A kind of allium plant bulb tissue culture method

A technology for allium plants and tissue culture, applied in the field of plant tissue culture, can solve the problems of low induction efficiency and low proliferation rate of allium plants, and achieves a method of accelerating callus and culture growth, high proliferation rate and rich nutrition. Effect

Inactive Publication Date: 2019-03-08
NANJING XIAOZHUANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, the technical problem to be solved in the present invention is to overcome the low induction efficiency and low multiplication rate defects of using plant tissue culture technology to cultivate Allium plants in the prior art, thereby providing a high-efficiency Allium plant bulb tissue culture method

Method used

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  • A kind of allium plant bulb tissue culture method
  • A kind of allium plant bulb tissue culture method
  • A kind of allium plant bulb tissue culture method

Examples

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preparation example Construction

[0025] The preparation method of MS culture medium used in the present invention is as follows:

[0026] 1) Mother liquor configuration

[0027] Refer to Tables 1-4 and weigh each component separately.

[0028] Table 1 MS macroelement mother liquor formula

[0029]

[0030] After weighing, dissolve them with a small amount of distilled water and mix them, and finally make up to 1L.

[0031] Table 2 MS trace element mother liquor formula

[0032]

[0033] After weighing, dissolve them with a small amount of distilled water and mix them, and finally make up to 1L.

[0034] Table 3 MS inositol mother liquor formula

[0035]

[0036] After weighing, dissolve them with a small amount of distilled water and mix them, and finally make up to 1L.

[0037] Table 4 MS iron salt mother liquor formula

[0038]

[0039] After weighing, dissolve them with a small amount of distilled water and mix them, and finally make up to 1L.

[0040] 2) MS medium configuration

[0041...

Embodiment 1

[0058] The present embodiment provides a method for onion bulb tissue culture, comprising the following steps:

[0059] (1) Select the underground bulb of green onion as the explant, after removing the outer two layers of bulbs, rinse with tap water for 15 minutes, rinse with alcohol with a volume percentage concentration of 75% for 15 minutes, and then soak in HgCl with a mass fraction of 0.1% 2 After sterilizing in the solution for 10min, after taking it out, rinse it with sterile water for 5 times for 30s each time, and then remove the surface water with sterile paper;

[0060] (2) Inoculate the sterilized explants in MS medium with pH 6.2, the MS medium used contains 2,4-dichlorophenoxyacetic acid with a concentration of 0.3 mg / L and a concentration of 0.5 mg / L Thidiazuron and vitamin B at a concentration of 1.0mg / L 6 , and 3% sucrose, cultured at 35°C for 4 days in the dark, then at 25°C for 15 days in the dark, then under the condition of 1300Lx light intensity and 25°C...

Embodiment 2

[0064] The present embodiment provides a leek bulb tissue culture method, comprising the following steps:

[0065] (1) Select the underground bulb of leek as the explant, after removing the outer layer of bulbs, rinse with tap water for 10 minutes, rinse with alcohol with a concentration of 70% by volume for 20 minutes, and then soak in HgCl with a mass fraction of 0.1% 2 After sterilizing in the solution for 8 minutes, take it out and rinse it with sterile water 4 times for 40s each time, and then remove the surface water with sterile paper;

[0066] (2) Inoculate the sterilized explants in MS medium with pH 6.5, the MS medium used contains 2,4-dichlorophenoxyacetic acid with a concentration of 0.5 mg / L and a concentration of 0.1 mg / L thidiazuron and vitamin B at a concentration of 1.5mg / L 6 , and 2% sucrose, cultured at 30°C for 5 days in the dark, then at 28°C for 10 days in the dark, and then under the condition of 1500Lx light intensity and 22°C, light for 14 hours a day...

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Abstract

The invention discloses a method for cultivating bulb tissues of alliums. The method comprises the following steps: selecting the underground bulbs of the alliums as explants and performing disinfection treatment; inoculating the explants to an MS culture medium containing 2,4-dichlorphenoxyacetic acid with the concentration of 0.1 to 0.5 mg / L, thidiazuron with the concentration of 0.1 to 1.0 mg / Land vitamin B6 with the concentration of 0.5 to 1.5 mg / L, and sequentially performing heat shock treatment, lucifugal cultivation and illumination cultivation; and transplanting the explants to a White culture medium containing indolebutyric acid with the concentration of 0.1 to 0.5 mg / L, thidiazuron with the concentration of 0.1 to 1.0 mg / L and vitamin B1 with the concentration of 8 to 10 mg / L and performing rooting cultivation. Different culture mediums are used according to the nutritional requirements in different cultivation stages, the corresponding cultivation method is assisted, and synergistic effect is achieved, so that the adventitious bud induction efficiency and rooting rate are effectively improved, the proliferation rate is increased, the proliferation time is short and thebreeding speed is high.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to a method for culturing bulb tissue of an Allium plant. Background technique [0002] Allium plants are important vegetables and condiments in my country, and are also widely used in Japan, South Korea and Southeast Asian countries. The traditional way of reproduction is mostly by balls or seeds, but the production of balls or seeds takes 3-4 years, sowing production is limited by season or climate, and the number of reproduction is limited, and allium plants are mostly cross-pollinated crops, with multiple generations. Selfing decline is serious, can not guarantee the quality of reproduction. In addition, some plants of the Allium genus have been underground for a long time and are susceptible to virus infection. Once the bulbs or seeds are infected with the virus, they cannot be eradicated, resulting in a substantial decline in the quality of the progeny. [0003]...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 杨平张边江王立科唐宁陈全战
Owner NANJING XIAOZHUANG UNIV
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