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Method for breeding adventitious buds and adventitious roots by cuttage induction of primulina tabacum hance petioles

A technology of primrose and petiole cuttings, applied in the field of plant breeding, can solve problems such as unreported, and achieve the effects of an effective reproduction system, an effective plant regeneration system, and the elimination of endangered status.

Inactive Publication Date: 2012-10-10
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technique of breeding and rooting with the petiole cuttings of Primula lettuce has not been reported at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] On February 28th, take the petiole with leaves of 2.0cm long adult Primulus moss as explants, and the explants are first injected with 1.0mg L -1 After soaking in IBA aqueous solution for 30 minutes, insert the oblique cutting of the petiole into the mixed substrate of river sand, vermiculite and perlite (1:1:1, v / v / v) (put in a pot: length × width × height=30cm ×40cm×10cm), the leaves stand on the surface of the substrate, the growth environment of cuttings is sunshade, ventilated on all sides, and ventilated greenhouses, the control environment temperature is between 18~32°C, and the relative humidity is controlled between 50~95%. , watering with moisture retention two days apart, after cultivating for 50 days, the rooting rate was 90%, and the average number of adventitious buds was 8.8. The clustered adventitious buds induced by petioles were divided into individual buds and transplanted in the matrix of sandstone, vermiculite and perlite (1:1:1, v / v / v). Under norm...

Embodiment 2

[0016] On March 10th, take the 3.0cm-long petiole with leaves of Primula moss as explants, first cut in the matrix mixed with river sand and perlite (1:1, v / v), and the leaves stand on the matrix On the surface, spray 1.0 mg L of each cutting pot (length × width × height = 30cm × 40cm × 10cm) -1 10ml of IBA aqueous solution was used twice. The growth environment of cuttings is in a greenhouse with sunshade, ventilation on all sides, and ventilation. The temperature of the environment is controlled between 18-32°C, the relative humidity is controlled between 50-95%, and water is kept every two days to keep it moist. After culturing for 55 days, the rooting rate was 65%, and 4.5 adventitious buds were induced on average. The clustered adventitious buds induced by petioles were divided into individual buds and transplanted in the matrix of sandstone and perlite (1:1, v / v). Under normal conditions, the survival rate was 90% after 20 days.

Embodiment 3

[0018] On April 15th, take the petiole with leaves of Primula moss 5.0cm long as explants, and the explants are first mixed with 5.0mgL -1 After soaking in BA aqueous solution for 1 hour, the petiole cuttings were then placed in a mixed substrate of river sand and perlite (1:1, v / v) (in a pot: length×width×height=30cm×40cm×10cm) , the leaves stand on the surface of the substrate, the growth environment of cuttings is sunshade, ventilated on all sides, and ventilated greenhouses, the temperature of the environment is controlled between 18 ~ 32 ° C, the relative humidity is controlled between 50 ~ 95%, water every day to moisturize , after culturing for 60 days, the rooting rate was 51%, and the surviving leaves had an average of 8.3 adventitious buds. The clustered adventitious buds induced by petioles were divided into individual buds and transplanted in the matrix of sandstone and perlite (1:1, v / v). Under normal conditions, the survival rate was 90% after 20 days.

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Abstract

The invention discloses a method for breeding adventitious buds and adventitious roots by cuttage induction of primulina tabacum hance petioles. The method comprises the following steps of: selecting proper explants, treating the explants by using a plant growth regulator, inserting the explants into a proper matrix, culturing the explants under proper conditions to induce the adventitious buds and the adventitious roots, and transplanting seedlings to form plants. The method is simple, convenient, fast and effective, and by the method, a large batch of seedlings can be bred in short time andthe plants can be grown further. To better protect and use the rare threatened plant, a set of simple, fast and effective breeding and plant regeneration system is established, so that the national first batch of level 1 species are effectively protected and bred and released from the endangered state, and a good foundation is laid for further protecting and using the species in future.

Description

Technical field: [0001] The invention belongs to the technical field of plant breeding, and in particular relates to a breeding method for inducing adventitious buds and adventitious roots by cutting the petiole of primula moss. Background technique: [0002] Primulina tabacum Hance (Primulina tabacum Hance) is a small perennial herb of Gesneriaceae. Since American Henry discovered the rare plant Primulina tabacum Hance on the stone wall of the Lianjiang River Basin in Lianzhou, northern Guangdong in 1881, it has been The mystery disappeared for 125 years. Until a few years ago, relevant domestic media reported that the endangered plant Primula was discovered again in Lianzhou. Its discovery is undoubtedly of great academic value to the study of Lingnan paleoclimate, soil and animal and plant evolution. Due to the special living environment requirements, narrow geographical distribution and small population of this species, it has been identified by the state as the first ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/00A01G31/00
Inventor 马国华吕金凤胡玉姬张新华
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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