Resistance identification method of sweet potato scab for stem tip vegetables
A resistance identification and scab technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of increasing the risk of pesticide residues and environmental pollution, increasing labor costs, etc., to achieve the goal of increasing pesticide residues and environmental protection Risk of contamination, increased labor costs, effects of stable results
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Embodiment 1
[0061] The invention provides a method for identifying resistance to sweet potato scab for stem apex vegetables, comprising the following steps:
[0062] S1. Preparation of sweet potato materials to be identified: Select 10 sweet potato varieties or strains for stem tip vegetables such as Fucai 13-14, Guangcaishu No. 7 and Fucaishu 25, and plant them in Zhongluotan Baiyun Base of Guangdong Academy of Agricultural Sciences. According to the observation of the traits, the growth is healthy and disease-free, and it will be used after multiplication.
[0063] S2. Preparation and activation of pathogenic bacteria: activate the sweet potato scab strain CJ1 stored in the Crop Research Institute of Guangdong Academy of Agricultural Sciences, inoculate it on a PDA medium plate, and cultivate it under dark conditions at 25°C. After the mycelium grows on the PDA medium plate , for inoculum preparation.
[0064] S3. Preparation of inoculum: S2 activated pathogenic bacteria were propagate...
Embodiment 2
[0087] Utilize the method of the present invention to carry out disease-resistant identification to 28 parts of stem tip vegetable sweet potato varieties or strain materials, comprise the following steps:
[0088] S1. Preparation of sweet potato materials to be identified: Select 28 varieties or strains of sweet potato varieties or strains for stem tip vegetables, including Guangcaishu 11-52, Qiancaishu No. 2 and Fucaishu 18, and plant them in Zhongluotan Baiyun Base, Guangdong Academy of Agricultural Sciences , through the observation of traits, the growth is healthy and disease-free, and it will be used after multiplication.
[0089] S2. Preparation and activation of pathogenic bacteria: activate the sweet potato scab strain CJ1 stored in the Crop Research Institute of Guangdong Academy of Agricultural Sciences, inoculate it on a PDA medium plate, and cultivate it under dark conditions at 25°C. After the mycelium grows on the PDA medium plate , for inoculum preparation.
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