Method used for quickly breaking dormancy of peony seed epicotyl

A peony seed, high-efficiency technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as unsuccessful reports, achieve high phenotypic consistency, shorten the process of cultivation and genetic improvement, and improve efficiency Effect

Inactive Publication Date: 2014-06-11
SHANGHAI HIGH SCHOOL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] According to domestic and foreign literature inquiries, the method of breaking the epicotyl dormancy of peony in this study is unique, the formula is unique, the speed is fast, and the effect is good. So far, there has been no successful report.

Method used

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  • Method used for quickly breaking dormancy of peony seed epicotyl
  • Method used for quickly breaking dormancy of peony seed epicotyl

Examples

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Embodiment 1

[0026] Epicotyl dormancy breaking method for soil germinated peony seeds:

[0027] (1) Select plump peony seeds, soak them in treatment solution I for 24 hours, discard the solution, keep the seeds, wash with warm water 3 times, air-dry, and put them in a 4°C refrigerator for later use;

[0028] (2) Take some clean river sand, sterilize at 121°C for 1 hour, cool to room temperature, soak the river sand with treatment solution II, bury the peony seeds treated in step (1) in the above wet sand, and keep it under dark conditions for 4 Stored at ℃ for 1 month, the roots appear and germination begins;

[0029] (3) Shallowly sow the rooted peony seeds in step (2) in a substrate with a mass ratio of peat, vegetable garden soil, and perlite at a ratio of 3:6:1, and grow one in a greenhouse at 28±2°C under natural light. month, petioles and leaves appear;

[0030] (4) Spray the leaves of the seedlings obtained in step (3) with the treatment liquid III, 7 days / time, and continue spray...

Embodiment 2

[0033] Epicotyl dormancy breaking method for culturing peony seed embryos in vitro:

[0034] (1) Select plump peony seeds, soak them in treatment solution I for 24 hours, discard the solution, keep the seeds, wash with warm water 3 times, air-dry, and put them in a refrigerator at 4°C or -20°C for later use;

[0035] (2) Peel off the seed shells of the peony seeds obtained in step (1), soak them in the treatment solution II for 24 hours, discard the solution, collect the seeds with endosperm, and absorb the surface moisture with filter paper;

[0036] (3) Wash the seeds with endosperm obtained in step (2) with tap water, blot the surface water droplets, disinfect with 70% volume concentration of ethanol for 30-90 seconds on the ultra-clean workbench, soak in 0.1wt% mercury chloride After 5-8 minutes, rinse with sterile water for 5-8 times, dry the water drops on the surface of the sterile material with sterile filter paper, peel off the seed embryos, and inoculate them in WPM ...

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Abstract

The invention discloses a method used for quickly breaking dormancy of a peony seed epicotyl. With the method, the dormancy of the peony seed epicotyl supposed to naturally germinate in two years can be quickly broken, and the peony seed can germinate smoothly after being processed for three months; under the isolated culture condition, the dormancy characteristic of the peony seed epicotyl can be broken after the processing period of two weeks. With the adoption of the two methods disclosed by the invention, high-quality peony seedlings can be obtained; the peony nursery stock germinating quickly after processing is highly consistent with the phenotype of peony seedlings obtained by the traditional method, in other words, the parental genetic phenotype is maintained, and the seed germination, seedling and flow formation processes are greatly shortened; besides, the method can be used for creating heterogeneous parental new germplasm, so that the efficiency of traditional breeding and cross breeding of peony is improved, and the breeding and genetic improvement processes of peony are shortened.

Description

technical field [0001] The invention relates to a method for rapidly and efficiently breaking the epicotyl dormancy of peony seeds. Background technique [0002] Peony (Paeonia suffruticosa), also known as rich flower, hundred taels of gold, wood peony, rat aunt, deer leek, Luoyang flower, is a woody plant of Paeoniaceae Paeoniae, a deciduous shrub, known as "national beauty and heavenly fragrance", "the best flower among flowers". The reputation of "King" is the first choice for my country's national flower. Peony not only has high ornamental value, but also has good medicinal value. As early as the Han Dynasty, there was a record of peonies being domesticated and used as medicine in the ancient book of medicinal plants "Shen Nong's Materia Medica". With the increase of the market value of peony in landscaping and medicinal material storage, the economic market of peony has been born. Expanding the reproduction coefficient, improving the propagation efficiency, carrying o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G7/06A01H4/00
Inventor 张逸伦
Owner SHANGHAI HIGH SCHOOL
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