Breeding method of new high-yield lodging-resistant disease-resistant wheat variety
A new variety of wheat technology, applied in botany equipment and methods, biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems affecting high yield, loose plant type, easy lodging, etc.
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Embodiment 1
[0027] Embodiment 1 A kind of breeding method of high-yield, lodging-resistant and disease-resistant wheat new varieties
[0028] 1) At the beginning of January 2015, Yuanyou-2, an intermediate wheat material with short stalks and large panicles and strong toughness against lodging, and Yangmai 18, a wheat variety resistant to scab, were planted in the greenhouse. In April, Yuanyou-2 was used as the female parent. Yangmai 18 was used as the male parent, and a hybrid combination was prepared, and a hybrid F1 was obtained at the end of May;
[0029] The intermediate material of dwarf, large spike and lodging resistant wheat is Yuanyou-2, which is an intermediate material with dwarf, large spike and hard stalk provided by the research group of Mr. Li Lihui of the Chinese Academy of Agricultural Sciences during the implementation of the 973 project. It is planted in the Institute of Crop Science, Chinese Academy of Agricultural Sciences, and the national uniform number is ZM017976...
Embodiment 2
[0039] Example 2: Molecular marker-assisted selection of the QGns-YY-5B / QGbs-YY-5B genotype method for the number of grains per ear and the number of grains at the base of the panicle synergistic site:
[0040] The materials of step 5 and step 6 in embodiment 1 were detected respectively with molecular marker Xgpw4483 primer set;
[0041] 1. Use the CTAB method (reference: Stacey J, Isaac PG. Isolation of DNA from plants. Methods Mol. Biol. 1994, 28:9–15) to extract the F3 single plant in step 5, and the F4 plant in the seedling stage of step 6 The mixed leaf genomic DNA was diluted to obtain a template solution with a DNA concentration of about 30 ng / μL.
[0042] 2. According to the existing references (Hu Wenjing, Gao Derong, Lu Chengbin, etc., QTL mapping of wheat panicle traits and plant height and analysis of T6VS.6AL translocation effect, Journal of Wheat Crops, 2019, 39(5): 505- 512), the number of grains per panicle QGns-YY-5B and the number of grains per panicle base...
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