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Method for promoting enzymolysis saccharification of lignocellulose

A lignocellulase and lignocellulose technology is applied in the field of promoting lignocellulosic enzymatic hydrolysis and saccharification, which can solve the problems of poor enzyme activity, difficult operation, low saccharification rate, etc., so as to ensure activity, avoid loss, and reduce thermal inactivation. Effect

Active Publication Date: 2017-06-23
YULIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method of using gamma irradiation pretreatment combined with hair protein as an enzymolysis aid to promote enzymolysis and saccharification of lignocellulose, which overcomes the defects of low saccharification rate, poor enzyme activity, high cost, and difficult operation in the prior art.

Method used

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  • Method for promoting enzymolysis saccharification of lignocellulose
  • Method for promoting enzymolysis saccharification of lignocellulose
  • Method for promoting enzymolysis saccharification of lignocellulose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 1) Select wool from the hair, wash the wool with deionized water to remove surface impurities, dry it, and then soak the wool with a solid-to-liquid ratio of 1:1 (mg / mL) in 30% (v / v) acetic acid solution. Treat at 85°C for 30 minutes. Use ammonia water to neutralize excess acetic acid until the pH value is 6, filter, and dry the filtrate at 40°C for 2 days to obtain protein powder. Seal the obtained hair protein powder in a polyethylene bag and store at 4°C for future use.

[0043] 2) Select corn stalks for lignocellulosic biomass. First, take a certain amount of corn stalks, pick out foreign matter, crush and sieve the selected stalks (40 mesh), soak the sieved straw powder in deionized water, solidify The liquid ratio is 0.5:1 (mg / mL), and then irradiated with gamma rays at a dose of 1kGy for 10min.

[0044]3) Take 0.5g of the pretreated sample in (2) and place it in a 50mL flask, add 2mL of 0.075mol / L (pH4.8) citric acid-sodium citrate buffer solution into it and mi...

Embodiment 2

[0051] 1) Select wool from the hair, wash the wool with deionized water to remove surface impurities, dry it, and then soak the wool with a solid-to-liquid ratio of 1:2 (mg / mL) in 35% (v / v) acetic acid solution. Treat at 88°C for 35 minutes. Use ammonia water to neutralize excess acetic acid until the pH value is 6.3, filter, and dry the filtrate at 45°C for 2 days to obtain protein powder. Seal the obtained hair protein powder in a polyethylene bag and store at 4°C for future use.

[0052] 2) Select corn stalks for lignocellulosic biomass. Firstly, take a certain amount of corn stalks, pick out foreign objects, crush and sieve the selected stalks (50 mesh), soak the sieved straw powder in deionized water, solidify The liquid ratio is 1:1 (mg / mL), and then irradiated with gamma rays at a dose of 3kGy for 20min.

[0053] 3) Take 0.5g of the pretreated sample in (2) and place it in a 50mL flask, add 2.5mL of 0.075mol / L (pH4.8) citric acid-sodium citrate buffer to it and mix wel...

Embodiment 3

[0060] 1) Select wool for hair, wash the wool with deionized water to remove surface impurities, dry it, and then soak the wool with a solid-to-liquid ratio of 1:3 (mg / mL) in 40% (v / v) acetic acid solution. Treat at 90°C for 40 minutes. Use ammonia water to neutralize excess acetic acid until the pH value is 6.6, filter, and dry the filtrate at 50°C for 2 days to obtain protein powder. Seal the obtained hair protein powder in a polyethylene bag and store at 4°C for use.

[0061] 2) Select corn stalks for lignocellulosic biomass. First, take a certain amount of corn stalks, pick out foreign matter, crush and sieve the selected stalks (60 mesh), soak the sieved straw powder in deionized water, solidify The liquid ratio is 1.5:1 (mg / mL), and then irradiated with gamma rays with an irradiation dose of 6kGy for 30min.

[0062] 3) Take 0.5g of the pretreated sample in (2) and place it in a 50mL flask, add 3mL of 0.075mol / L (pH4.8) citric acid-sodium citrate buffer solution into it ...

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Abstract

The invention relates to a method for promoting enzymolysis saccharification of lignocellulose. The method comprises the steps of soaking hair in an acetic acid solution which is 85-95 DEG C firstly, regulating the pH value to be 6-7, conducting filtration, and drying filtrate to obtain hair protein powder; then smashing and screening lignocellulose biomass to obtain lignocellulose biomass powder; soaking the lignocellulose biomass powder in deionized water for irradiation treatment; extracting the lignocellulose biomass powder after irradiation treatment, adding a citric acid-sodium citrate buffer solution, conducting uniform mixing to obtain a mixed solution, and adding cellulose and the hair protein powder to the mixed solution to obtain enzymatic hydrolysate; and conducting enzymatic hydrolysis reaction on the enzymatic hydrolysate for 3-24 h at 30-45 DEG C to promote enzymolysis saccharification of lignocellulose. The structure of lignocellulose is changed through irradiation treatment to facilitate enzymolysis saccharification, the hair protein powder is used as an enzymolysis assistant, non-specificity absorption of cellulose by lignin is reduced, and higher efficiency can be realized within a shorter period of time.

Description

[0001] 【Technical field】 [0002] The invention belongs to the field of enzymatic hydrolysis of lignocellulose, and in particular relates to a method for promoting enzymatic hydrolysis and saccharification of lignocellulose. [0003] 【Background technique】 [0004] my country is a large agricultural country with rich lignocellulose resources. Efficient development of this kind of renewable resources is an effective means to alleviate the energy crisis, and it is also of great significance to reduce environmental pollution. Lignocellulose is mainly composed of cellulose, hemicellulose, and lignin. It is stable in nature and difficult to degrade. It is difficult to be directly utilized without effective pretreatment methods to destroy its structure. On the other hand, the hydrolysis and saccharification of biomass after pretreatment is a key link in the utilization of lignocellulosic resources. Among many hydrolysis methods, enzymatic hydrolysis has unique advantages such as thor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14
CPCC12P19/14C12P2201/00
Inventor 相玉琳卢翠英王立鹏相玉秀王若恺
Owner YULIN UNIV
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