Maple-leaf-type cucumber plant
一种枫叶、黄瓜的技术,应用在枫叶型黄瓜植物领域,能够解决未发现枫叶型黄瓜植物等问题,达到群体通风透光性强、减少病害的发生、减少农药的使用的效果
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Embodiment 1
[0070] Example 1 Genetic Analysis Test of Maple Leaf Cucumber Plant Mutant CC81221
[0071] (1) Test materials
[0072] The inventor discovered a maple leaf in the inbred line material of the offspring of multiple compound crosses of farm varieties Tangshan Qiugua, Baiye San and European fresh cucumber varieties planted in the Shangzhuang Experimental Greenhouse, Haidian District, Beijing in the spring of 2012 type mutant, and named it CC81221, its photo is shown in figure 2 , image 3 .
[0073] The plant of the genus Cucumis (Cucumis sativus L.) CC81221 has been preserved on October 12, 2015 in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures (the preservation address is: Courtyard 3, No. 1 Beichen West Road, Chaoyang District, Beijing No. Institute of Microbiology, Chinese Academy of Sciences), the deposit number is: CGMCC No.11285.
[0074] Several common leaf-shaped cucumbers provided by Beijing Beinong Sanyi Cucumber Ec...
Embodiment 2
[0099] Embodiment 2 is cultivated to have maple leaf type cucumber plant variety by hybridization method
[0100] Cucumber plants with maple-leaf leaves were bred by using cucumber plants with maple-leaf leaves CC81221 or cucumber inbred lines, F1 hybrids or hybrids derived therefrom as maple-leaf-leaf donors. Therefore, any cucumber plant with maple-leaf leaves selected by the method of the present invention and the cucumber plant with maple-leaf type CC81221 or derived from it all belong to the scope of the present invention.
[0101] (1) On May 25, 2012, the test was carried out in the experimental shed in Haidian, Beijing. The experimental shed was closed and isolated with insect-proof nets. The cucumber material CC81221 with maple leaf-shaped leaves was used as one of the parents, and the cucumber ecological breeding with Beijing Beinong Sanyi was carried out respectively. Three different types of common leaf inbred lines provided by the Science and Technology Center: den...
Embodiment 3
[0109] Example 3 Utilize the backcross method to select and breed maple leaf type leaf cucumber variety test
[0110] The main purpose of the backcrossing method used in the present invention is to alter or replace the leaf shape of the original variety while retaining substantially all other desirable genetics, as well as the desirable physiological and morphological composition of the original variety.
[0111] The maple-leaf type leaf is a characteristic controlled by a single recessive nuclear gene. In this case, the test of the offspring must be introduced in the backcrossing step, that is, the phenotypic test is carried out on the corresponding selfed offspring of each backcrossing generation, In order to determine which backcross plants carry the recessive gene encoding maple-type leaves.
[0112] The backcrossing process can be simplified and accelerated by using molecular markers such as SSR, RFLP, SNP or AFLP markers.
[0113] (1) On May 25, 2012, it was carried out...
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