47 results about "Cymbidium sinense" patented technology

The invention provides a compound fungicide. The compound fungicide comprises main agents and auxiliary agents, wherein the main agents include difenoconazole and paenibacillus polymyxa, difenoconazole accounts for 1-80% of the total compound fungicide by weight, and the addition of paenibacillus polymyxa is 1*10<6>-1*10<8>cfu per milligram of compound fungicide. The compound fungicide has the advantages that the fast-acting property of the microbial fungicide can be improved and the pesticide residues can be reduced by adopting the biocontrol bacterium and chemical fungicide mixture, so that the compound fungicide meets the requirement of sustainable development and the requirements of current agricultural production, is environment-friendly and residue-free, is beneficial to delaying generation of drug resistance of germs, and especially has good control effects on blight, caused by fusarium oxysporum, of orchids such as cymbidium sinense, cymbidium ensifolium and the like.

The invention discloses a method for creating easily-industrialized breeding Cymbidium sinense resources, belonging to the field of innovation of plant germplasm resources. The method comprises the following steps: selecting hybrid Cymbidium containing a Cymbidium sinense genome as core parents so as to prepare a hybrid combination, carrying out single-seed in-vitro culturing and evaluating the type and the tissue culturing and rapid propagation abilities of an intermediate propagule of hybrid generations, producing test-tube seedlings, identifying produced test-tube seedlings, and carrying out transplanting so as to obtain the easily-industrialized breeding Cymbidium sinense resources. Compared with a conventional breeding method which selects Cymbidium sinense resources after transplanting of grown-up seedlings, the method provided by the invention completes selection of the easily-industrialized Cymbidium sinense resources in test tubes, so the period of breeding is significantly shortened, and manpower and material resources are saved; meanwhile, the method can really improve the tissue culturing and rapid propagation abilities of traditional Cymbidium sinense, obtains the easily-industrialized breeding Cymbidium sinense resources, realizes industrialized production of Cymbidium sinense seedlings, and has important practical significance on promoting the industrial development of Cymbidium sinense.

The invention relates to the technical field of prevention of pests and diseases of traditional Chinese medicines, and particularly relates to a preparation for preventing anthracnose of rhizoma paris, and a preparation method thereof. The preparation contains the following raw materials: cymbidium sinense, rabdosia rubesens, pumpkin stems, thesium chinense, humifuse euphorbia herb, tea tree essential oil, chili stems and complex microorganisms. The preparation method comprises the following steps: firstly, mixing cymbidium sinense, humifuse euphorbia herb, chili stems with sweet water, then inoculating lactobacillus bulgaricus, performing constant-temperature soaking, then inoculating bacillus amyloliquefaciens and bacillus subtitles, performing secondary constant-temperature soaking, and filtering to obtain a soaking solution for later use; mixing thesium chinense, rabdosia rubesens and pumpkin stems, adding water for extraction, performing ultrasonic processing, and filtering to obtain extracting liquid for later use; evenly mixing the soaking solution, the extracting liquid and the tea tree essential oil, and evenly mixing with new high-fat film powder after performing oscillation and ultrasonic processing, so as to obtain the preparation. The preparation has balanced nutrients, is safe and non-toxic, and has better inhibition effect on harmful bacteria, especially has obvious inhibition effect on the bacillus anthracis.

The invention discloses a rapid propagation method for hybrid cymbidium seedlings of cymbidium sinense and cymbidium hookerianum. Hybrid cymbidium aseptic seedling base tissue of the cymbidium sinense and the cymbidium hookerianum is used as a raw material, induction culture is performed in an induction culture medium containing various plant growth regulators and additives, protocorms are obtained and subjected to proliferation and differentiation culture, and then seedling strengthening, rooting and transplantation are performed. Steps of proliferation and differentiation of the protocorms are combined, the culture procedure is simple, the material cost for preparation of the culture medium is greatly reduced, the labor cost for preparation of the culture medium and inoculation is also reduced, the inductivity of the protocorms is high, the quantity of the protocorms is large, the proliferation coefficient is large, and the method has multiple advantages of low production cost, high seedling quality, short culture period, avoidance of variation and the like.

The invention relates to the technical field of plant cultivation and reproduction, in particular to a method for cultivation and reproduction of orchids, comprising the following steps: material selection, material disinfection, induction cultivation, proliferation cultivation, rooting cultivation, and transplanting; the cultivated seedlings can be made The plant has a high seedling rate, strong adaptability after cultivation, and the seedlings grow well. Through biotechnology means, it meets the needs of commercial production in a short period of time, and provides technical support for the scale and industrialization of enterprises. The cost of the present invention is low, with 1/2MS medium as basic mother solution, and adopts other nutrients to replace expensive coconut juice as nutrient solution, the consumption of its macroelement is half of the macronutrient consumption of standard MS medium, in Under the premise that the average leaf length, the average elongated height of buds and the percentage of large buds are not affected, the preparation cost of the medium is significantly reduced.

The invention discloses a special culture medium for cymbidium sinense. The special culture medium comprises, by weight, 70-80 parts of Heller culture media, 15-20 parts of ferric sulfate, 8-10 parts of disodium EDTA (ethylene diamine tetraacetic acid), 6-8 parts of glucose, 8-10 parts of lactalbumin hydrolysate, 8-12 parts of maize endosperm, 6-12 parts of tomato juice, 3-12 parts of coconut juice, 3-5 parts of indoleacetic acid, 3-6 parts of indolebutyric acid, 2-5 parts of naphthalene acetic acid, 3-6 parts of naphthoxyacetic acid, 1-5 parts of ABT rooting powder, 1-4 parts of zeatin, 1-4 parts of kinetin, 1-3 parts of isopentene aminopurine, 1-3 parts of gibberellin, 20-30 parts of agar, 5-15 parts of activated carbon and 40-50 parts of water. The special culture medium for the cymbidium sinense has the advantages that the special culture medium contains rich nutrients, is manufactured for characteristics of cymbidium sinense plants and has an excellent market prospect, and the survival rate of the cymbidium sinense plants can be effectively increased.

The invention discloses bacterium paenibacillus polymyma SHL-1 and an application thereof in preventing and controlling stalk rot of cymbidium sinense. The bacterium paenibacillus polymyma SHL-1 with a preservation number of CCTCCM2011441 is preserved in the China Center for Type Culture Collection on December 2, 2011. In the invention, the bacterium paenibacillus polymyma SHL-1 is inoculated to an LB (Langmuir-Blodgett) liquid culture medium with pH of 7.0, and is cultured for 48 hours in a oscillation way at 200r/min at a temperature of 28 DEG C, so that a biological preparation can be prepared. The biological preparation has a strong inhibition effect to the stalk rot of cymbidium sinense, and has environmentally-friendly non-toxic sources and small influence to biological environment;and furthermore, the biological preparation has low requirements to the culture condition, has an inhibition effect to various pathogenic fungi, and has good development and application prospects.

The invention discloses a method for regulating the flowering period of cymbidium sinense. The method comprises the steps of light temperature management and water and fertilizer management before flower forcing and application of a plant growth regulator for mixed treatment to promote flower bud differentiation and development. By means of the method, the initial flowering stage of the floweringperiod of the cymbidium sinense can be advanced, the flowering rate in the flowering period is increased, the consistency of the flowering period is improved, and the problems of flowering irregularity and flowering period inconsistency of the cymbidium sinense are solved; the method is easy to operate, low in cost and convenient to implement; the method achieves the purpose of regulating the flowering period of the cymbidium sinense, so that the flowering period, dispersed before and after the Lantern Festival, of the cymbidium sinense is stabilized to come before the Spring Festival, the application of the cymbidium sinense serving as festival flowers is achieved, the demands of the market are met accordingly, and the economic benefits are outstanding.

The invention discloses a bacillus subtilis subsp. Deserticola strain and application thereof. The bacillus subtilis subsp. Deserticola strain is classified and named as Bacillus subtilis subsp. inaquorum, the preservation unit is China General Microbiological Culture Collection Center (CGMCC), the preservation date is January 13th, 2020, the preservation number is 19347, and the bacillus subtilis subsp. Deserticola strain belongs to the bacteria field, phylum firmicutes, bacillus classes, bacillus families, bacillus families and bacillus sp. The application is the application of the bacillus subtilis subsp. Deserticola strain in biological prevention and control of cymbidium sinense root rot.

The invention discloses a CsCoL1 gene relative to cymbidium sinense photoperiod and the application of the CsCoL1 gene. The nucleotide sequence of the CsCoL1 gene is shown from 40th site base to 1020th site base in SEQ ID (sequence identity) NO.1; the nucleotide sequence of encoding protein of the CsCoL1 gene is shown in SEQ ID NO.2; a recombinant plant expression vector contains the CsCoL1 gene;in addition, the CsCoL1 gene is applied to shortening the juvenile period of plants and promoting the plants to bloom. The CsCoL1 gene is cloned from a CONSTANS-like gene-CsCoL1 gene in cymbidium sinense, can be proved to influence the growth and development program of the plants through experiments, and can promote the plants to bloom. Therefore, the CsCoL1 gene of cymbidium sinense provides an effective technical means to improve the growth program of the plants in gene engineering as well as adjust and control the blooming period of the plants, and achieves a broad application prospect anda great economic value.

The invention discloses a method for promoting bud differentiation in the Qijian white cymbidium sinense tissue culture process. The rhizome of Qijian white cymbidium sinense is inoculated into a buddifferentiation culture medium to be cultured, the bud differentiation culture medium contains a biosynthetic flaudio-videoonoid monooxygenase specific inhibitor Yucasin containing cytokinin 6-BA andcatalytic auxin, the expression of hormone related genes is promoted, the changes of an endogenous hormone micro-environment are caused, and therefore the bud differentiation of the rhizome of the Qijian white cymbidium sinense is promoted, and the method can be directly used for factory production of Qijian white cymbidium sinense seedlings and promoting the industrial development of cymbidium sinense.

The invention relates to the technical field of planting materials of orchids, and specifically relates to a planting material for improving the growth of orchids. The formula of the planting materialincludes the following components: in parts by mass, 30-45 parts of bark, 30-40 parts of peanut shells, 2-5 parts of orchid cultivating stone, 3-8 parts of plant ash, 8-15 parts of vesuvianite, 3-8 parts of vermiculite, 3-8 parts of Quercus spinosa David ex Fr. Leaves and 5-10 parts of potassium humate. The invention also discloses a preparation method of the planting material for promotion of the growth of orchids. Through optimization of the formula and the preparation method, good promotion effects on stages of the orchid growth are achieved through the prepared planting material, good effects of water permeability and air permeability are achieved, and the fertilizer effect can last for a long period of time; the prepared planting material has good application effects on different types of orchids, and the application effects on Cymbidium goeringii, Cymbidium faberi, Cymbidium ensifolium, Cymbidium sinense and Cymbidium kanran are more significant; and meanwhile, the adopted raw materials of the planting material is simple, and fast seedling emergence, small dosage of the planting material and cost saving are achieved during growth of the orchids.

The invention discloses a cymbidium sinense seedling-raising method which comprises the following steps: 1) performing piece-root grafting, namely selecting winter or early spring; 2) scion seed tree pretreatment, namely performing shading treatment on the seed tree; 3) selecting and treating stock, namely selecting the main root and thick lateral roots of the seedling, soaking with an activated carbon solution, cleaning the surface and airing to serve as the stock; 4) performing scion selection and treatment, namely selecting healthy annual branches as scions; 5) performing bandage treatment, namely pruning the scions to form a wedged surface, cutting the scions on the cross section of the stock, inserting the scions, and winding and binding up; 5) raising the seedlings, namely storing the scions in a seed bed; and 7) performing field planting, namely enabling terminal buds of the scions to be exposed out of the ground while field planting, watering and fertilizing. According to the cymbidium sinense seedling-raising method disclosed by the invention, due to the pretreatment of the stock and the scions, the problems that the cymbidium sinense cut is oxidized and browned and causes necrosis, cutting or grafting rooting is difficult, the survival rate is low and the like are effectively solved, and the time, land and manual labor special for cultivating the stock can be saved.

The invention provides a supersonic wave wall breaking culture method for Chinese terrestrial orchid progenitor cell embryo. The method comprises the following steps: disinfecting non-cracking orchid fruit with 75% alcohol and taking out seeds; immersing for 5-10 min with 10% sodium hypochlorite; taking out and flushing with sterile water three times for surface disinfection; cutting the fruit on an aseptic bench; pouring progenitor cell embryo powder particle into sterile water; rotating on a rotation shaking table to promote affinity with water; pouring into aseptic supersonic wave oscillation container and oscillating repeatedly until plume seed coat is observed to be ruptured by supersonic wave energy by a stereo microscope; sucking into a prepared medium for aseptic culture. An oscillation mode is as below: oscillating for 1 min and standing for 3 min; then oscillating for 1 min and standing for 3 min; and repeating the process. The invention can start progenitor cell embryo differentiation germination of Chinese terrestrial orchids, such as cymbidium, cymbidium kanran makino, green orchid, qiuzhi, cymbidium sinense and torchlily to culture complete plant, and has great significance for realizing artificial breeding cultivation of rare orchids and saving endangered species.

The invention provides a cymbidium sinense 'labellum and petal sepalody' character-related functional molecular marker. The functional molecular marker is M4wt or M4m, the nucleotide sequence of the M4wt is shown in SEQ ID NO: 1, and the nucleotide sequence of the M4m is shown in SEQ ID NO: 2. By separating and comparing related gene sequences of multiple varieties of cymbidium sinense, a functional specificity site, which is different from other varieties of cymbidium sinense, in 'labellum and petal sepalody' varieties 'Lvyun' and 'Taiyanghua' is obtained. Based on difference of the site, three specific primers M4F1, M4F2 and M4R are designed. A primer pair of M4F1 and M4R and a primer pair of M4F2 and M4R are utilized to amplify the total DNA of the genome of the cymbidium sinense, then agarose gel electrophoresis detection is performed, and thus the 'labellum and petal sepalody' varieties can be quickly identified. The method provided by the invention has great value and meaning in identification and protection of germplasm resources of cymbidium sinense, molecular marker-assisted breeding and molecular design breeding.

The invention provides an insecticidal composition for preventing and treating cymbidium sinense, the effective components of the insecticidal composition are metarhizium anisopliae and tetrachlorantraniliprole, and the ratio of the number of conidia of metarhizium anisopliae to the gram number of tetrachlorantraniliprole is (26-1) * 2 billion: (1-5). The metarhizium anisopliae and the tetrachlorantraniliprole are compounded to achieve a remarkable synergistic effect, the control effect is very good, the use amount of chemical pesticides can be reduced, and environmental pollution is reduced.

The invention discloses cymbidium sinense chlorophyll catabolism regulatory protein as well as a coding gene and application thereof. The amino acid sequence of the cymbidium sinense chlorophyll catabolism regulatory protein disclosed by the invention comprises 307 amino acid residues and is shown in SEQ ID NO:2; the nucleotide sequence of a cymbidium sinense chlorophyll catabolism regulatory gene for coding the cymbidium sinense chlorophyll catabolism regulatory protein comprises 924 basic groups and is shown in SEQ ID NO:1. The cymbidium sinense chlorophyll catabolism regulatory protein disclosed by the invention has the activity of chlorophyll degrading enzyme; after the expression quantity of the cymbidium sinense chlorophyll catabolism regulatory protein is increased, the content of chlorophyll in arabidopsis thaliana leaf blades can be decreased; after the cymbidium sinense chlorophyll catabolism regulatory protein is highly expressed in cymbidium sinense, the contents of chlorophyll in leaf blades and flowering branches of cymbidium sinense are obviously decreased. The cymbidium sinense chlorophyll catabolism regulatory gene and the cymbidium sinense chlorophyll catabolism regulatory protein coded by the same can be used for research on a chlorophyll degradation molecular mechanism and character improvement of leaf color or flower color of plants.

The invention relates to a method for manufacturing fresh cutting flower of orchid, the steps of which are that: firstly, scissors, a container, a sponge, a flower mud and gravels are sterilized by potassium permanganate solution with concentration of two thousandth; secondly, river sand, the gravels and nutrient solution are put into the container and scape of the orchid is cut down by the scissors with a bevel of 15 to 65 degrees and is inserted into the container within 1 hour; thirdly, fresh orchid leaves are cleaned and inserted into the container; then the mouth of the container is sealed by the sponge or the flower mud. The nutrient solution is prepared by 1 to 30 weight percentage of glucose, 0 to 5 weight percentage of potassium dihydrogen phosphate and 68 to 98 weight percentage of water. The method for manufacturing the fresh cutting flower of the orchid of the invention solves the problem that the scape of the orchid can not flower for a plurality of days normally after leaving the parent matrix. The practice proves that the orchid can flower naturally for 15 to 30 days and emit fragrance continuously by using the method of the invention and by choosing Cymbidium sinense flowering from January to March, Cymbidium flowering from March to April, Cymbidium ensifolium flowering from June to August and Smoothlip cymbidium flowering from October to November.

The invention discloses a culture method for prolonging the flowering period of cymbidium sinense. According to the method, an AMF and DSE inoculated culture medium is added to planting soil in the process of flower bud differentiation, a selenium-containing yeast culture medium is added to the planting soil at an early stage of flower development, and the temperature and the illumination time arecontrolled at early and middle stages of flower development. The culture method can significantly prolong the flowering period of cymbidium sinense, the flowering period is stable, the flowering amount is large and the flower color is gorgeous.

The invention discloses a Chinese orchid flower organ development regulation gene, its encoded protein and its application. The Chinese orchid flower organ development regulatory gene is isolated from the flower organ cDNA of the Chinese orchid Molan variety "Dharma" (Cymbidium sinense), and its nucleotide sequence is shown in SEQ ID NO: 1; the described The amino acid sequence of the Chinese orchid organ development regulatory protein is shown in SEQ ID NO:2. The present invention finds through genetic engineering technology that the overexpression of the Chinese orchid organ development regulation gene in Arabidopsis thaliana and orchids can change the flower shape of the plant, causing the petals to curl, and the orchid petals to transform into lips, and the properties of the lips are strengthened. Therefore, The gene and the encoded protein can be used in the research on the regulation pathway of the flower organ development of orchid plants, and in the improvement of the flower petal shape of the flowering traits of the plants.

A cymbidium sinense tissue culture method includes steps of side bud obtaining, disinfection, induced culture, multiplication culture, differentiation culture, rooting culture and seedling hardening and transplanting. The method has the advantages that cymbidium sinense tissue culture induced side bud explants are obtained in a greenhouse which is relatively stable and clean, and a method of drying before material getting and disinfection pretreatment by enabling materials to enter a sterile environment directly after material getting is adopted, so that lowering of contamination rate is facilitated; the side bud explants selected for cymbidium sinense tissue culture inducing are newly growing second-generation buds, thereby being slight in bacteria breeding, high in activity and conduciveto inducing of rhizome, so that inducing success rate is increased; a rhizome material of Phi0.32-0.38cm in thickness and 3.5-5.5cm in length obtained after cymbidium sinense tissue culture rhizome differentiation, selection and multiplication is high in differentiation ability, thereby being conductive to increasing differentiation rate and obtaining robust differentiated seedlings.

The invention discloses an anti-hair loss care composition, and relates to the technical field of anti-hair loss hair care. The hair care composition is prepared from the following components in partsby weight: 1-5 parts of loquat leaf extract, 1-10 parts of biotin tripeptide-1, 1-10 parts of acetyl tetrapeptide-3, 1-5 parts of hydrolyzed pea protein and 1-5 parts of cymbidium sinense leaf extract. The composition provided by the invention can radically solve the problem of hair loss from the source of hair loss, has no side effect on the body, and is derived from green natural components.