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Treating method for chemically inducing parthenogenesis of corn

A technology of parthenogenesis and treatment method, which is applied in the field of corn breeding, can solve the problems of pollen pollution, achieve the effect of prolonging the residence time, increasing the induction rate, and simple and easy operation

Active Publication Date: 2015-05-13
SHENYANG TEYIJIA CORN TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0019] The technical problems to be solved by the present invention: one is to completely solve the problem of pollen pollution produced in a large amount in the induction treatment operation, and no false seeds will be produced; Third, it can avoid a large amount of liquid medicine flowing caused by smearing or injection, and the waste of liquid medicine caused by spraying, which reduces the cost; fourth, it simplifies the processing procedure, improves the induction rate, and reduces the amount of inducer , making the operation simple and easy for large-scale application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] (1) Preparation of inducer

[0049] Select trifluralin with a concentration of 48% sold on the market and dilute it with water to form an inducer with a concentration of 80 μmol / L.

[0050] (2) Prepare paper paste

[0051] Use the roll paper for daily use sold in the market, add an appropriate amount of roll paper and the inducer liquid into the container, and stir it into a thin paper paste, the thickness of which can be grasped with 20cm dressing tweezers.

[0052] (3) Select processing materials

[0053] The object of treatment is the maize hybrid "Liaodan 565".

[0054] (4) Handle fruit ears

[0055]Bagging the ear before silking; when the filaments are completely spun out, remove the paper bag, and peel off the outer multi-layered bracts at the top of the ear buds and above the young ears, leaving only the filaments and the ones close to the filaments The inner layer of bracts, then use a blade to cut off the unopened part horizontally at the base to form a cir...

Embodiment 2

[0063] (1) Preparation of inducer

[0064] Trifluralin sold in the market was selected, and 48% trifluralin was diluted with water to form an inducer with a concentration of 90 μmol / L.

[0065] (2) Prepare paper paste

[0066] Use the daily roll paper sold in the market, put the roll paper and the inducer liquid into the container, and stir it into a thin paper paste.

[0067] (3) Select processing materials

[0068] The object of treatment is waxy corn hybrid "Shen Nuo 3".

[0069] (4) Handle fruit ears

[0070] Bagging the ear before silking; when most of the filaments are completely spun out, remove the paper bag, and peel off the outer multi-layered bracts at the top of the ear buds and above the young ears, leaving only the filaments and close The inner bracts of the filaments are cut off laterally at the base with a blade, forming a section with bracts around the filaments in the middle.

[0071] (5) Apply inducer

[0072] Use 20cm dressing tweezers to pick up the ...

Embodiment 3

[0078] (1) Preparation of inducer

[0079] The wettable powder containing 50% benzamide sold on the market was selected and diluted with water to obtain an inducer with a concentration of 40 μmol / L.

[0080] (2) Prepare paper paste

[0081] Use the roll paper for daily use sold in the market, put the roll paper and the inducer solution into the container, and stir it into a thin paper paste.

[0082] (3) Select processing materials

[0083] The object of treatment is popcorn hybrid "Shenbo No. 3".

[0084] (4) Handle fruit ears

[0085] Bagging the ear before silking; when most of the filaments are completely spun out, remove the paper bag, and peel off the outer multi-layered bracts at the top of the ear buds and above the young ears, leaving only the filaments and close The inner bracts of the filaments are cut off laterally at the base with a blade, forming a circular section with bracts around the filaments in the middle.

[0086] (5) Apply inducer

[0087] Use tweez...

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Abstract

The invention provides a treating method for chemically inducing parthenogenesis of corn. The method is characterized by comprising the following steps: adding roll paper into an inductive agent and carrying out stirring so as to prepare paper paste; sleeving a female ear with a paper bag before filament emergence; picking off the paper bag when filaments come out and transversely cutting off a basal part at the top of the bud of the female ear with a blade so as to form a circular cross section which has filaments at the center and husk leaves at the periphery; picking up the paper paste with dressing pliers, placing the paper paste on the cross section and allowing the paper paste to closely contact with the cross section and fill in space formed after the cutting; restoring pulled-open husk leaves to original positions so as to allow the paper paste to be packed in the husk leaves; and finally, sleeving the treated ear with the paper bag again. The invention has the following beneficial effects: the problem of pollen pollution difficult to overcome in a conventional chemical-induction treatment method is thoroughly overcome; threat to human health is avoided; induction operation is carried out under completely open conditions; an induction rate is improved; loss of the liquid of the inductive agent is small; and compared with a conventional selfing line breeding method, the breeding period in the method provided by the invention is shortened by more than 5 generations.

Description

technical field [0001] The invention belongs to the technical field of corn breeding, in particular to a method for breeding corn inbred lines. Background technique [0002] In the conventional method of breeding maize inbred lines, breeding an inbred line requires at least 7 consecutive generations of selfing to achieve genotype homozygosity, and more generations are required for materials with complex genetic backgrounds. In order to speed up the breeding of inbred lines, shorten the breeding cycle and improve the purity, the methods commonly used at home and abroad mainly include the Stock 6 genetically induced haploid method and the chemically induced parthenogenesis method: [0003] 1.Stock 6 induction method [0004] The induced subjects were pollinated with genetic induction line Stock 6, and maize was induced to produce haploid seeds. Then use colchicine and other chromosome doubling agents to double them into diploid plants. This method is considered to be the mo...

Claims

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Application Information

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IPC IPC(8): A01H1/08
Inventor 史振声张喜华
Owner SHENYANG TEYIJIA CORN TECH
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