Breeding method of new high-yield lodging-resistant disease-resistant wheat variety
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A new variety of wheat technology, applied in botany equipment and methods, biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems affecting high yield, loose plant type, easy lodging, etc.
Active Publication Date: 2021-07-30
JIANGSU LIXIAHE REGION AGRI RES INST
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[0004] The biggest disadvantage of Yangmai 18 is that the stalk has poor elasticity or toughness, the plant type is loose, and it is easy to lodging, which affects its high yield.
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Embodiment 1
[0027] Embodiment 1 A kind of breeding method of high-yield, lodging-resistant and disease-resistant wheat new varieties
[0028] 1) At the beginning of January 2015, Yuanyou-2, an intermediate wheat material with short stalks and large panicles and strong toughness against lodging, and Yangmai 18, a wheat variety resistant to scab, were planted in the greenhouse. In April, Yuanyou-2 was used as the female parent. Yangmai 18 was used as the male parent, and a hybrid combination was prepared, and a hybrid F1 was obtained at the end of May;
[0029] The intermediate material of dwarf, large spike and lodging resistant wheat is Yuanyou-2, which is an intermediate material with dwarf, large spike and hard stalk provided by the research group of Mr. Li Lihui of the Chinese Academy of Agricultural Sciences during the implementation of the 973 project. It is planted in the Institute of Crop Science, Chinese Academy of Agricultural Sciences, and the national uniform number is ZM017976...
Embodiment 2
[0039] Example 2: Molecular marker-assisted selection of the QGns-YY-5B / QGbs-YY-5B genotype method for the number of grains per ear and the number of grains at the base of the panicle synergistic site:
[0040] The materials of step 5 and step 6 in embodiment 1 were detected respectively with molecular marker Xgpw4483 primer set;
[0041] 1. Use the CTAB method (reference: Stacey J, Isaac PG. Isolation of DNA from plants. Methods Mol. Biol. 1994, 28:9–15) to extract the F3 single plant in step 5, and the F4 plant in the seedling stage of step 6 The mixed leaf genomic DNA was diluted to obtain a template solution with a DNA concentration of about 30 ng / μL.
[0042] 2. According to the existing references (Hu Wenjing, Gao Derong, Lu Chengbin, etc., QTL mapping of wheat panicle traits and plant height and analysis of T6VS.6AL translocation effect, Journal of Wheat Crops, 2019, 39(5): 505- 512), the number of grains per panicle QGns-YY-5B and the number of grains per panicle base...
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Abstract
The invention discloses a breeding method of a new high-yield lodging-resistant disease-resistant wheat variety. The method comprises the following steps: hybridizing and backcrossing a dwarf, large-ear and hard-stalk lodging-resistant wheat intermediate material serving as a female parent and a disease-resistant variety Yangmai 18 serving as a male parent for one time, adding generations in a greenhouse, selecting single plants for inducing powdery mildew and resisting powdery mildew in the generation adding process, then performing molecular marker selection by combining synergistic sites QGns-YY-5B/QGbs-YY-5B of the number of grains per spike and the number of fruiting grains per spike base, planting selected strains into plant rows in a field, performing artificial induction selection on powdery mildew and gibberellic disease of advanced generations, and selecting comprehensive agronomic traits, stalk toughness lodging resistance and disease resistance. The new wheat variety bred by the method disclosed by the invention stably inherits the gibberellic disease and powdery mildew resistance of Yangmai 18, the excellent characters of good stem toughness and good lodging resistance of a non-recurrent parent are added, and the average yield is remarkably higher than that of the Yangmai 18.
Description
technical field [0001] The invention belongs to the technical field of crop breeding methods, and relates to a breeding method for a high-yield, lodging-resistant, and disease-resistant new wheat variety. Background technique [0002] The rice stubble area in the middle and lower reaches of the Yangtze River in my country is a frequent and recurrent area of wheat scab. On the one hand, the climatic conditions, including temperature and humidity, during the wheat flowering period (April to May) in this area are most suitable for the onset of scab. On the other hand, the head blight fungus will survive summer and winter on the rice piles, resulting in long-term source of head blight infection in the planting area. From 2012 to 2015, the average annual occurrence area of scab in Jiangsu Province was about 1.2 million hm 2 . The infection period of head blight is mainly during the flowering stage of wheat. After the head blight infects the florets, it rapidly expands in the...
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