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Molecular marker AhyWptc closely linked with white seed coat of peanut and application of molecular marker AhyWptc

A technology of molecular markers and white seed coats, applied in the field of agricultural biology, can solve the problems of low linkage of white seed coat color traits, difficulty in accurate and convenient application of markers, etc., to improve breeding efficiency, reliable results, and high credibility Effect

Active Publication Date: 2022-05-24
VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The above research has laid the foundation for the identification and molecular breeding of peanut white testa genotypes. Due to the low linkage between the previously developed markers and white testa color traits, it is difficult to accurately and conveniently apply these markers to peanut genetics and molecular breeding research. middle

Method used

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  • Molecular marker AhyWptc closely linked with white seed coat of peanut and application of molecular marker AhyWptc
  • Molecular marker AhyWptc closely linked with white seed coat of peanut and application of molecular marker AhyWptc
  • Molecular marker AhyWptc closely linked with white seed coat of peanut and application of molecular marker AhyWptc

Examples

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Effect test

Embodiment 1

[0032] Example 1 The localization of the color gene controlling the peanut white seed coat and the design of the molecular marker AhyWptc closely linked to the peanut white seed coat

[0033] In order to locate and control the peanut white seed coat color gene, the inventors used the white seed coat peanut variety (female parent) to cross the pink seed coat peanut variety (male parent) in the early stage to construct a segregating population FZ (FZ001×JH8). Discover the harvested hybrid F 1 The seeds are all white, which is consistent with the color of the female parent, and conforms to the genetic rule of "the seed coat is the integument developed into the seed coat, and the color of the seed coat is consistent with the maternal genotype", that is to say, F 2 Progeny plants exhibit trait segregation, in F 3 It can only be displayed in the seed coat of the next generation. As shown in Table 1, genetic analysis indicated that peanut seed coat color is controlled by a single g...

Embodiment 2

[0040] Example 2 Using molecular marker AhyWptc to rapidly cultivate new high-yielding peanut varieties with white seed coat

[0041] 2.1 Experimental subjects

[0042] The seed coat color of the FZ001 peanut cultivar is white, and the seed coat color of the G06-G peanut cultivar is pink. Taking G06-G as the research object, it was genetically improved, and its seed coat color was improved to white while maintaining its high yield.

[0043] 2.2 Experimental steps

[0044] (1) Hybridization

[0045] The powdered peanut G06-G was used as the female parent, and the white-skinned peanut FZ001 was used as the male parent.

[0046] The specific hybridization method is as follows: in the early flowering period of peanuts, there are fewer flowers. In order to make the hybridization time of a combination relatively concentrated, the flowers opened by the female parent G06-G are removed; It is to remove the stamens in the orange unopened flower buds of the female parent after five o...

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Abstract

The invention discloses a molecular marker AhyWptc closely linked with white seed coats of peanuts and application of the molecular marker AhyWptc, and relates to the technical field of agricultural biology. A specific primer pair of the molecular marker AhyWptc comprises a forward primer as shown in SEQ ID NO: 1 and a reverse primer as shown in SEQ ID NO: 2. By utilizing the molecular marker AhyWptc provided by the invention, the seed coat color of a next-generation harvested material can be determined in advance by detecting the DNA of a cotyledon, and the molecular marker AhyWptc is applied to molecular breeding and peanut variety improvement, so that the breeding time can be greatly shortened, the breeding efficiency can be improved, and more excellent peanut germplasm resources can be obtained within shorter time.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a molecular marker AhyWptc closely linked with peanut white seed coat and its application. Background technique [0002] Peanut is an important oil and economic crop in my country, and an important source of vegetable oil and protein. Peanuts are rich in fatty acids, protein, amino acids, dietary fiber, vitamin E and minerals (calcium, iron, zinc, selenium) and other trace elements, and also contain a large number of physiologically active substances such as resveratrol and phenolic substances. It has high oxidative stability and is suitable for food and industrial applications (Zhang Zhimeng et al., 2009). The seed coat colors of peanuts are mainly pink and red, and there are fewer varieties of peanuts with white seed coats. The extracted oil from white-skinned peanuts is light yellow and transparent, and the color remains unchanged after heating, and no decolorizatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11A01H1/04
CPCC12Q1/6895C12Q1/6858A01H1/04C12Q2600/13C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 夏晗黄怀玲邓丽王兴军赵传志任丽马婧田锐铮张宪英侯蕾李阳
Owner VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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