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Method for protecting and restoring germplasm of citrus-Hunglongbing-infected citrus according to two-step method

A technology for huanglongbing and citrus, which is applied in the field of two-step conservation of citrus germplasm infected with huanglongbing, can solve problems such as unseen, achieve better effect, less damage, and reduce the probability of drug resistance

Inactive Publication Date: 2016-10-26
PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After searching, there is no use of enrofloxacin sodium and sulfadimethoxine sodium fungicide composition and heat treatment-fungicide two-step method to protect the fine citrus species that are endangered due to the serious harm of Huanglongbing quality

Method used

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  • Method for protecting and restoring germplasm of citrus-Hunglongbing-infected citrus according to two-step method
  • Method for protecting and restoring germplasm of citrus-Hunglongbing-infected citrus according to two-step method
  • Method for protecting and restoring germplasm of citrus-Hunglongbing-infected citrus according to two-step method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Firstly, the content of Huanglongbing bacteria in the infected mother tree was measured, and the data of month 0 were marked.

[0026] Then it is detoxified, and all steps are carried out in a 100-mesh insect-free net room. The specific steps are as follows: take the seeds of the mother tree and immerse them in 70% alcohol for 1 minute, soak them in 5 wt.% NaOH for 5 minutes to remove pectin, and then immerse them in 3wt. % sodium hypochlorite solution for 20 minutes, washed 2 times with sterile water, then soaked in normal temperature water for 5 hours, then heat-treated with humidity > 95% and hot and humid air at a temperature of 50 degrees for 40 minutes, then removed the seed coat, and sowed in the seedlings containing 0.2g In the MS medium of / L enrofloxacin sodium, 0.1g / L sulfadimethoxine sodium, 0.02 grams of trimethoprim sodium, 1 mL of azone, and 1 gram of sodium lignosulfonate, the seedlings grow to After 5 cm high, transfer to the sterilized nutrient soil an...

Embodiment 2

[0036] Firstly, the content of Huanglongbing bacteria in the infected mother tree was measured, and the data of month 0 were marked.

[0037] Then it was detoxified, and all steps were carried out in a 100-mesh insect-free net room. The specific steps were as follows: take the seeds of the mother tree and soak them in 70% alcohol for 1 minute, soak them in 5 wt.% NaOH for 5 minutes to remove pectin, and then soak them in 3 wt. .% sodium hypochlorite solution for 20 minutes, washed with sterile water for 3 times, then soaked in water at room temperature for 10 hours, then heat-treated with humidity > 95% and hot and humid air at 55 degrees for 60 minutes, then removed the seed coat, and sowed in seedlings containing 0.6 In the MS medium of g / L enrofloxacin sodium, 0.4g / L sulfadimethoxine sodium, 0.1 g of dimethoprim sodium, 5 mL of azone, and 2 g of sodium lignosulfonate, the seedlings After growing to a height of 5 cm, move it to the sterilized nutrient soil for cultivation, t...

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Abstract

The invention provides a method for protecting and restoring germplasm of citrus-Hunglongbing-infected citrus according to a two-step method. The method comprises the steps of soaking seeds into alcohol with concentration of 70% for one minute, soaking the seeds into NaOH with concentration of 5% for eliminating pectin, then soaking the seeds into sodium hypochlorite solution with concentration of 3% for 20 minutes, cleaning with sterile water by 2-3 times, then immersing the seeds in normal-temperature water for 5-10 hours, then performing thermal treatment on the seeds by means of wet-and-hot air with temperature of 50-55 DEG C for 40-60 minutes, then removing seed shells, sowing the seeds into an MS medium in which enrofloxacin sodium and sulfadimethoxine sodium antibiotic are added, transplanting seedlings into disinfected nutrient soil after the seedlings are grown to a height of 5cm, and grafting the newly grown branch from a nursery stock as a scion on a stock seedling which is grown from a stock seedling which is processed in the same way. According to the method of the invention, the antibiotic composition which comprises the enrofloxacin sodium and the sulfadimethoxine sodium antibiotic is utilized for reasonable mixing; and a two-step method which comprises thermal treatment and adding the antibiotic is firstly utilized for performing sterilization on the citrus-Hunglongbing-infected citrus, thereby realizing higher sterilization effect.

Description

technical field [0001] The invention relates to a two-step method for preserving citrus germplasm infected with Huanglongbing, belonging to the technical field of disease prevention and control. Background technique [0002] Citrus huanglongbing is the most devastating disease of citrus production in the world. After the citrus is infected with the disease, the economic life is shortened sharply, the yield is greatly reduced, the fruit quality is poor, and the economic loss is huge. In recent years, the spread of citrus greening disease has become wider and wider, and the damage has been intensified. It has been widely distributed in more than 50 countries and regions in Asia, Africa, Oceania, South America and North America, and hundreds of millions of citrus trees have been destroyed. The disease has also spread widely in the citrus producing areas of Guangdong, Guangxi, Fujian, Hainan and Taiwan, and successively occurred in Zhejiang, Guizhou and Hunan. 11 of the 19 prov...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G17/00A01G1/06A01G31/00A01C1/08A01N43/60A01N43/54A01P1/00
CPCA01G17/005A01C1/08A01G2/30A01G24/00A01G31/00A01N43/54A01N43/60A01N2300/00
Inventor 程保平彭埃天赵弘巍宋晓兵凌金锋陈霞
Owner PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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