Quantitative character gene pyramiding breeding method for pinctada fucata

Abstract

The invention discloses a quantitative character gene pyramiding breeding method for pinctada fucata. The method comprises the following steps of: collecting at least three pinctada fucata groups from different sources, constructing F1 generation diallel crossing families, evaluating every each family after cultivating for one year, computing an average breeding value, and selecting families of which the breeding values are ranked at first 50 percent for serving as candidate families for constructing a core breeding family, wherein every each combination comprises at least ten families; screening female and male individuals from the candidate families, selecting female and male individuals which are ranked at first 100 places in sizes for measuring, computing individual breeding values, and sequencing; selecting female and male individuals with highest individual breeding values from families with highest female and male breeding values for mating respectively, selecting female and male individuals of which the individual breeding values are ranked at first places from families of which the female and male breeding values are ranked at second places for mating, and so on, and constructing an F2 generation core breeding family; and constructing multiple generations of families including F3, F4, F5 and the like according to the method for breeding generation after generation to over five generations. Through breeding according to the method, the genetic improvement of every each generation can be over 10 percent, the inbreeding coefficient can be controlled at a set level, and inbreeding depression is avoided.

Description

technical field

[0001] The invention belongs to the field of aquatic breeding, and in particular relates to a gene aggregation breeding method for quantitative traits of Hepu pinnacle oyster. Background technique

[0002] Hepu mother-of-pearl ( Pinctada fucata ) is an important marine cultured shellfish and the main mother oyster for pearl production. The well-known Hepu pearl, namely "South Pearl" is produced by this shellfish. Hepu pearl oysters mainly live in tropical and subtropical sea areas. The research and production of Hepu pearl oyster cultured pearls in my country began in the middle of the twentieth century. The successful artificial breeding of pearls in 1958 and the success of artificial seedling breeding in 1965 marked the entry of Hepu pearl oyster into the era of full artificial breeding, that is, the use of artificially propagated seedlings for cultivation. After breeding, mature individuals are selected and artificially propagated to form a closed cycle s...

Examples

Embodiment

[0019] A Hepu pearl oyster quantitative trait gene aggregation breeding method, comprising the steps of:

[0020] (1) Collect more than 200 Hepu mother-of-pearl parents from sea areas or farms in Sanya, Beihai, and Xuwen respectively, select individuals with correct shape, integrity, and no disease, and measure the shell length, shell height, and shell width And body weight and other indicators, the F1 generation basic family was constructed by using the diallel cross method with a ratio of male to female at 1:1. Each combination constructs more than 10 families, and the total number of families is 90. The seedling breeding conditions of all families should be controlled under the same conditions as far as possible. When the seedlings reach about 1mm and emerge, they will be moved to the sea area to be cultured in net cages, and the conditions such as stocking density are the same. Other breeding management measures are the same as conventional production.

[0021] (2) Famil...

technical field

[0001] The invention belongs to the field of aquatic breeding, and in particular relates to a gene aggregation breeding method for quantitative traits of Hepu pinnacle oyster. Background technique

[0002] Hepu mother-of-pearl ( Pinctada fucata ) is an important marine cultured shellfish and the main mother oyster for pearl production. The well-known Hepu pearl, namely "South Pearl" is produced by this shellfish. Hepu pearl oysters mainly live in tropical and subtropical sea areas. The research and production of Hepu pearl oyster cultured pearls in my country began in the middle of the twentieth century. The successful artificial breeding of pearls in 1958 and the success of artificial seedling breeding in 1965 marked the entry of Hepu pearl oyster into the era of full artificial breeding, that is, the use of artificially propagated seedlings for cultivation. After breeding, mature individuals are selected and artificially propagated to form a closed cycle s...

Examples

Embodiment

[0019] A Hepu pearl oyster quantitative trait gene aggregation breeding method, comprising the steps of:

[0020] (1) Collect more than 200 Hepu mother-of-pearl parents from sea areas or farms in Sanya, Beihai, and Xuwen respectively, select individuals with correct shape, integrity, and no disease, and measure the shell length, shell height, and shell width And body weight and other indicators, the F1 generation basic family was constructed by using the diallel cross method with a ratio of male to female at 1:1. Each combination constructs more than 10 families, and the total number of families is 90. The seedling breeding conditions of all families should be controlled under the same conditions as far as possible. When the seedlings reach about 1mm and emerge, they will be moved to the sea area to be cultured in net cages, and the conditions such as stocking density are the same. Other breeding management measures are the same as conventional production.

[0021] (2) Famil...