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Lepista nuda strain and sporocarp cultivation method thereof

A technology of lilac mushrooms and fruiting bodies, applied in the field of microorganisms, can solve the problems of many uncontrollable factors and long fruiting time, and achieve the effect of enriching variety resources and enriching the vegetable basket

Active Publication Date: 2018-11-09
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 1994, there was a report on the domestication and cultivation of the natural edible fungus Lilac mushroom in the tea garden (Dai Caiyun and Cui Xiaoming, 1994 Guizhou Tea 1: 24). It was reported that it was cultivated in the open field of the tea garden. After 7 months, the mushrooms were produced. The method used in the report was open field cultivation. There are many uncontrollable factors, and the fruiting time is long, so far no promotion has been seen

Method used

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  • Lepista nuda strain and sporocarp cultivation method thereof
  • Lepista nuda strain and sporocarp cultivation method thereof
  • Lepista nuda strain and sporocarp cultivation method thereof

Examples

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Effect test

Embodiment 1

[0047]Example 1. Acquisition and Identification of Lilac Mushroom DCH560 (CGMCC No.15376)

[0048] The bacterial strain DCH560 of the present invention is isolated from natural Lilac mushroom specimens (Lilac mushroom specimens were collected from Kuandian Manchu Autonomous County, Dandong, Liaoning, China in 2016), through the observation of its colony and hyphae morphological characteristics, combined with nuclear rDNA-ITS sequencing, the The strain was classified and identified.

[0049] 1. Morphological features

[0050] Bacterial strain DCH560 was cultured on potato dextrose agar medium (PDA) at 20°C for 2 weeks. The diameter of the colony was about 5cm. See figure 1 and figure 2 ). The hyphae are septate, transparent, with clavicle joints, 1.2-5μm in diameter, no asexual spores were observed.

[0051] 2. Molecular biological characteristics

[0052] The rDNA-ITS sequence is used as a barcode (Schoch, C.L. et al. Nuclear ribosomal internal transcribed spacer (ITS) ...

Embodiment 2

[0056] Embodiment 2, the method for the fruiting body cultivation of Lilac mushroom DCH560 (CGMCC No.15376)

[0057] 1. Cultivation process

[0058] The cultivation process of the fruiting body of Lilac mushroom DCH560 (CGMCC No.15376) is as follows: mother seed preparation→original seed preparation→cultivar seed preparation→inoculation and germination→covering soil→fruiting management→fruiting→harvesting.

[0059] (1) Preparation of mother species: inoculate the hyphae of Lilac mushroom DCH560 (CGMCC No.15376) after tissue separation and domestication into the mother species medium (test tube slant), 15-25°C (such as 20-23°C) ) in the dark and cultivated until the mycelium is covered with the medium, and the obtained mycelium is used as the mother species ( image 3 ).

[0060] (2) Preparation of the original seed: transfer the mother seed obtained in step (1) to the original seed medium, and the inoculum size is to evenly insert 6-10 cells with a diameter of 1.0 cm in the ...

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Abstract

The invention discloses a lepista nuda strain and a sporocarp cultivation method thereof. The invention provides Lepista nuda(Lepista nuda DCH 560), and the deposit number is CGMCC No. 15376. The Lepista nuda DCH 560 (CGMCC No. 15376) is collected from a specimen isolated from the specimen of Dandong Kuandian Manchu Autonomous County, Liaoning Province, China. The strain can be obtained by artificial domestication. The sporocarp cap obtained by cultivation is purple, the flesh is thicker, the pleats are purple, and it has a strong aroma, which is consistent with the wild sporophore. The invention has obtained thesporophore of the lepista nuda and developed a new rare edible fungus, which is of great significance for protecting the germplasm resources of the species, enriching the edible mushroom variety resources, enriching the vegetable basket and the like. At the same time, the bacterium is a typical grass rot fungus and the crop stalks can be utilized, which has ecological significance.

Description

technical field [0001] The invention belongs to the technical field of microbes, and relates to a lilac mushroom strain and a fruiting body cultivation method thereof. Background technique [0002] Lilac mushroom Lepista nuda (Bull.) Cooke, also known as naked mushroom and amethyst mushroom, belongs to the family Agaricaceae, Tricholomaceae, and the genus Agaricus. Lilac Mushrooms. Lilac mushroom has a pleasant color, strong fragrance, tender and sweet, and excellent taste. It is known as "one family eats its taste, and ten families smell its fragrance". It is as popular as truffles and boletus in Europe, and is regarded as For the best ingredients. [0003] As a rare edible and medicinal fungus, lilac mushroom has high research and development value, but so far the main source is wild collection, its natural resources are limited, and its quality cannot be guaranteed. Although some units have carried out the domestication and cultivation of Lilac mushroom, there is no re...

Claims

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Application Information

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IPC IPC(8): A01G18/00
CPCA01G18/00
Inventor 董彩虹刘晴刘奇正董浩
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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