Tissue culture and propagation method and inductive culture mediums for Chongzuo camellia nitidissima

A technology of induction medium and medium, which is applied in the field of Chongzuo golden camellia tissue culture propagation method and its induction medium, can solve the problems of narrow natural distribution range, low rooting rate of cuttings, limited quantity, etc., and solve the problem of growing wild resources. Reduce and solve the effect of low natural reproduction rate

Inactive Publication Date: 2013-03-20
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The natural distribution range of Chongzuo golden camellia is narrow and the quantity is limited. Due to the thin and weak branches, the low rooting rate of cuttings, and the difficulty of asexual reproduction, it is difficult to meet the market demand of garden flowers.
So far, although there are some reports on the plant tissue culture of Camellia japonica at home and abroad, there is no report on the tissue culture of Camellia japonica in Chongzuo

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Cluster bud induction

[0024] Take the young seeds of Chongzuo Golden Flower Tea, first wash the surface dirt with detergent, then rinse with running water for 30 minutes, then treat with 75% ethanol on an ultra-clean workbench for 30 seconds, rinse with sterile water twice, and then use 0.1HgCl 2 Disinfect for 20 minutes, rinse with sterile water 5 times; then use 0.2% HgCl 2 Sterilize for 15 minutes, rinse with sterile water for 6 times; absorb the water with sterile filter paper, pick out the milky white embryos and plant them on the medium with modified MS supplemented with 6-BA 4mg / L, IAA 0.6mg / L, and pH adjusted to 5.0 Above, at a temperature of 25±1℃, and an illumination time of 12h.d -1 , Under the condition of 2000lx light intensity, cultured for 65 days, an average of 15.2 times the cluster buds are obtained. The cluster buds are thicker, greener, and grow slightly slower.

[0025] (2) Rooting induction

[0026] When the clumping buds grow to 3~4cm, the clumping...

Embodiment 2

[0028] (1) Cluster bud induction

[0029] Take the young seeds of Chongzuo Golden Flower Tea, first wash away the dirt on the surface with detergent, then rinse with running water for 30 minutes, and then treat with 75% ethanol on an ultra-clean workbench for 40 seconds, rinse with sterile water twice, and then use 0.1HgCl 2 Disinfect for 25 minutes, rinse with sterile water 5 times; then use 0.2% HgCl 2 Sterilize for 15 minutes and rinse with sterile water for 6 times; blot the explants with sterile filter paper, pick out the milky white embryos and plant them with modified MS with 6-BA 3mg / L, IAA 0.70mg / L, and pH adjusted to 5.5 On the culture medium, the temperature is 25±1℃, and the light time is 12h.d -1 , Under the condition of 2000lx light intensity, cultivated for 60 days, an average of 18 times the cluster buds will be obtained, the cluster buds are thick and strong, the leaves are thick and green, and the growth is good.

[0030] (2) Rooting induction

[0031] When the clum...

Embodiment 3

[0033] (1) Cluster bud induction

[0034] Take the young seeds of Chongzuo Golden Flower Tea, first wash away the dirt on the surface with detergent, then rinse with running water for 30 minutes, and then treat with 75% ethanol on an ultra-clean workbench for 40 seconds, rinse with sterile water twice, and then use 0.1HgCl 2 Sterilize for 30 minutes, rinse with sterile water 5 times; then use 0.2% HgCl 2 Sterilize for 20 minutes, rinse with sterile water for 6 times; absorb the explants with sterile filter paper, and plant them on a medium with modified MS supplemented with 6-BA 2mg / L, IAA 0.8mg / L, and pH adjusted to 5.8. The temperature is 25±1℃, the light time is 12h.d -1 ,Under the condition of 2000lx light intensity, cultivated for 55 days, an average of 20 times the cluster buds will be obtained. The cluster buds are thicker, the leaves are dark green, and the growth is faster.

[0035] (2) Rooting induction

[0036] When the clump buds grow to 3~4cm, the clump buds are divided ...

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PUM

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Abstract

The invention discloses a tissue culture and propagation method and inductive culture mediums for Chongzuo camellia nitidissima. According to the method, the improved MS, 6-benayl aminopurine (6-BA) and 3-indoleacetic acid (IAA) are used as an inductive culture medium for the cluster buds of Chongzuo camellia nitidissima; and the combination of the two growth hormones can be used for activating acting enzymes in the body cells of Chongzuo camellia nitidissima, and promoting the germination and the growth of the explants of Chongzuo camellia nitidissima in a proper concentration and a weakly acidic environment; the thick and strong cluster buds can be formed after the culturing for 55 days, and the propagation rate is increased by 20 times. According to the method disclosed by the invention, 1/2 improved MS and indolebutyric acid (IBA) or 1/2 improved MS and napthaleneacetic acid (NAA) are further used as an inductive culture medium for rooting, and capable of effectively promoting the single seedlings of Chongzuo camellia nitidissima to root to form new plants. Via the tissue culture and propagation method applying the three inductive culture mediums for Chongzuo camellia nitidissima, Chongzuo camellia nitidissima can be rapidly and effectively propagated, thus solving the problems that wild resources are diminished gradually and the natural propagation rate of Chongzuo camellia nitidissima is low, and effectively protecting the precious plant genetic resource which is rare in the world.

Description

Technical field [0001] The invention relates to the field of plant tissue culture and propagation, in particular to a method for tissue culture and propagation of Chongzuo Camellia tea and an induction medium. Background technique [0002] C.chungtsoensis SYLiang et LDHuang, an evergreen shrub, is a recently discovered rare variety of camellia. It is the second variety of camellia that blooms in four seasons after azalea red camellia. , It is not only the brightest yellow in the Camellia family, but it also blooms throughout the year. Chongzuo Jinhua tea is only distributed in a small amount in the local limestone low mountain area of ​​Chongzuo, Guangxi. This species is similar to the lemon golden flower tea, the difference is that the flower is large and dark yellow, the petals are 13-16, and it blooms in all seasons. Chongzuo Golden Camellia has evenly distributed flowers and dense branches. It can grow normally under full light conditions. It has high ornamental value and h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 杨舒婷王华新林茂唐遒冥龚建英孙利娜杜铃廖美兰陈尔陈宝玲李娜黄欣汪小玉杜禹
Owner GUANGXI FORESTRY RES INST
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