SNP molecular marker for bright green cucumis sativus fruits, normal fruit thorn tumors and no fruit frost and application of SNP molecular marker

A technology of molecular markers and cucumbers, applied in the field of agricultural biology, can solve problems such as no commodity value

Pending Publication Date: 2022-01-07
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mutants reduce the presence of fruit cream to a certain extent, but the mutant type has basically no commercial value for consumers who prefer strong michael flavor

Method used

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  • SNP molecular marker for bright green cucumis sativus fruits, normal fruit thorn tumors and no fruit frost and application of SNP molecular marker
  • SNP molecular marker for bright green cucumis sativus fruits, normal fruit thorn tumors and no fruit frost and application of SNP molecular marker
  • SNP molecular marker for bright green cucumis sativus fruits, normal fruit thorn tumors and no fruit frost and application of SNP molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Acquisition of bright green fruit, normal fruit thorn tumors and no fruit frost-type cucumber

[0039] This example provides a method for obtaining a cream-type cucumber with bright green fruit, normal fruit thorn tumors and no fruit, and the steps are as follows.

[0040] Using Tianjin Jinlu and Xintaimici cucumber inbred lines as parent materials, after conventional compound crossing, in the segregation population of offspring, there were recombined plants with fresh cucumber traits of light green fruit and less fruit surface, and then three generations of self-crossing and continuous selection.

[0041] In the planting population of selfed offspring, the inventor found a cucumber plant with bright fruit and no fruit frost. Its main characteristics are as follows: starting from a true leaf, it has exactly the same leaf characteristics as its separated parent, and this characteristic is maintained until the flowering and fruiting period. Four days after flo...

Embodiment 2

[0045] Example 2 Genetic analysis of cucumber material R59

[0046] This example provides the genetic analysis of the cucumber material R59 obtained in Example 1. Cucumber R59 is a plant with bright green fruit skin and no fruit frost obtained from Tianjin Jinlu and Xintaimici inbred lines.

[0047] (1) The R59 material was used as one of the parents, and the wild-type cucumber inbred line was used as the other parent to configure reciprocal hybrids (the female parent and The wild-type inbred line is crossed to be orthogonal, and the fruit is bright green, the fruit prickly tumor is normal, and the fruit is frosty and dense. The male parent is crossed with the wild-type inbred line (reverse cross), and harvested separately to obtain the F1 generation.

[0048] Plant the F1 generation of positive and negative hybrid combinations, observe the fruit peel brightness, thorn tumors and fruit frost of the F1 generation plants; and select half of the F1 generation plants in each hybr...

Embodiment 3

[0055] Example 3 Genotype analysis of cucumber material R59

[0056] This example analyzes the genotype of the cucumber material R59 obtained in Example 1. The result is as follows.

[0057] Compared with the wild type, the nucleotide at No. 12733267 of the third chromosome of the cucumber material R59 was mutated from C to T, and the mutation here brought the cucumber fruit from the multi-fruited cream dark green dense thorn type to the fruitless cream Bright green dense thorn type.

[0058] Based on the genetic analysis results in Example 2, the bright green and fruitless frost character of the fruit is controlled by a single recessive nuclear gene, and the gene where the mutation is located is named csfbc. The base composition of position 12734020.

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Abstract

The invention discloses an SNP molecular marker for bright green cucumis sativus fruits, normal fruit thorn tumors and no fruit frost and an application of the SNP molecular marker. According to the invention, the fact that the cucumis sativus fruit color and the fruit frost character are controlled by a single recessive genic gene csfbc is found for the first time, and the csfbc is a gene at a base position of 12731768-12734020 on a third chromosome of a cucumis sativus genome. Compared with a csfbc gene of common multi-fruit-frost dark-green dense-thorn cucumis sativus, the mutant csfbc refers to that a basic group at the 12733267th position of the third chromosome of the cucumis sativus genome is mutated into T from C. The cucumis sativus mutant csfbc provided by the invention enriches cucumis sativus variety resources, and provides ideal genetic resources for genetic breeding of cucumis sativus. The fruit-frost-free dense-thorn cucumis sativus with bright green fruits and normal thorn knots conforms to the purchase habits and consumption preferences of consumers, and a good variety with value-added value is provided for income increase of farmers.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and relates to plant fruit appearance quality and a breeding method thereof, in particular to a SNP molecular marker of bright green cucumber fruit, normal fruit thorn tumors, and no fruit frost and its application. Background technique [0002] Cucumber (cucumis sativus L.) has experienced at least 3000 years from wild species to people's cultivation, and has formed different cultivars so far. Cucumber is one of the ten most important vegetable crops in the world. It uses the immature fruit as the product organ, which is mainly used for fresh food. For producers, the market is an important place to reflect the added value of cucumbers. Generally speaking, less or no fruit frost and its bright skin are one of the important characteristics to evaluate the good quality of cucumber fruit, and it is also the main factor affecting consumers' purchase. At present, the dominant cucumber vari...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 刘兴旺任华中张亚琦高一铭翟许玲冯钟萱
Owner CHINA AGRI UNIV
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