Seedling breeding method of Rhododendron fortunei

A rhododendron and bud seedling technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of not being suitable for large-scale seedling cultivation, long time, cost waste, etc., and achieve rich nutrition, fast breeding speed, and flowers lush effect

Inactive Publication Date: 2017-04-26
蚌埠市金牛湾农业科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current propagation methods mainly include sowing seedlings and cutting seedlings. The former takes a long time to germinate seeds, and the germination rate is extremely low, which is not suitable for larg

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for raising seedlings of Rhododendron yunnanensis, comprising the following steps:

[0020] (1) Primary generation induction culture

[0021] Select high-quality rhododendron stems as explants, cut off both ends after sterilization, leave the middle 1-2 cm long, inoculate into callus induction medium for primary induction culture, and cultivate for 30 days to obtain clustered buds , the callus induction medium consists of the following components in parts by weight: 70 parts of White medium, 10 parts of potato juice, 7 parts of kinetin, 5 parts of acid hydrolyzed casein, 4 parts of phosphatidylinositol, glutamine 3 parts, 2 parts of fumaric acid, 0.3 parts of viola extract, 0.2 parts of saxifrage extract, and 0.1 part of Agrimony extract. The culture conditions are: light intensity 1500Lx, photoperiod 9.5h / d, temperature 23°C, air humidity 65%, pH 6.3;

[0022] (2) Cluster bud proliferation culture

[0023] The clustered buds that step (1) is cultivated are ...

Embodiment 2

[0031] A method for raising seedlings of Rhododendron yunnanensis, comprising the following steps:

[0032] (1) Primary generation induction culture

[0033] Select high-quality rhododendron stems as explants, cut off both ends after sterilization, leave the middle 1-2 cm long, inoculate into callus induction medium for primary induction culture, and cultivate for 32 days to obtain clustered buds , the callus induction medium consists of the following components in parts by weight: 72 parts of White medium, 11 parts of potato juice, 8 parts of kinetin, 6 parts of acid hydrolyzed casein, 5 parts of phosphatidylinositol, glutamine 4 parts, 3 parts of fumaric acid, 0.4 parts of viola extract, 0.3 parts of saxifrage extract, and 0.15 parts of Agrimony extract. The culture conditions are: light intensity 1700Lx, photoperiod 9.5h / d, temperature 24°C, air humidity 65%, pH 6.3;

[0034] (2) Cluster bud proliferation culture

[0035] The clustered buds that step (1) is cultivated ar...

Embodiment 3

[0043] A method for raising seedlings of Rhododendron yunnanensis, comprising the following steps:

[0044] (1) Primary generation induction culture

[0045] Select high-quality rhododendron stems as explants, cut off both ends after sterilization, leave the middle 1-2 cm long, inoculate into callus induction medium for primary induction culture, and cultivate for 35 days to obtain clustered buds , the callus induction medium consists of the following components in parts by weight: 75 parts of White medium, 12 parts of potato juice, 9 parts of kinetin, 8 parts of acid hydrolyzed casein, 6 parts of phosphatidylinositol, glutamine 5 parts, 4 parts of fumaric acid, 0.5 parts of viola extract, 0.4 parts of saxifrage extract, and 0.2 parts of Agrimony extract. The culture conditions are: light intensity 2000Lx, photoperiod 9.5h / d, temperature 25℃, air humidity 65%, pH 6.3;

[0046] (2) Cluster bud proliferation culture

[0047] The clustered buds that step (1) is cultivated are ...

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Abstract

The invention relates to the field of flower planting, in particular to a seedling breeding method of Rhododendron fortunei. According to the method, seedlings of the Rhododendron fortune are bred with a tissue culture technique, so that the breeding speed is high, obtained seedlings are improved varieties, the plant shape is attractive, leaf color is invisible green and flowers are flourishing after the seedlings grow up, the flowering stage is 15-20 days longer than that of existing varieties, and ornamental value is improved; due to addition of Chinese herbal medicine extract to a culture medium, the disease resistance of Rhododendron fortunei plants is enhanced, and the occurrence rate of pest and disease damage of the Rhododendron fortunei is reduced.

Description

technical field [0001] The invention relates to the field of flower planting, in particular to a method for raising seedlings of rhododendron versicolor. Background technique [0002] Rhododendron yunjin is an evergreen shrub or small tree, 3-12 meters high; the main trunk is curved, the bark is brown and cracked in flakes; the young branches are yellow-green, with glands beginning to form; the old branches are gray-brown. Thick leathery leaves, oblong to oblong-elliptic, dark green and shiny above, light green below. Terminal raceme umbel loose, with 6-12 flowers, fragrant; corolla funnel-shaped bell-shaped, 4.5-5.2 cm long, 5-5.5 cm in diameter, pink, with sparse glands outside, 7 lobes, broad oval. Capsule oblong-ovate to oblong-elliptic, straight or slightly curved. The flowering period is April-May, and the fruiting period is August-October. Born in the sun or under forests on mountain ridges at an altitude of 620-2000 meters. Produced in Shaanxi, Hubei, Hunan, Hen...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 余安芹苏丹青
Owner 蚌埠市金牛湾农业科技发展有限公司
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