A kind of allium plant bulb tissue culture method
A technology for allium plants and tissue culture, applied in the field of plant tissue culture, can solve the problems of low induction efficiency and low proliferation rate of allium plants, and achieves a method of accelerating callus and culture growth, high proliferation rate and rich nutrition. Effect
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[0025] The preparation method of MS culture medium used in the present invention is as follows:
[0026] 1) Mother liquor configuration
[0027] Refer to Tables 1-4 and weigh each component separately.
[0028] Table 1 MS macroelement mother liquor formula
[0029]
[0030] After weighing, dissolve them with a small amount of distilled water and mix them, and finally make up to 1L.
[0031] Table 2 MS trace element mother liquor formula
[0032]
[0033] After weighing, dissolve them with a small amount of distilled water and mix them, and finally make up to 1L.
[0034] Table 3 MS inositol mother liquor formula
[0035]
[0036] After weighing, dissolve them with a small amount of distilled water and mix them, and finally make up to 1L.
[0037] Table 4 MS iron salt mother liquor formula
[0038]
[0039] After weighing, dissolve them with a small amount of distilled water and mix them, and finally make up to 1L.
[0040] 2) MS medium configuration
[0041...
Embodiment 1
[0058] The present embodiment provides a method for onion bulb tissue culture, comprising the following steps:
[0059] (1) Select the underground bulb of green onion as the explant, after removing the outer two layers of bulbs, rinse with tap water for 15 minutes, rinse with alcohol with a volume percentage concentration of 75% for 15 minutes, and then soak in HgCl with a mass fraction of 0.1% 2 After sterilizing in the solution for 10min, after taking it out, rinse it with sterile water for 5 times for 30s each time, and then remove the surface water with sterile paper;
[0060] (2) Inoculate the sterilized explants in MS medium with pH 6.2, the MS medium used contains 2,4-dichlorophenoxyacetic acid with a concentration of 0.3 mg / L and a concentration of 0.5 mg / L Thidiazuron and vitamin B at a concentration of 1.0mg / L 6 , and 3% sucrose, cultured at 35°C for 4 days in the dark, then at 25°C for 15 days in the dark, then under the condition of 1300Lx light intensity and 25°C...
Embodiment 2
[0064] The present embodiment provides a leek bulb tissue culture method, comprising the following steps:
[0065] (1) Select the underground bulb of leek as the explant, after removing the outer layer of bulbs, rinse with tap water for 10 minutes, rinse with alcohol with a concentration of 70% by volume for 20 minutes, and then soak in HgCl with a mass fraction of 0.1% 2 After sterilizing in the solution for 8 minutes, take it out and rinse it with sterile water 4 times for 40s each time, and then remove the surface water with sterile paper;
[0066] (2) Inoculate the sterilized explants in MS medium with pH 6.5, the MS medium used contains 2,4-dichlorophenoxyacetic acid with a concentration of 0.5 mg / L and a concentration of 0.1 mg / L thidiazuron and vitamin B at a concentration of 1.5mg / L 6 , and 2% sucrose, cultured at 30°C for 5 days in the dark, then at 28°C for 10 days in the dark, and then under the condition of 1500Lx light intensity and 22°C, light for 14 hours a day...
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