4 results about "Cymbidium sinense" patented technology

The application discloses a kind of SSR molecular marker primer for identifying Cymbidium varieties, and its application, the Cymbidium includes Cymbidium kanran, Cymbidium ensifolium, Cymbidium goeringii, Cymbidium sinense, Cymbidium devonianum, Cymbidium lancilobatum, Cymbidium kanran, hybrid orchid and hybrid Cymbidium. The SSR molecular marker includes SSR1-SSR23, and the primer sequence is shown as SEQ ID NO.1-SEQ ID NO.46 in the sequence table. The screening method of the SSR molecular marker primer is also included, which comprises the following steps of total DNA extraction of Cymbidium, SSR site selection and primer design based on Cymbidium reference genome, PCR amplification, polyacrylamide gel electrophoresis primary screening and ABI3730XL gene analyzer secondary screening. The application also provides a composition comprising the primer and its application in Cymbidium genetic relationship analysis, DUS specificity test and molecular marker assisted breeding. The SSR molecular marker primer provided by the application has the characteristics of high polymorphism, large quantity, low cost, easy reading, and is not limited by environment, and can quickly and accurately construct the DNA fingerprint of Cymbidium varieties.

The invention discloses a method for obtaining distant intergeneric hybrids of orchid. The method provided by the invention comprises the following steps: carrying out intergeneric distant hybridization on cymbidium sinense 'Jianxiaoji', cymbidium ensifolium 'Qingshan Yuquan', cymbidium ensifolium 'Xianshan Red' and winter impatiens of cymbidium as male parents, and culturing hybrid seeds into seedlings at the stages of sterile sowing, germination, differentiation and rooting and seedling strengthening, so as to obtain hybrid seedlings with complete plants. According to the method, the distant intergeneric hybrid seedlings obtained through specific hybridization conditions are high in hybridization fruit setting rate, high in germination success rate, short in germination starting time and good in seedling forming effect. According to the method provided by the invention, the success rate of distant hybridization of the orchid can be increased, the affinity obstacle of distant hybridization of the orchid genus and the orchid genus is overcome, the limitation that the genetic relationship between the orchid genus and the orchid genus is extremely far and natural geographical isolation exists is broken through, new germplasm is provided for planting and appreciating the orchid, and the method has important significance.

This invention discloses a gene for regulating the growth of Cymbidium goeringii. CsARF4 Its coding sequence and applications belong to the field of plant molecular biology. This invention's analysis revealed that... CsARF4 The highest expression was observed in the roots of Cymbidium goeringii; it was also observed at different flower development stages in Cymbidium goeringii. CsARF4 The expression trend is a gradual decrease; in the tissue parts of mature Cymbidium flowers, CsARF4 The expression is highest in the petals. This invention, through genetic engineering technology, discovered that the aforementioned gene regulating the growth and development of *Cymbidium goeringii* is overexpressed in *Cymbidium sinense*, altering plant growth traits, resulting in increased rhizomes and rapid growth. Therefore, this gene and its encoded protein can be used to study growth and development regulation pathways such as rhizome proliferation, increased budding rate, and promotion of rapid plant growth, as well as for improving growth traits.

The invention discloses a Chinese orchid growth regulation gene CsARF4 as well as a coding sequence and application thereof, and belongs to the field of plant molecular biology. Analysis finds that the expression of CsARF4 in Chinese cymbidium roots is the highest; in different flower development stages of cymbidium sinense, the expression trend of CsARF4 is gradually reduced; in tissue parts of mature flowers of cymbidium sinense, the expression of CsARF4 in petals is the highest. Through a genetic engineering technology, it is found that overexpression of the Chinese cymbidium growth and development regulation gene in cymbidium sinense can change plant growth traits and cause increase and rapid growth of rhizomes, so that the gene and a protein coded by the gene can be used for rhizome proliferation, the budding rate is increased, and the yield of plants is increased. Research on growth and development regulation ways such as rapid growth of plants is promoted, and the method is used for improving growth traits.