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Method for identifying molecules of recessive white feather genes of chicken

A recessive white feather and molecular identification technology, applied in the field of animal genetics and breeding, can solve the problems of long time and achieve the effect of expanding the population size and shortening the breeding time

Inactive Publication Date: 2010-04-21
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the long time defects of traditional breeding methods, to find a fast and reliable molecular biological method for identification of chicken recessive white feather gene

Method used

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  • Method for identifying molecules of recessive white feather genes of chicken
  • Method for identifying molecules of recessive white feather genes of chicken
  • Method for identifying molecules of recessive white feather genes of chicken

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Sample preparation and storage

[0020] Get 100ul blood of white-feathered silkies in Yunyang County, Hubei Province, China, containing anticoagulants, and use the commonly used phenol-chloroform crude extraction method (Sambrook J, Fritsch EF, Maniartis T. Molecular cloning Experiment Guide [M]. 2nd Edition. Jin Dongyan, Li Mengfeng. Beijing: Science Press, 1999.465-467) Extract blood DNA from white feather silky chicken, and store the DNA sample in a 4°C refrigerator for later use.

[0021] 2. Primer Design

[0022] The primer design of this example is shown in Table 1.

[0023] Table 1 PCR amplification primers designed by the present invention

[0024] Primer

effect

forward primer

reverse primer

size

primer pair 1

Check for retroviruses

Virus insertion

5'GTTGCCTCT

GGCTCTATT 3'

5' TTTCTGTGAGT

AAGGGTTG 3'

292bp

primer pair 2

Detect if a TYR base

due to control

5'TTGTTGGAG ...

Embodiment 2

[0033] 1. Sample preparation and storage

[0034] Take 100ul blood of white legion chicken containing anticoagulant, and use common phenol-chloroform crude extraction method (Sambrook J, Fritsch EF, Manny Artis T. Molecular Cloning Experiment Guide [M]. No. 2 Edition. Jin Dongyan, Li Mengfeng. Beijing: Science Press, 1999.465-467) extract blood DNA, and store the DNA sample in a 4°C refrigerator for later use.

[0035] 2. Primer Design

[0036] The primer design of this example is shown in Table 2.

[0037] Table 2 PCR amplification primers designed by the present invention

[0038] Primer

effect

Forward

reverse

size

primer pair 1

Check for retroviruses

Virus insertion

5'GTTGCCTCT

GGCTCTATT 3'

5' TTTCTGTGAGT

AAGGGTTG 3'

292bp

[0039] Primer

effect

Forward

reverse

size

primer pair 2

Detect if a TYR base

due to control

5'TTGTTGGAG

GCTGGGTAT 3'

...

Embodiment 3

[0048] 1. Sample preparation and storage

[0049] The blood was collected from Xinhua Chicken of Hubei Xinhua Ecological Livestock and Poultry Development Co., Ltd., and 100ul blood containing anticoagulant was used for crude extraction with phenol-chloroform (Sambrook J, Fritsch EF, Maniati Si T. Molecular Cloning Experiment Guide [M]. 2nd Edition. Jin Dongyan, Li Mengfeng. Beijing: Science Press, 1999.465-467) Extract blood DNA, and store the DNA sample in a 4°C refrigerator for later use.

[0050] 2. Primer Design

[0051] The primers used in this example are listed in Table 3.

[0052] Table 3 PCR amplification primers designed by the present invention

[0053] Primer

effect

forward primer

reverse primer

size

primer pair 1

Check for retroviruses

Virus insertion

5'GTTGCCTCT

GGCTCTATT 3'

5' TTTCTGTGAGT

AAGGGTTG 3'

292bp

[0054] Primer

effect

forward primer

reverse primer

...

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PUM

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Abstract

The invention belongs to the technical field of chicken molecule mark preparation, and in particular relates to and builds a method for identifying molecules of recessive white feather genes of chicken. In the method for identifying the molecules of the recessive white feather genes of the chicken, nucleotide sequences of recessive white feather control genes of the chicken (TYR genes inserted with retroviruses) and colored feather control genes TYR genes are utilized to design a primer pair 1 and a primer pair 2 respectively, wherein the primer pair 1 is used for detecting recessive white feather allelic genes; and the primer pair 2 is used for detecting colored feather allelic genes. The method comprises the following steps: extracting genomic DNA of the chicken to be identified and performing PCR amplification by using the primer pairs 1 and 2; performing electrophoresis detection on amplification products; judging the type of the feather color genes of the chicken according to electrophoresis results; if the amplification products are produced by only the primer pair 1, determining the feather color genes are recessive white feather homozygote (the phenotype is a white feather); if the amplification products are produced by only the primer pair 2, determining the feather color genes are non-recessive white feather homozygote (the phenotype is either a colored feather or the white feather); and if the amplification products are produced by both of the primer pairs 1 and 2, determining the feather color genes are recessive white feather heterozygote (the phenotype is either a colored feather or the white feather). The invention provides the quick and convenient method for identifying the molecules of the recessive white feather genes of the chicken.

Description

technical field [0001] The invention relates to the field of animal genetics and breeding, in particular to a molecular identification method for a chicken recessive white feather gene. Background technique [0002] With the improvement of living standards, people's consumption concept has changed from the quantity type to the quality type. White-feathered chickens are one of the important symbols of imported broilers and some laying hens (such as white-shell hens). Broilers imported from abroad grow fast and have high feed efficiency, but their meat quality is worse than that of local chickens in China. The high-quality chickens (including broiler chickens and laying hens) produced by Chinese local chickens are more and more favored by consumers. Market demand has promoted the development of high-quality chicken breeding. A good breeding material is very important for breeding. Most chicken breeds imported from abroad are of dominant white feather type, and the offspring o...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 龚炎长彭秀丽李世军俸艳萍杨桓陈忠
Owner HUAZHONG AGRI UNIV
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