Molecular marker of high erucic acid gene in Brassica napus L. and breeding method thereof

Abstract

The invention discloses a molecular marker of a high erucic acid gene in Brassica napus L. and a breeding method thereof and relates to the biological breeding field. The method comprises the following steps of: S1, constructing a segregating population; 2, extract that genomic DNA of the material to be tested by the CTAB method; 3, carry out PCR amplification; S4, using marker primer pairs Pb1, Pb2, Fa1, Fa2 to detect whether a single plant in the segregated population contains root swelling resistance site PbBa8.1 or low erucic acid gene fae1 and classify; S5, a new rapeseed variety with high erucic acid and root swelling resistance locus PbBa 8.1 and low erucic acid and low erucic acid and low erucic acid and low erucic acid and low erucic acid and low erucic acid and low erucic acid and low erucic acid and low erucic acid and low erucic acid and low erucic acid and low erucic acid and high erucic acid and low erucic acid were selected. The method for selecting and breeding rapeseedwith root swelling disease resistance and low erucic acid in grain can be used for auxiliary selection of root swelling disease resistance site PbBa8.1 and low erucic acid gene fae1 with genetic linkage at seedling stage, The theoretical accuracy of gene-specific markers reached 100%, which improved the breeding efficiency of rapeseed resistant to root swelling and low erucic acid, and shortenedthe breeding process.

Description

technical field [0001] The invention relates to the technical field of breeding, in particular to a molecular marker and a breeding method for a high erucic acid gene of Brassica napus. Background technique [0002] Cruciferous clubroot is a worldwide disease caused by the infection of Plasmodiophora brassicae Woron. It has spread rapidly in rapeseed areas in my country in recent years. With the continuous improvement of the level of agricultural mechanization, clubroot will spread more rapidly, seriously threatening the safety of my country's rapeseed industry. After the host is infected by Plasmodium fungus, small nodules first appear on the lateral roots, and as the disease progresses, enlarged white tissue will appear in the main root. The main root of severely infected plants may be completely destroyed, resulting in damage to xylem cells and interruption of water transport, thereby Causes stunting and a series of above-ground symptoms such as wilting, yellowing, antho...

AI Technical Summary

Problems solved by technology

[0007] The physical distance between the A08-300 marker and the PbBa8.1 site is 20.7kb and the linkage is tight. In the case of a certain size of population, clubroot resistant materials can be effectively bred, but theoretically, the marker and PbBa8.1 may still occur. Swap, causing false positives when the population is large enough

During the breeding process, while the anti-clubber locus PbBa8.1 is being transgenic, to remove the homoerucic acid linked to it generally requires planting a larger segregation population, which will take up more space and land, plus natural inoculation or Manual inoculation identification requires a large workload and low accuracy; and the erucic acid trait needs to be identified after harvesting the seeds, which greatly reduces the efficiency of disease-resistant breeding

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