reducing costs.

Inactive Publication Date: 2017-09-22
HENAN XINXIANG ACADEMY OF AGRI SCI
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AI Technical Summary

Problems solved by technology

The Chinese cabbage nuclear gene male sterile line with 100% sterile plant rate, its maintainer line can only temporarily maintain the sterility of the sterile plant for one generation. The scope of application of the sterile source

Method used

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  • Rapid transfer breeding method for Shanxi 5201 sterile line
  • Rapid transfer breeding method for Shanxi 5201 sterile line

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Experimental program
Comparison scheme
Effect test

Embodiment

[0012] 1. Transformation of Shan 5201 Chinese cabbage inbred line with three sterile sources

[0013] The source of the three sources of sterility and the mechanism and performance of sterility:

[0014] (1) Chinese cabbage sterility source: it belongs to the source of cytoplasmic male sterility of Chinese cabbage Brassica napus. Temperature-sensitive type, fertile at low temperature, sterile at high temperature; there is a phenomenon of dead buds.

[0015] (2) Vegetable sprout sterility source: it is the nucleus-cytoplasmic interaction sterility source discovered and purified by the applicant from the original variety of vegetable sprout. There are two kinds of restorer lines and maintainer lines that can be produced by inbred lines of Chinese cabbage. When the material is fully purified, the maintainer line can obtain 100% sterility rate and 100% sterility degree, and the phenomenon of dead buds is relatively light.

[0016] (3) The source of sterility of Ziqinggengcai: I...

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Abstract

The invention discloses a method for rapid breeding of the sterile line of Shaanxi 5201. The breeding method includes the following steps: the implantation effect of the self-incompatible line 5201 is the best, and can achieve 100% infertility. Fertility rate and 100% sterility; in the process of male sterile line transfer of Shaanxi 5201, by selecting a single plant similar to the maintainer line and with normal flower bud development from the second-generation segregation population to continue the transfer, it can be quickly obtained Purpose of transfer. At the same time, selecting a single plant with a high affinity index for sterile line transfer will greatly reduce the cost of breeding parents and increase the yield of seed production.

Description

technical field [0001] The invention relates to the field of agricultural breeding, in particular to a method for rapid breeding of a male sterile line of Shaanxi 5201. Background technique [0002] The male sterile dual-purpose line (single-gene recessive male sterility) discovered and pioneered by Chinese scholars in the early 1970s was once bred into varieties and used in production (such as Chinese cabbage 156, Shenyang Kuaicai, Zhengzhou Zaohei leaves, etc.), but because 50% of the fertile plants need to be pulled out many times during seed production, it is time-consuming and labor-intensive, and the yield of seed production is often low, thereby limiting the development and utilization of this sterile type. Zhang Shufang of Shenyang Academy of Agricultural Sciences bred for the first time the nuclear gene interaction male sterile line and bred the hybrid "Kuaicai No. 1" and other large-scale popularization and application. This kind of male sterile line, the sterilit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 原连庄原让花肖艳王晓玲原静云杜亮吴涛
Owner HENAN XINXIANG ACADEMY OF AGRI SCI
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