12 results about "Cymbidium sinense" patented technology

The invention relates to the technical field of biology, in particular to a CsRBE gene for regulating and controlling lip form of cymbidium sinense, application of the CsRBE gene, protein and a method. The nucleotide sequence of the CsRBE gene is as shown in SEQ ID No. 1. The CsRBE gene provided by the invention can regulate and control the lip form of cymbidium sinense, after the CsRBE gene is silenced, the lip form of cymbidium sinense is flat without bending, the lip width and the fold gap in the middle of the lip are increased, and an important basis is provided for cymbidium sinense breeding.

The application discloses a kind of SSR molecular marker primer for identifying Cymbidium varieties, and its application, the Cymbidium includes Cymbidium kanran, Cymbidium ensifolium, Cymbidium goeringii, Cymbidium sinense, Cymbidium devonianum, Cymbidium lancilobatum, Cymbidium kanran, hybrid orchid and hybrid Cymbidium. The SSR molecular marker includes SSR1-SSR23, and the primer sequence is shown as SEQ ID NO.1-SEQ ID NO.46 in the sequence table. The screening method of the SSR molecular marker primer is also included, which comprises the following steps of total DNA extraction of Cymbidium, SSR site selection and primer design based on Cymbidium reference genome, PCR amplification, polyacrylamide gel electrophoresis primary screening and ABI3730XL gene analyzer secondary screening. The application also provides a composition comprising the primer and its application in Cymbidium genetic relationship analysis, DUS specificity test and molecular marker assisted breeding. The SSR molecular marker primer provided by the application has the characteristics of high polymorphism, large quantity, low cost, easy reading, and is not limited by environment, and can quickly and accurately construct the DNA fingerprint of Cymbidium varieties.

The invention discloses a method for obtaining distant intergeneric hybrids of orchid. The method provided by the invention comprises the following steps: carrying out intergeneric distant hybridization on cymbidium sinense 'Jianxiaoji', cymbidium ensifolium 'Qingshan Yuquan', cymbidium ensifolium 'Xianshan Red' and winter impatiens of cymbidium as male parents, and culturing hybrid seeds into seedlings at the stages of sterile sowing, germination, differentiation and rooting and seedling strengthening, so as to obtain hybrid seedlings with complete plants. According to the method, the distant intergeneric hybrid seedlings obtained through specific hybridization conditions are high in hybridization fruit setting rate, high in germination success rate, short in germination starting time and good in seedling forming effect. According to the method provided by the invention, the success rate of distant hybridization of the orchid can be increased, the affinity obstacle of distant hybridization of the orchid genus and the orchid genus is overcome, the limitation that the genetic relationship between the orchid genus and the orchid genus is extremely far and natural geographical isolation exists is broken through, new germplasm is provided for planting and appreciating the orchid, and the method has important significance.

The present invention discloses the coding sequence of the Cymbidium flowering regulation gene CsFTL1 and its application. The Cymbidium flowering regulation protein CsFTL is characterized in that its amino acid sequence is as shown in SEQ ID NO: 2. The present invention isolated the key flower development gene FTL1 from the flower bud cDNA of the Cymbidium sinense variety 'Xiaoxiang' and named it CsFTL1. Temporal and spatial expression pattern analysis found that the expression of CsFTL1 was the highest in the flowers of Cymbidium sinense; among different flower development stages of Cymbidium sinense, the expression trend of CsFTL1 was that the mRNA accumulation increased rapidly at the stage of flower bud differentiation and then gradually decreased. After heterologously expressing CsFTL1 in Arabidopsis thaliana, it was found that Arabidopsis thaliana showed the phenotype of early flowering. The Cymbidium flowering regulation gene of the present invention can be used for the study of the molecular mechanism of flower organ development in Orchidaceae plants and the improvement of plant flowering traits.

The present invention discloses a coding sequence of a flowering regulatory gene SEP1 of Chinese orchid and its application. The present invention isolates an orchid flower organ development regulatory gene from the flower organ cDNA of the Chinese orchid variety 'Bai Mo'. After increasing its expression level, the flowering period of Arabidopsis thaliana can be significantly advanced, but the flower organ morphological structure is not affected. Through downstream gene expression analysis, it is found that the gene can positively regulate the expression of flowering control genes FT, SOC1, and LFY1, further indicating the role of the gene in promoting plant flowering. The Chinese orchid flowering regulatory gene of the present invention can be used for the study of the molecular mechanism of flowering regulation of orchidaceae plants and the improvement of flowering traits of plants.

1. Name of the Design Product: Umbrella (Quiet Rain of Cymbidium Sinense). 2. Use of the Design Product: A rain gear for sunshading or rain blocking. 3. Design Key Points of the Design Product: The combination of shape, pattern and color. 4. Picture or Photograph Most Indicating the Design Key Points: Top view. 5. The design for which protection is claimed includes color.

This invention discloses a gene for regulating the growth of Cymbidium goeringii. CsARF4 Its coding sequence and applications belong to the field of plant molecular biology. This invention's analysis revealed that... CsARF4 The highest expression was observed in the roots of Cymbidium goeringii; it was also observed at different flower development stages in Cymbidium goeringii. CsARF4 The expression trend is a gradual decrease; in the tissue parts of mature Cymbidium flowers, CsARF4 The expression is highest in the petals. This invention, through genetic engineering technology, discovered that the aforementioned gene regulating the growth and development of *Cymbidium goeringii* is overexpressed in *Cymbidium sinense*, altering plant growth traits, resulting in increased rhizomes and rapid growth. Therefore, this gene and its encoded protein can be used to study growth and development regulation pathways such as rhizome proliferation, increased budding rate, and promotion of rapid plant growth, as well as for improving growth traits.

The invention belongs to the technical field of plant tissue culture, and particularly relates to a cymbidium faberi rhizome tissue culture medium and a tissue culture rapid propagation method. By exploring the novel organic additive, the research result shows that the novel organic additive has a remarkable promoting effect on proliferation and differentiation of cymbidium sinense rhizomes and rooting of aseptic seedlings, and the tissue culture period is 7 months. The iron stick yam enables the multiplication coefficient of the number of rhizomes to reach 7.9, the lotus root enables the number of induced buds of the rhizomes to reach 8.4, and the coconut meat enables the rooting rate of aseptic seedlings to reach 80.2%. Finally, the optimal culture medium formula for the cymbidium faberi rhizome proliferation is determined as follows: MS, 0.5 mg.L <-16 >-BA, 1.0 mg.L <-1 > NAA, 1.0 g.L <-1 > AC, 7.8 g.L <-1 > Ag, 30.0 g.L <-1 > Su and 100.0 g.L <-1 > iron stick yam; the optimal culture medium formula for rhizome differentiation is as follows: MS + 1.0 mg.L <-1 > TDZ + 7.8 g.L <-1 > Ag + 20.0 g.L <-1 > Su + 45.0 g.L <-1 > lotus root; the optimal culture medium formula for rooting of the aseptic seedlings is as follows: MS + 1.5 mg.L <-1 > IBA + 1.0 g.L <-1 > AC + 7.8 g.L <-1 > Ag + 30.0 g.L <-1 > Su + 100.0 g.L <-1 > coconut meat.

The present invention discloses a coding sequence for the AP1 flowering regulatory gene in Chinese orchids and its application. This orchid floral organ development regulatory gene was isolated from the floral organ cDNA of the Chinese orchid cultivar 'Bai Mo'. Increasing its expression can cause loss of apical dominance in Arabidopsis thaliana, an increase in lateral inflorescences, irregular arrangement of florets on the inflorescence axis, and abnormal petal development in flowers, with the occurrence of fewer petals or petals transforming into sepals. In Chinese orchids, a significant positive correlation was found between gene expression and an increase in sepals, and expression was significantly elevated in variants with a tree-forked flower pattern, indicating that this gene promotes sepal development in Chinese orchids. The Chinese orchid flowering regulatory gene disclosed herein can be used to study the molecular mechanisms of floral organ development in orchidaceae plants and improve flowering traits in plants.

The invention discloses a Chinese orchid growth regulation gene CsARF4 as well as a coding sequence and application thereof, and belongs to the field of plant molecular biology. Analysis finds that the expression of CsARF4 in Chinese cymbidium roots is the highest; in different flower development stages of cymbidium sinense, the expression trend of CsARF4 is gradually reduced; in tissue parts of mature flowers of cymbidium sinense, the expression of CsARF4 in petals is the highest. Through a genetic engineering technology, it is found that overexpression of the Chinese cymbidium growth and development regulation gene in cymbidium sinense can change plant growth traits and cause increase and rapid growth of rhizomes, so that the gene and a protein coded by the gene can be used for rhizome proliferation, the budding rate is increased, and the yield of plants is increased. Research on growth and development regulation ways such as rapid growth of plants is promoted, and the method is used for improving growth traits.

The invention relates to the field of molecular breeding, in particular to a KASP marker combination for identifying a cymbidium sinense floribunda odd flower variety as well as application, primers and a kit of the KASP marker combination. The KASP marker comprises one or more of KASP1, KASP2 and KASP2, and the KASP marker comprises one or more of KASP1, KASP2 and KASP2. The three KASP markers designed and developed by the invention can be used for breeding of cymbidium sinense floribunda odd-flower germplasm and filial generation, and can accurately perform genetic typing on cymbidium sinense varieties with floribunda number greater than 6 and normal cymbidium sinense varieties, and the molecular marker combination can play a role in large-scale early germplasm screening. And effective assistance is provided for molecular breeding of the cymbidium sinense type.

The invention relates to the technical field of gene engineering, in particular to a Chinese orchid CsPDS gene and application thereof, a protein, a silence vector and a method. The nucleotide sequence of the Chinese orchid CsPDS gene is as shown in SEQ ID No. 1. The silent Chinese orchid CsPDS gene can obtain a Chinese orchid phenotype albino plant, the silent vector can effectively reduce the expression level of the Chinese orchid CsPDS gene, the Chinese orchid CsPDS gene can be systematically diffused and spread in the Chinese orchid plant, and an important technical support is provided for functional verification of the Chinese orchid gene.